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Cell survival was assessed by <jats:styled-content style=\"fixed-case\">MTT<\/jats:styled-content> viability assay. All cell lines were seeded to create spheroids and were treated with bleomycin on day 3 and day 8 combined with <jats:styled-content style=\"fixed-case\">EP<\/jats:styled-content>. Spheroids were collected, fixed in buffered formalin and subsequently paraffin embedded for histological assessment of the effects of the treatment on cell viability.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">CM<\/jats:styled-content> cell lines were resistant to electroporation alone and showed a reduction in cell number only when treated with 1000\u00a0Volts\/cm and 8 pulses. <jats:styled-content style=\"fixed-case\">HC<\/jats:styled-content>jE\u2010Gi cells showed higher sensitivity to electric pulses over 750\u00a0Volts\/cm. <jats:styled-content style=\"fixed-case\">MMC<\/jats:styled-content> and 5\u2010<jats:styled-content style=\"fixed-case\">FU<\/jats:styled-content> demonstrated a higher cytotoxicity for the <jats:styled-content style=\"fixed-case\">HC<\/jats:styled-content>jE\u2010Gi cell line. The <jats:styled-content style=\"fixed-case\">CM<\/jats:styled-content> cell lines were resistant to <jats:styled-content style=\"fixed-case\">MMC<\/jats:styled-content> and 5\u2010<jats:styled-content style=\"fixed-case\">FU<\/jats:styled-content>. Bleomycin (1\u00a0<jats:italic>\u03bc<\/jats:italic>g\/ml) alone had no significant effect on the <jats:styled-content style=\"fixed-case\">HC<\/jats:styled-content>jE\u2010Gi even when combined with <jats:styled-content style=\"fixed-case\">EP<\/jats:styled-content> conditions \u2265750\u00a0Volts\/cm. In contrast, it significantly (p \u2010, paired t\u2010test) reduced cell viability in the <jats:styled-content style=\"fixed-case\">CM<\/jats:styled-content> cell lines. Spheroids treated with bleomycin and <jats:styled-content style=\"fixed-case\">EP<\/jats:styled-content> showed a reduction in tumour mass and proliferation rates after treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our <jats:italic>in\u00a0vitro<\/jats:italic> study using 2D and 3D models indicates that the application of <jats:styled-content style=\"fixed-case\">EP<\/jats:styled-content> may effectively enhance chemotherapy with bleomycin in <jats:styled-content style=\"fixed-case\">CM<\/jats:styled-content>. 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