{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T17:19:26Z","timestamp":1788801566935,"version":"build-2803163510"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,9,5]],"date-time":"2019-09-05T00:00:00Z","timestamp":1567641600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Ophthalmologica"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>To assess the <jats:italic>in vitro<\/jats:italic> antimicrobial activity of a new commercial ophthalmic solution containing povidone\u2010iodine 0.6% (<jats:styled-content style=\"fixed-case\">IODIM<\/jats:styled-content><jats:sup>\u00ae<\/jats:sup>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:italic>Staphylococcus aureus <\/jats:italic><jats:styled-content style=\"fixed-case\">ATCC<\/jats:styled-content> 43300, <jats:italic>Pseudomonas aeruginosa <\/jats:italic><jats:styled-content style=\"fixed-case\">ATCC<\/jats:styled-content> 27853, three ocular bacterial isolates (1 <jats:italic>S.\u00a0epidermidis<\/jats:italic>, 1 <jats:italic>S.\u00a0aureus<\/jats:italic>, 1 <jats:italic>P.\u00a0aeruginosa<\/jats:italic>) and five <jats:italic>Candida<\/jats:italic> species were used. The bacterial and fungal isolates were cultured on Columbia blood agar base plates and Sabouraud\u2010dextrose agar plates, respectively and incubated overnight at 37\u00b0C. Bacterial and fungal suspensions in sterile saline solution were prepared to an optical density equal to 0.5 McFarland standard (approximately 10<jats:sup>8<\/jats:sup> <jats:styled-content style=\"fixed-case\">CFU<\/jats:styled-content>\/ml). Suspensions of the isolates were made in <jats:styled-content style=\"fixed-case\">IODIM<\/jats:styled-content><jats:sup>\u00ae<\/jats:sup> solution to obtain a final concentration of 10<jats:sup>6<\/jats:sup> <jats:styled-content style=\"fixed-case\">CFU<\/jats:styled-content>\/ml. The suspensions were then distributed in conical tubes in a final volume of 1\u00a0ml and incubated at 37\u00b0C. At different time\u2010points (1, 5, 10, 15, 20, 25, 30\u00a0min and 24\u00a0hr), 10\u00a0<jats:italic>\u03bc<\/jats:italic>l of each suspension was removed, seeded on Columbia blood agar base and Sabouraud\u2010dextrose agar plates and then incubated for 24\u00a0hr at 37\u00b0C. Positive and negative controls were included in all experiments.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>After 5\u2010min incubation, there was no bacterial growth on any plate. Conversely, <jats:styled-content style=\"fixed-case\">IODIM<\/jats:styled-content><jats:sup>\u00ae<\/jats:sup> failed to kill the <jats:italic>Candida<\/jats:italic> isolates after 30\u00a0min\u2019 exposure and needed 24\u00a0hr to eradicate the organisms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">IODIM<\/jats:styled-content><jats:sup>\u00ae<\/jats:sup> ophthalmic solution showed <jats:italic>in vitro<\/jats:italic> antimicrobial activity against <jats:italic>S.\u00a0epidermidis<\/jats:italic>,<jats:italic> S.\u00a0aureus<\/jats:italic>,<jats:italic> P.\u00a0aeruginosa<\/jats:italic> and <jats:italic>Candida<\/jats:italic> species. Results suggest that it may be a potential candidate for the treatment of ocular surface infections and antimicrobial prophylaxis before intravitreal injections.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aos.14243","type":"journal-article","created":{"date-parts":[[2019,9,5]],"date-time":"2019-09-05T09:12:24Z","timestamp":1567674744000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["<i>In vitro<\/i> antimicrobial activity of a new ophthalmic solution containing povidone\u2010iodine 0.6% (<scp>IODIM<\/scp><sup>\u00ae<\/sup>)"],"prefix":"10.1111","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3052-2662","authenticated-orcid":false,"given":"Antonio","family":"Pinna","sequence":"first","affiliation":[{"name":"Department of Medical, Surgical, and Experimental Sciences University of Sassari Sassari Italy"},{"name":"Azienda Ospedaliero\u2010Universitaria di Sassari Sassari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7681-6194","authenticated-orcid":false,"given":"Matthew Gavino","family":"Donadu","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences University of Sassari Sassari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donatella","family":"Usai","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences University of Sassari Sassari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Dore","sequence":"additional","affiliation":[{"name":"Department of Medical, Surgical, and Experimental Sciences University of Sassari Sassari Italy"},{"name":"Azienda Ospedaliero\u2010Universitaria di Sassari Sassari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9022-4790","authenticated-orcid":false,"given":"Giuseppe","family":"D'Amico\u2010Ricci","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences University of Sassari Sassari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Boscia","sequence":"additional","affiliation":[{"name":"Department of Medical, Surgical, and Experimental Sciences University of Sassari Sassari Italy"},{"name":"Azienda Ospedaliero\u2010Universitaria di Sassari Sassari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefania","family":"Zanetti","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences University of Sassari Sassari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,9,5]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(00)00179-2"},{"key":"e_1_2_5_3_1","unstructured":"American Academy of Ophthalmology(2018):Bacterial keratitis Preferred Practice Patterns. 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