{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T22:37:02Z","timestamp":1790462222725,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,8,3]],"date-time":"2022-08-03T00:00:00Z","timestamp":1659484800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004431","name":"Fondation de France","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004431","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004431","name":"Fondation de France","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004431","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100022077","name":"Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement","doi-asserted-by":"publisher","award":["ANR\u201011\u2010LABX\u20100021\u201001"],"award-info":[{"award-number":["ANR\u201011\u2010LABX\u20100021\u201001"]}],"id":[{"id":"10.13039\/501100022077","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Ophthalmologica"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>The aim of this study was to evaluate docosahexaenoic acid (DHA) as a potential antifibrotic agent after glaucoma filtration surgery (GFS) in rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 36 10\u2010week\u2010old Brown Norway rats underwent GFS. Animals were equally divided into three groups: a control group, a DHA group and a mitomycin C (MMC) group. Intraocular pressure (IOP) was measured using a dynamic rebound tonometer, and a photograph of the surgical site was taken on days 1, 3, 7, 10, 14 and 17. The incorporation of DHA into fibroblasts was evaluated by gas chromatography. The expression of alfa\u2010smooth muscle actin (\u03b1\u2010SMA) and Smad proteins was assessed by Western blotting.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>IOP decreased after surgery in animals from the three groups on day 1 after surgery. Over time, IOP remained lower in the DHA and MMC groups than in the control group (median [interquartile range] 8.0 [7.0\u20138.0] and 8.0 [7.3\u20138.0] mmHg vs. 9.0 [8.0\u20139.0] mmHg, respectively; <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). Bleb area in the DHA and MMC groups remained larger than that of the control group from day 7 to day 14 (3.9 [2.9\u20135.2] and 3.5 [2.3\u20134.4] mm<jats:sup>2<\/jats:sup> vs. 2.3 [2.0\u20132.8] mm<jats:sup>2<\/jats:sup>, respectively; <jats:italic>p<\/jats:italic>\u00a0=\u00a00.0021). We did not observe any change in DHA concentrations in the fibroblasts of the DHA group compared with the other groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The impact of DHA on IOP and bleb area was similar to that of MMC. The mechanisms of action of DHA in rat eye fibroblasts deserve further investigation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aos.15222","type":"journal-article","created":{"date-parts":[[2022,8,3]],"date-time":"2022-08-03T10:33:40Z","timestamp":1659522820000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Effects of topical docosahexaenoic acid on postoperative fibrosis in an animal model of glaucoma filtration surgery"],"prefix":"10.1111","volume":"101","author":[{"given":"Chlo\u00e9","family":"Carr\u00e9","sequence":"first","affiliation":[{"name":"Department of Ophthalmology University Hospital  Dijon France"},{"name":"Eye and Nutrition Research Group, Centre des Sciences du Go\u00fbt et de l'Alimentation, AgroSup Dijon, CNRS, INRAE Universit\u00e9 de Bourgogne Franche\u2010Comt\u00e9  Dijon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4976-9876","authenticated-orcid":false,"given":"Florian","family":"Baudin","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology University Hospital  Dijon France"},{"name":"\u00c9quipe d'Accueil (EA 7460): Physiopathologie et \u00c9pid\u00e9miologie C\u00e9r\u00e9bro\u2010Cardiovasculaires (PEC2) Facult\u00e9 des Sciences de Sant\u00e9, Universit\u00e9 de Bourgogne\u2010Franche\u2010Comt\u00e9  Dijon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00e9n\u00e9dicte","family":"Buteau","sequence":"additional","affiliation":[{"name":"Eye and Nutrition Research Group, Centre des Sciences du Go\u00fbt et de l'Alimentation, AgroSup Dijon, CNRS, INRAE Universit\u00e9 de Bourgogne Franche\u2010Comt\u00e9  Dijon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucy","family":"Martine","sequence":"additional","affiliation":[{"name":"Eye and Nutrition Research Group, Centre des Sciences du Go\u00fbt et de l'Alimentation, AgroSup Dijon, CNRS, INRAE Universit\u00e9 de Bourgogne Franche\u2010Comt\u00e9  Dijon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phane","family":"Gr\u00e9goire","sequence":"additional","affiliation":[{"name":"Eye and Nutrition Research Group, Centre des Sciences du Go\u00fbt et de l'Alimentation, AgroSup Dijon, CNRS, INRAE Universit\u00e9 de Bourgogne Franche\u2010Comt\u00e9  Dijon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glenda","family":"Vasku","sequence":"additional","affiliation":[{"name":"Eye and Nutrition Research Group, Centre des Sciences du Go\u00fbt et de l'Alimentation, AgroSup Dijon, CNRS, INRAE Universit\u00e9 de Bourgogne Franche\u2010Comt\u00e9  Dijon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Berdeaux","sequence":"additional","affiliation":[{"name":"Eye and Nutrition Research Group, Centre des Sciences du Go\u00fbt et de l'Alimentation, AgroSup Dijon, CNRS, INRAE Universit\u00e9 de Bourgogne Franche\u2010Comt\u00e9  Dijon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnaud","family":"B\u00e9duneau","sequence":"additional","affiliation":[{"name":"PEPITE EA4267, Labex LipSTIC Bourgogne\u2010Franche\u2010Comt\u00e9 University  Besan\u00e7on France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yann","family":"Pellequer","sequence":"additional","affiliation":[{"name":"PEPITE EA4267, Labex LipSTIC Bourgogne\u2010Franche\u2010Comt\u00e9 University  Besan\u00e7on France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jasmine","family":"Jamoussi","sequence":"additional","affiliation":[{"name":"PEPITE EA4267, Labex LipSTIC Bourgogne\u2010Franche\u2010Comt\u00e9 University  Besan\u00e7on France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Desrumeaux","sequence":"additional","affiliation":[{"name":"Molecular Mechanisms in Neurodegenerative Dementia Laboratory (MMDN), INSERM, U1198 Environmental Impact in Alzheimer's Disease and Related Disorders (EiAlz) Team  Montpellier France"},{"name":"University of Montpellier  Montpellier France"},{"name":"EPHE  Paris France"},{"name":"MMDN, University of Montpellier, INSERM, EPHE  Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serge","family":"Aho","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Biostatistics University Hospital  Dijon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7265-931X","authenticated-orcid":false,"given":"Alain\u2010Marie","family":"Bron","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology University Hospital  Dijon France"},{"name":"Eye and Nutrition Research Group, Centre des Sciences du Go\u00fbt et de l'Alimentation, AgroSup Dijon, CNRS, INRAE Universit\u00e9 de Bourgogne Franche\u2010Comt\u00e9  Dijon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niyazi","family":"Acar","sequence":"additional","affiliation":[{"name":"Eye and Nutrition Research Group, Centre des Sciences du Go\u00fbt et de l'Alimentation, AgroSup Dijon, CNRS, INRAE Universit\u00e9 de Bourgogne Franche\u2010Comt\u00e9  Dijon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3587-4598","authenticated-orcid":false,"given":"Catherine","family":"Creuzot\u2010Garcher","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology University Hospital  Dijon France"},{"name":"Eye and Nutrition Research Group, Centre des Sciences du Go\u00fbt et de l'Alimentation, AgroSup Dijon, CNRS, INRAE Universit\u00e9 de Bourgogne Franche\u2010Comt\u00e9  Dijon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9688-4845","authenticated-orcid":false,"given":"Pierre Henri","family":"Gabrielle","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology University Hospital  Dijon France"},{"name":"Eye and Nutrition Research Group, Centre des Sciences du Go\u00fbt et de l'Alimentation, AgroSup Dijon, CNRS, INRAE Universit\u00e9 de Bourgogne Franche\u2010Comt\u00e9  Dijon France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,8,3]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/aos.13983"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2008.08.010"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2020.111220"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0420.1999.770108.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plefa.2009.05.010"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1089\/jop.1990.6.175"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.110.971853"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2013.03.045"},{"key":"e_1_2_8_10_1","first-page":"756","article-title":"TGF\u2010beta1, \u2212beta2, and \u2010beta3 in vitro: biphasic effects on Tenon's fibroblast contraction, proliferation, and migration","volume":"41","author":"Cordeiro M.F.","year":"2000","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"e_1_2_8_11_1","volume-title":"Effects of docosahexaenoic acid on the viability of human tenon's fibroblasts","author":"De Lazzer A.","year":"2017"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2011.03.016"},{"key":"e_1_2_8_13_1","first-page":"2020","volume-title":"Terminology and guidelines for glaucoma","author":"European Glaucoma Society","year":"2020"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)64849-5"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2020.111432"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(17)31469-1"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.1993.01090020117035"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41433-020-1031-9"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10030386"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2017.03.007"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.8.1745"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/310186"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00417-005-1170-5"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0035627"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)40190-7"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2018.01.019"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2015.08.049"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/vop.12832"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2004.06.002"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2017.08.001"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2020.100879"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/aos.12766"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.pbr.2015.05.002"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaophthalmol.2017.5702"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1097\/ICU.0000000000000729"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2013.11.025"}],"container-title":["Acta Ophthalmologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aos.15222","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/aos.15222","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aos.15222","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,20]],"date-time":"2023-08-20T13:01:11Z","timestamp":1692536471000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/aos.15222"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,3]]},"references-count":35,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,2]]}},"alternative-id":["10.1111\/aos.15222"],"URL":"https:\/\/doi.org\/10.1111\/aos.15222","archive":["Portico"],"relation":{},"ISSN":["1755-375X","1755-3768"],"issn-type":[{"value":"1755-375X","type":"print"},{"value":"1755-3768","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,3]]},"assertion":[{"value":"2022-03-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}