{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:40:35Z","timestamp":1747183235132,"version":"3.40.5"},"reference-count":23,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,12,22]],"date-time":"2023-12-22T00:00:00Z","timestamp":1703203200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Ophthalmologica"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>This study investigated the influence of cleanroom conditions on the discard rates of donor corneas in a German university eye bank.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Discard rates were analysed from 2017 to 2020 at the LIONS Cornea Bank at Saarland University Medical Center. 1941 corneas from 971 donors were included. 1262 corneas (65.1%) were stored in a class D cleanroom from 2017 to 2019 and processed in a cleanroom class A sterile bank (group 1). 679 corneas (34.9%) were continuously stored in a class B cleanroom and processed in a class A cleanroom safety cabinet in the same room from 2019 to 2020 (group 2). The target parameter of this work was the number of contamination\u2010related discards. Although they cannot be influenced by the spatial conditions, the discards due to insufficient endothelial quality, serology, contraindications, scars and technical causes were also recorded. Statistical analysis was performed using SPSS and various testing procedures.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In group 1, significantly more corneas were discarded due to positive serology (6.9%|3.8%, <jats:italic>p<\/jats:italic>\u2009=\u20090.020). There was no significant change between both groups for either contamination or the other reasons for discard.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Optimization of hygiene standards from cleanroom class D to B did not reduce contamination. Serology, endothelial quality, medical contraindications and the presence of scars cannot be influenced by cleanroom conditions.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aos.16611","type":"journal-article","created":{"date-parts":[[2023,12,22]],"date-time":"2023-12-22T11:28:40Z","timestamp":1703244520000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Impact of cleanroom status on the reasons for discarding organ\u2010cultured corneal transplants in a modern eye bank \u2013 More donor corneas thanks to astronaut suit?"],"prefix":"10.1111","volume":"102","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-9862-852X","authenticated-orcid":false,"given":"Marco","family":"Zilles","sequence":"first","affiliation":[{"name":"Department of Ophthalmology University Hospital of the Saarland (UKS)  Homburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0423-3624","authenticated-orcid":false,"given":"Elias","family":"Flockerzi","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology University Hospital of the Saarland (UKS)  Homburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Loay","family":"Daas","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology University Hospital of the Saarland (UKS)  Homburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabel","family":"Weinstein","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology University Hospital of the Saarland (UKS)  Homburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3284-9901","authenticated-orcid":false,"given":"Loic","family":"Hamon","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology University Hospital of the Saarland (UKS)  Homburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Berthold","family":"Seitz","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology University Hospital of the Saarland (UKS)  Homburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,12,22]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10792-021-01736-x"},{"issue":"6","key":"e_1_2_7_3_1","article-title":"Beschluss der Bundes\u00e4rztekammer \u00fcber die erste umschriebene Fortschreibung der Richtlinie zur Gewinnung von Spenderhornh\u00e4uten und zum F\u00fchren einer Augenhornhautbank","volume":"115","author":"German Medical Association","year":"2018","journal-title":"Dtsch Arztebl"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaophthalmol.2020.6339"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/ICO.0b013e318222c3f2"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00347-014-3225-7"},{"key":"e_1_2_7_7_1","unstructured":"Eye Bank Association of America. 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