{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T02:08:00Z","timestamp":1779242880671,"version":"3.51.4"},"reference-count":29,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010326","name":"Stichting A.F. Deutman Oogheelkunde Researchfonds","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010326","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Ophthalmologica"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>High Magnification Module (HMM\u2122, Heidelberg Engineering, Heidelberg, Germany) imaging is a novel technique, designed to visualize the retina at a cellular level. To assess the potential of HMM\u2122\u2010based metrics as endpoints for future trials, we evaluated correlations between structural HMM\u2122 cone metrics, spectral\u2010domain OCT (SD\u2010OCT, Heidelberg Engineering, Heidelberg, Germany) and retinal sensitivity on microperimetry (MP, MAIA, CenterVue, Padova, Italy) in healthy subjects and p.(Arg142Trp) <jats:italic>PRPH2<\/jats:italic>\u2010associated Central Areolar Choroidal Dystrophy (CACD) patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We projected a default 10\u00b0 MP grid on composite HMM\u2122 images and performed automated cone density (CD), intercell distance (ICD) and nearest neighbour distance (NND) analysis at stimuli located at 3\u00b0 and 5\u00b0 retinal eccentricity. We manually measured intrasubject outer retinal thickness on SD\u2010OCT in absolute and relative scotomas, located outside of focal atrophy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We included 15 CACD patients and five healthy subjects. We found moderate\u2010to\u2010strong correlations of HMM\u2122 metrics and MP sensitivity at 3\u00b0 eccentricity from the fovea. We found the outer retina at the locations of absolute scotomas to be statistically significant thinner (<jats:italic>p<\/jats:italic>\u2009=\u20090.000003, one\u2010sample <jats:italic>t<\/jats:italic>\u2010test), as the outer retinal thickness at locations of relative scotomas. Interestingly, HMM\u2122 metrics of these areas did not differ significantly.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We found significant correlations between structural photoreceptors metrics on HMM\u2122 imaging and retinal sensitivity on MP in healthy subjects and CACD patients. A multimodal approach, combining SD\u2010OCT, MP and HMM\u2122 imaging, allows for detailed mapping of retinal photoreceptor integrity and restitution potential, important data that could serve as biomarkers in future clinical trials.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aos.15816","type":"journal-article","created":{"date-parts":[[2023,12,2]],"date-time":"2023-12-02T16:53:04Z","timestamp":1701535984000},"page":"521-528","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Structure\u2013function correlation of retinal photoreceptors in <i>PRPH2<\/i>\u2010associated central areolar choroidal dystrophy patients assessed by high\u2010resolution scanning laser imaging and microperimetry"],"prefix":"10.1111","volume":"102","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3081-9012","authenticated-orcid":false,"given":"Timo","family":"Mulders","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Donders Institute for Brain, Cognition and Behavior Radboud University Medical Center (Radboudumc)  Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ludo","family":"van der Zanden","sequence":"additional","affiliation":[{"name":"Radboud University Medical Center (Radboudumc)  Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. Jeroen","family":"Klevering","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Donders Institute for Brain, Cognition and Behavior Radboud University Medical Center (Radboudumc)  Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carel","family":"Hoyng","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Donders Institute for Brain, Cognition and Behavior Radboud University Medical Center (Radboudumc)  Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Theelen","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Donders Institute for Brain, Cognition and Behavior Radboud University Medical Center (Radboudumc)  Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,12]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/eye.2012.7"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9394(14)70212-0"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2008.12.019"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2018.08.002"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.10-6538"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.17-21-08118.1997"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.05-0997"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.visres.2011.08.008"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.17-22339"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.visres.2007.01.007"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200509036"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.18-25688"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.19-27979"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41433-019-0474-3"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/d41573-018-00016-1"},{"key":"e_1_2_8_17_1","unstructured":"Jung R.&Spillmann L.(1970)Receptive\u2010field estimation and perceptual integration in human vision. 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