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Our goal was to use them as biomarkers of oxidative stress and inflammation and to evaluate their correlation with glaucoma and impact on surgical outcome.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Conjunctival samples were obtained from POAG (<jats:italic>n<\/jats:italic>\u00a0=\u00a014) and ExG (<jats:italic>n<\/jats:italic>\u00a0=\u00a017) patients and from control subjects (<jats:italic>n<\/jats:italic>\u00a0=\u00a011) operated for macular hole, retinal detachment or strabismus. Immunohistochemistry was performed using the antibody against ox\u2010LDL. Lipids and calcium were analysed by histochemical stainings with Nile red and Alizarin red S, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Immunoreaction for ox\u2010LDL was significantly increased in POAG (p\u00a0= 0.049) and the number of lipid aggregates was significantly higher in ExG (p\u00a0=\u00a00.009) when compared to control. When POAG and ExG patients were grouped according to the outcome of deep sclerectomy (DS) surgery, the number of lipid (p\u00a0&lt;\u00a00.001) and calcium aggregates (p\u00a0=\u00a00.014) were significantly higher in the conjunctival stroma of patients whose surgery failed within a three\u2010year follow\u2010up period.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The lipid\u2010mediated alterations suggested the presence of oxidative stress and inflammation in the conjunctiva of glaucoma patients. The present data further support the role of oxidative stress and inflammation in the wound healing process leading to excessive scarring and failure in DS surgery.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aos.13380","type":"journal-article","created":{"date-parts":[[2017,1,31]],"date-time":"2017-01-31T10:03:04Z","timestamp":1485856984000},"page":"378-385","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Oxidized low\u2010density lipoprotein, lipid and calcium aggregates reveal oxidative stress and inflammation in the conjunctiva of glaucoma patients"],"prefix":"10.1111","volume":"95","author":[{"given":"Minna","family":"Helin\u2010Toiviainen","sequence":"first","affiliation":[{"name":"Department of Ophthalmology Institute of Clinical Medicine University of Eastern Finland Kuopio 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