{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T16:18:38Z","timestamp":1759940318943,"version":"3.40.5"},"reference-count":30,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2022,2,17]],"date-time":"2022-02-17T00:00:00Z","timestamp":1645056000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007397","name":"Univerzita Karlova v Praze","doi-asserted-by":"publisher","award":["GACR 20\u201019278S"],"award-info":[{"award-number":["GACR 20\u201019278S"]}],"id":[{"id":"10.13039\/100007397","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000272","name":"National Institute for Health Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000272","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Ophthalmologica"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>Posterior corneal vesicles (PCVs) have clinical features that are similar to posterior polymorphous corneal dystrophy (PPCD). To help determine whether there is a shared genetic basis, we screened 38 individuals with PCVs for changes in the three genes identified as causative for PPCD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We prospectively recruited patients for this study. We examined all individuals clinically, with their first\u2010degree relatives when available. We used a combination of Sanger and exome sequencing to screen regulatory regions of <jats:italic>OVOL2<\/jats:italic> and <jats:italic>GRHL2,<\/jats:italic> and the entire <jats:italic>ZEB1<\/jats:italic> coding sequence.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The median age at examination was 37.5\u2009years (range 4.7\u201384.0\u2009years), 20 (53%) were male and in 19 (50%) the PCVs were unilateral. Most individuals were discharged to optometric review, but five had follow\u2010up for a median of 12\u2009years (range 5\u201313\u2009years) with no evidence of progression. In cases with unilateral PCVs, there was statistically significant evidence that the change in the affected eye was associated with a lower endothelial cell density (p\u2009=\u20090.0003), greater central corneal thickness (p\u2009=\u20090.0277) and a steeper mean keratometry (p\u2009=\u20090.0034), but not with a higher keratometric astigmatism or a reduced LogMAR visual acuity. First\u2010degree relatives of 13 individuals were available for examination, and in 3 (23%), PCVs were identified. No possibly pathogenic variants were identified in the PPCD\u2010associated genes screened.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We found no evidence that PCVs share the same genetic background as PPCD. In contrast to PPCD, we confirm that PCVs is a mild, non\u2010progressive condition with no requirement for long\u2010term review. However, subsequent cataract surgery can lead to corneal oedema.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aos.15114","type":"journal-article","created":{"date-parts":[[2022,2,17]],"date-time":"2022-02-17T10:32:18Z","timestamp":1645093938000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Posterior corneal vesicles are not associated with the genetic variants that cause posterior polymorphous corneal dystrophy"],"prefix":"10.1111","volume":"100","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7834-8486","authenticated-orcid":false,"given":"Petra","family":"Liskova","sequence":"first","affiliation":[{"name":"Department of Paediatrics and Inherited Metabolic Disorders First Faculty of Medicine Charles University and General University Hospital in Prague  Prague Czech Republic"},{"name":"Department of Ophthalmology, First Faculty of Medicine Charles University and General University Hospital in Prague  Prague Czech 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