{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T14:45:42Z","timestamp":1783694742147,"version":"3.55.0"},"reference-count":14,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2020,5,14]],"date-time":"2020-05-14T00:00:00Z","timestamp":1589414400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Ophthalmologica"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The current outbreak of COVID\u201019 has spread rapidly all over the world. Respiratory droplets and contaction with infected patients are the two major transmission routes. However, the value of tear virus nucleic acid is still not clear. We dynamic detected the SARS\u2010CoV\u20102 in eye sample of one COVID\u201019 patient with obstruction of common lacrimal ducts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Besides the routine examination, nasopharyngeal and eye swab were continuously measured by polymerase chain reaction assay and next\u2010generation sequencing (NGS). Gene detection was performed for drug use guidance, and flow cytometry was performed to analyse the lymphocyte subsets.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Nasopharyngeal swabs were positive for 22\u00a0days, but eye swabs were still continuously positive for 2\u00a0weeks after nasopharyngeal swabs turned negative. The low level of lymphocyte and the high level IL\u20106 lasted for almost 4\u00a0weeks, then became near normal. Next\u2010generation sequencing (NGS) confirmed the existing of SARS\u2010CoV\u20102, HSV1 and HHV6B virus nucleic acid. The gene detection for drug use guidance showed the genetic locus ABCB1 (3435T&gt;C) rs1045642 belonged to type CC and it mean the efficiency of lopinavir\u2013ritonavir would be significantly decreased. The flow cytometry of lymphocyte subsets showed PD\u20101<jats:sup>+<\/jats:sup>\u00a0CD95<jats:sup>+<\/jats:sup> cells was accounting for 94.8% in CD3<jats:sup>+<\/jats:sup>\u00a0CD8<jats:sup>+<\/jats:sup> T subset and for 94.8% in CD3<jats:sup>+<\/jats:sup>\u00a0TCR\u03b3\u03b4<jats:sup>+<\/jats:sup> T subset.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>As obstruction of common lacrimal duct, positively detection in one eye for 2\u00a0weeks more after nasopharyngeal swab became negative. More eye swabs should be collected from COVID\u201019 patients, especially from those immunocompromised, those with eye symptoms and those had a history of ocular diseases.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aos.14456","type":"journal-article","created":{"date-parts":[[2020,5,14]],"date-time":"2020-05-14T10:19:51Z","timestamp":1589451591000},"page":"859-863","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Positive detection of SARS\u2010CoV\u20102 combined HSV1 and HHV6B virus nucleic acid in tear and conjunctival secretions of a non\u2010conjunctivitis COVID\u201019 patient with obstruction of common lacrimal duct"],"prefix":"10.1111","volume":"98","author":[{"given":"Yaguang","family":"Hu","sequence":"first","affiliation":[{"name":"Department of Ophthalmology The First Affiliated Hospital of Xi\u2019an Jiaotong University Xi\u2019an 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