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Cells were analysed after two, three and four weeks in culture for <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> strand breaks and oxidized purine bases (Comet assay using the enzyme formamidopyrimidine <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> glycosylase, Fpg) and for expression of <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> repair enzymes <jats:styled-content style=\"fixed-case\">APE<\/jats:styled-content>1, <jats:styled-content style=\"fixed-case\">OGG<\/jats:styled-content>1 and Pol<jats:italic>\u03b2<\/jats:italic> by <jats:italic>in\u00a0situ<\/jats:italic> hybridization (<jats:styled-content style=\"fixed-case\">ISH<\/jats:styled-content>) and by immunohistochemistry (<jats:styled-content style=\"fixed-case\">IHC<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Levels of strand breaks were substantial while levels of net Fpg\u2010sensitive sites (8\u2010oxoguanine and ring\u2010opened FaPy bases) were relatively low in cells engineered in <jats:styled-content style=\"fixed-case\">COM<\/jats:styled-content> and in <jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content>. Both types of medium were found to support expression of base excision repair (<jats:styled-content style=\"fixed-case\">BER<\/jats:styled-content>) enzymes <jats:styled-content style=\"fixed-case\">APE<\/jats:styled-content>1, <jats:styled-content style=\"fixed-case\">OGG<\/jats:styled-content>1 and Pol<jats:italic>\u03b2<\/jats:italic> at the gene level. At the protein level, expression of <jats:styled-content style=\"fixed-case\">APE<\/jats:styled-content>1 and <jats:styled-content style=\"fixed-case\">OGG<\/jats:styled-content>1 was noticeable in both conditions while expression of Pol<jats:italic>\u03b2<\/jats:italic> was low.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our findings indicate low levels of oxidative stress and\/or efficient <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> purine base damage repair in human limbal epithelium engineered in a medium with human serum as the single growth\u2010promoting additive as well as in traditional medium with xenobiotics.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aos.13811","type":"journal-article","created":{"date-parts":[[2018,9,22]],"date-time":"2018-09-22T00:05:57Z","timestamp":1537574757000},"page":"834-840","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Levels of oxidative <scp>DNA<\/scp> damage are low in <i>ex\u00a0vivo<\/i> engineered human limbal epithelial tissue"],"prefix":"10.1111","volume":"96","author":[{"given":"Yolanda","family":"Lorenzo","sequence":"first","affiliation":[{"name":"Center for Eye Research Department of Ophthalmology Oslo University Hospital Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristiane","family":"Haug Berg","sequence":"additional","affiliation":[{"name":"Center for Eye Research Department of Ophthalmology Oslo University Hospital Oslo 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