{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T05:38:48Z","timestamp":1775021928879,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,1,16]],"date-time":"2018-01-16T00:00:00Z","timestamp":1516060800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Eye Tumour Research Fund, Charitable Funds, Royal Liverpool University Hospital NHS Trust","award":["A0928\/CF"],"award-info":[{"award-number":["A0928\/CF"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Ophthalmologica"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>Nestin, a member of the intermediate filament protein family, has been described as a putative cancer stem cell marker (<jats:styled-content style=\"fixed-case\">CSC<\/jats:styled-content>) in uveal melanoma and poor prognostic factor in a variety of tumours, including cutaneous melanoma. In this study, we examined the expression of nestin in primary (<jats:styled-content style=\"fixed-case\">PUM<\/jats:styled-content>) and metastatic uveal melanoma (<jats:styled-content style=\"fixed-case\">MUM<\/jats:styled-content>) samples, and correlated the findings with histological, clinical and survival data.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Nestin expression was assessed by immunohistochemistry in 141 <jats:styled-content style=\"fixed-case\">PUM<\/jats:styled-content> and 26 <jats:styled-content style=\"fixed-case\">MUM<\/jats:styled-content> samples; 11 <jats:styled-content style=\"fixed-case\">PUM<\/jats:styled-content> cases were matched with their corresponding metastases. The percentage of tumour cells expressing nestin was scored by three independent observers. Statistical analysis of all data was performed with <jats:styled-content style=\"fixed-case\">SPSS<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Nestin expression was identified in both the cytoplasm and membrane of <jats:styled-content style=\"fixed-case\">UM<\/jats:styled-content> cells. Increased expression of nestin in <jats:styled-content style=\"fixed-case\">PUM<\/jats:styled-content> samples was associated with known poor prognostic parameters, including epithelioid cell morphology (p\u00a0&lt;\u00a00.001), closed loops (p\u00a0=\u00a00.001), higher mitotic count (p\u00a0&lt;\u00a00.001), monosomy 3 (p\u00a0=\u00a00.007) and chromosome 8q gain (p\u00a0&lt;\u00a00.001). Primary uveal melanoma (PUM) with nestin expression levels above a cut\u2010off value of 10% [as determined by receiver operating characteristic (ROC) analysis] was associated with a significantly reduced survival time (Log\u2010rank, p\u00a0=\u00a00.002). In <jats:styled-content style=\"fixed-case\">MUM<\/jats:styled-content>, a higher percentage of nestin\u2010positive tumour cells combined with poor prognostic markers in the <jats:styled-content style=\"fixed-case\">PUM<\/jats:styled-content> led to a shorter survival time following the development of metastases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In conclusion, increased nestin expression in <jats:styled-content style=\"fixed-case\">PUM<\/jats:styled-content> is a predictor of a tumour phenotype associated with metastatic progression and reduced survival time at onset of metastasis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aos.13645","type":"journal-article","created":{"date-parts":[[2018,1,16]],"date-time":"2018-01-16T18:11:39Z","timestamp":1516126299000},"page":"503-509","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Nestin expression in primary and metastatic uveal 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UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Goran","family":"Petrovski","sequence":"additional","affiliation":[{"name":"Stem Cells and Eye Research Laboratory Department of Ophthalmology Faculty of Medicine Albert Szent\u2010Gyorgyi Clinical Center University of Szeged Szeged Hungary"},{"name":"Centre for Eye Research Department of Ophthalmology Oslo University Hospital and University of Oslo Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah E.","family":"Coupland","sequence":"additional","affiliation":[{"name":"Department of Molecular and Clinical Cancer Medicine Institute of Translational Medicine University of Liverpool Liverpool 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