{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T22:22:44Z","timestamp":1758925364846,"version":"3.37.3"},"reference-count":28,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,11,29]],"date-time":"2017-11-29T00:00:00Z","timestamp":1511913600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Ophthalmologica"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>To study the morphology of human corneal nerves in eye bank organ\u2010cultured corneas and in corneal grafts post\u2010transplantation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Thirty\u2010seven organ\u2010cultured corneas were divided into: Group\u2010A, anterior 300\u2013400\u00a0<jats:italic>\u03bc<\/jats:italic>m of 20 corneas used for Descemets stripping endothelial keratoplasty, and Group\u2010B, 17 full\u2010thickness corneas unsuitable for transplantation. Corneas whole mounts were stained for nerves using acetylcholinesterase technique and examined by NanoZoomer digital pathology microscope. Central and sub\u2010Bowman's stromal nerves and the sub\u2010basal nerve plexus including perforation sites and terminal bulbs were studied. Ten eyes were imaged following penetrating keratoplasty using <jats:italic>in\u2010vivo<\/jats:italic> confocal microscopy (<jats:styled-content style=\"fixed-case\">IVCM<\/jats:styled-content>) for the presence of sub\u2010basal and stromal nerves at 1, 4\u20135 and 7\u20138\u00a0weeks postoperatively (five eyes) and in all the other five eyes, the final follow\u2010up was at 12\u00a0weeks.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Fifteen of twenty (75%) corneas had stromal nerves in Group\u2010A and 15 of 17 (88.2%) in Group\u2010B. Average number of stromal nerves entering peripherally were 9.1 (range: 1\u201336). 7.5 in Group\u2010A and 10.8 in Group\u2010B. Central stromal nerves were seen in eight samples in Group\u2010A and nine in Group\u2010B. Many stromal nerves terminated abruptly without demonstrable continuity through Bowman's membrane. No terminal bulbs or sub\u2010basal nerves were detected. <jats:italic>In\u2010vivo<\/jats:italic> confocal microscopy (<jats:styled-content style=\"fixed-case\">IVCM<\/jats:styled-content>) showed 4 of 5 in 9 of 10 (90%) donor corneas had stromal nerves 1\u00a0week postoperatively, which remained present in 8 of 10 (80%) corneas at 4\u20135\u00a0weeks and in 9 of 10 (90%) at 7\u20138\u00a0weeks postoperatively. All 5 corneas analysed at 12\u00a0weeks showed the same stromal nerves from 1 to 12\u00a0weeks postoperatively. Sub\u2010basal nerves were absent in all corneas over the 12\u2010week study period.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study provides further insight into the behaviour of corneal nerves in transplanted corneas. Corneal stromal nerves\/nerve\u2013sheaths are preserved in organ\u2010cultured eye bank eyes and persist post\u2010transplantation up to 3\u00a0months. These could provide directional guidance to regenerating nerves from host stroma.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aos.13614","type":"journal-article","created":{"date-parts":[[2017,11,29]],"date-time":"2017-11-29T17:37:16Z","timestamp":1511977036000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Survival of corneal nerve\/sheath structures in organ\u2010cultured donor corneas"],"prefix":"10.1111","volume":"96","author":[{"given":"Virinder K.","family":"Dhillon","sequence":"first","affiliation":[{"name":"Department of Ophthalmology Section\u00a0of Academic Ophthalmology Division of Clinical Neuroscience University of Nottingham Nottingham UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6796-9340","authenticated-orcid":false,"given":"Mohamed S.","family":"Elalfy","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Section\u00a0of 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