{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T10:16:34Z","timestamp":1777889794399,"version":"3.51.4"},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,6,21]],"date-time":"2017-06-21T00:00:00Z","timestamp":1498003200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Ophthalmologica"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>To study the performance of a completely synthetic organ culture (OC) preservation system containing recombinant human serum albumin (<jats:styled-content style=\"fixed-case\">rHSA<\/jats:styled-content>) for preservation of human donor corneas.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Twenty\u2010four paired donor corneas were randomly collected, and one cornea from each donor was preserved in synthetic (experimental) and serum\u2010based media (control). The tissues were assessed at day 0; after 6\u00a0days of preservation at room temperature (<jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>) in Cornea Trans<jats:sup>\u00ae<\/jats:sup> and Cornea Prep <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content><jats:sup>\u00ae<\/jats:sup>; after 28\u00a0days at 31\u00b0C in Cornea Syn<jats:sup>\u00ae<\/jats:sup> [with <jats:styled-content style=\"fixed-case\">rHSA<\/jats:styled-content>] and Cornea Max<jats:sup>\u00ae<\/jats:sup> [with foetal calf serum (FCS)] and; 4\u2010day post deswelling in Cornea Trans<jats:sup>\u00ae<\/jats:sup> and Cornea Jet<jats:sup>\u00ae<\/jats:sup>. Thickness was determined with optical coherence tomography (<jats:styled-content style=\"fixed-case\">OCT<\/jats:styled-content>) and transparency with a validated, custom device. Morphology, endothelial cell density (<jats:styled-content style=\"fixed-case\">ECD<\/jats:styled-content>) and mortality were observed after treating the tissues with Trypan blue and sucrose. Glucose uptake by the cells was analysed. Data were compared using non\u2010parametric paired Wilcoxon tests with p\u00a0<jats:italic>&lt;<\/jats:italic>\u00a00.05 deemed significant. Histology using periodic acid\u2010schiff (<jats:styled-content style=\"fixed-case\">PAS<\/jats:styled-content>), expressions of p63, <jats:styled-content style=\"fixed-case\">CK<\/jats:styled-content>12, <jats:italic>\u03b1<\/jats:italic><jats:styled-content style=\"fixed-case\">SMA<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">ZO<\/jats:styled-content>\u20101 were analysed, and cell apoptosis postpreservation was studied.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Corneas stored in synthetic media showed a higher and statistically significant value as compared to serum\u2010based media in terms of viable endothelial cell density (<jats:styled-content style=\"fixed-case\">VECD<\/jats:styled-content>), mortality, morphology and glucose uptake postpreservation. Histology showed presence of all the layers, all the markers were expressed, and no apoptosis was observed in either media.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The new synthetic preservation system containing <jats:styled-content style=\"fixed-case\">rHSA<\/jats:styled-content> (and other confidential constituents) showed better preservation effects than traditional media containing serum.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aos.13498","type":"journal-article","created":{"date-parts":[[2017,6,21]],"date-time":"2017-06-21T09:59:08Z","timestamp":1498039148000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Recombinant human serum albumin for corneal preservation"],"prefix":"10.1111","volume":"96","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5186-068X","authenticated-orcid":false,"given":"Mohit","family":"Parekh","sequence":"first","affiliation":[{"name":"International 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