{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T18:15:31Z","timestamp":1779214531471,"version":"3.51.4"},"reference-count":62,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,4,4]],"date-time":"2024-04-04T00:00:00Z","timestamp":1712188800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,4,4]],"date-time":"2024-04-04T00:00:00Z","timestamp":1712188800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001349","name":"National Medical Research Council","doi-asserted-by":"publisher","award":["CNIG14NOV005"],"award-info":[{"award-number":["CNIG14NOV005"]}],"id":[{"id":"10.13039\/501100001349","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Ophthalmologica"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>To assess intra\u2010 (repeatability) and inter\u2010observer (reproducibility) variability of laser speckle flowgraphy (LSFG) for retinal blood flow (RBF) measurement in 20\u2009eyes of wild type (C57BL\/6J) mice and effect of intravitreal Aflibercept on RBF in optic nerve head (ONH) region of 10\u2009eyes of Ins2 (Akita) diabetic mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\u2018Mean blur rate (MBR)\u2019 was measured for all quadrants of tissue area (MT), vessel (MV) and total area (MA) of ONH region. Changes in MT were analysed at each timepoint. Repeatability was evaluated by measuring MBR variability without changing mouse head position, and reproducibility after resetting mouse head position by another operator. Coefficient of repeatability (CR) through Bland\u2013Altman plot method coefficient of variation (COV) and Intraclass correlation coefficient (ICC) was calculated. Intravitreal Aflibercept (1\u2009\u03bcg) was administered to Akita eyes and intraocular pressure (IOP) was measured using a tonometer at baseline, day 7, 14, 21 and 28 post\u2010injection. Hurvich and Tsai's criterion was used.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Coefficient of repeatability values of repeatability and reproducibility for all quadrants were within limits of agreement. Reliability was excellent (ICC 0.98\u20130.99) and reproducibility was moderate to excellent (ICC 0.64\u20130.96). There was a non\u2010significant IOP increase in all Akita eyes at Day 28 (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&gt;\u20090.05), and significant increase in MT in all quadrants at Day 21 and superior, inferior and temporal quadrants at Day 28 (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Laser speckle flowgraphy demonstrates excellent repeatability and moderate to excellent reproducibility in measuring RBF. Intravitreal Aflibercept injection results in a significant increase in MT up to 28\u2009days post\u2010injection without significant increase in IOP.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/aos.16672","type":"journal-article","created":{"date-parts":[[2024,4,4]],"date-time":"2024-04-04T07:35:35Z","timestamp":1712216135000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effect of\n                    <scp>anti\u2010VEGF<\/scp>\n                    on retinal blood flow in diabetic mice using laser speckle flowgraphy"],"prefix":"10.1111","volume":"102","author":[{"given":"Zheng Zhe Steven","family":"Su","sequence":"first","affiliation":[{"name":"National Healthcare Group Eye Institute Tan Tock Seng Hospital  Singapore Singapore"},{"name":"Lee Kong Chian School of Medicine Nanyang Technological University  Singapore Singapore"},{"name":"Ministry of Health Holdings  Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bryan Chin Hou","family":"Ang","sequence":"additional","affiliation":[{"name":"National Healthcare Group Eye Institute Tan Tock Seng Hospital  Singapore Singapore"},{"name":"National Healthcare Group Eye Institute Woodlands Health  Singapore Singapore"},{"name":"Ophthalmology, Mayo Clinic  Jacksonville Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Praveen Kumar","family":"Balne","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine University of Missouri  Columbia Missouri USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sai Bo Bo","family":"Tun","sequence":"additional","affiliation":[{"name":"Singapore Eye Research Institute, Singapore National Eye Centre  Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hla Myint","family":"Htoon","sequence":"additional","affiliation":[{"name":"Singapore Eye Research Institute, Singapore National Eye Centre  Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7189-1707","authenticated-orcid":false,"given":"Leopold","family":"Schmetterer","sequence":"additional","affiliation":[{"name":"Lee Kong Chian School of Medicine Nanyang Technological University  Singapore Singapore"},{"name":"Singapore Eye Research Institute, Singapore National Eye Centre  Singapore Singapore"},{"name":"Department of Ophthalmology, Yong Loo Lin School of Medicine National University of Singapore  Singapore Singapore"},{"name":"Ophthalmology and Visual Sciences Academic Clinical Program Duke\u2010NUS Medical School, National University of Singapore  Singapore Singapore"},{"name":"School of Chemistry Chemical Engineering and Biotechnology, Nanyang Technological University  Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Veluchamy Amutha","family":"Barathi","sequence":"additional","affiliation":[{"name":"Singapore Eye Research Institute, Singapore National Eye Centre  Singapore Singapore"},{"name":"Department of Ophthalmology, Yong Loo Lin School of Medicine National University of Singapore  Singapore Singapore"},{"name":"Ophthalmology and Visual Sciences Academic Clinical Program Duke\u2010NUS Medical School, National University of Singapore  Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6662-5850","authenticated-orcid":false,"given":"Rupesh","family":"Agrawal","sequence":"additional","affiliation":[{"name":"National Healthcare Group Eye Institute Tan Tock Seng Hospital  Singapore Singapore"},{"name":"Lee Kong Chian School of Medicine Nanyang Technological University  Singapore Singapore"},{"name":"Singapore Eye Research Institute, Singapore National Eye Centre  Singapore Singapore"},{"name":"Ophthalmology and Visual Sciences Academic Clinical Program Duke\u2010NUS Medical School, National University of Singapore  Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,4,4]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1089\/jop.2009.0076"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0148812"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.14\u201015373"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.2147\/OPTH.S22093"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2017.11.027"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00417\u2010008\u20100819\u20102"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvq105"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11892\u2010017\u20100909\u20109"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(86)90837-8"},{"key":"e_1_2_7_11_1","first-page":"255","article-title":"Retinal blood\u2010flow visualization by means of laser speckle photography","volume":"22","author":"Briers J.D.","year":"1982","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjophthalmol\u20102017\u2010311750"},{"issue":"10","key":"e_1_2_7_13_1","first-page":"456","article-title":"Biostatistics 301A. Repeated measurement analysis (mixed models)","volume":"45","author":"Chan Y.H.","year":"2004","journal-title":"Singapore Medical Journal"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.2337\/dc13\u20101990"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.5.000630"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0030\u20104018(81)90428\u20104"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1210\/edrv.18.1.0287"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2214\u2010109X(17)30393\u20105"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.11\u20108624"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07424"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874364101307010004"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.7.000287"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.3109\/08037051.2013.782956"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1350\u20109462(98)00037\u20108"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1159\/000099298"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00347\u2010007\u20101569\u2010y"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/852183"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2014.05.006"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1993.265.2.H586"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1097\/APO.0000000000000160"},{"issue":"8","key":"e_1_2_7_31_1","first-page":"1808","article-title":"VEGF increases retinal vascular ICAM\u20101 expression in\u00a0vivo","volume":"40","author":"Lu M.","year":"1999","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0168190"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/536161"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.2337\/dc10\u20100493"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.22.1.016005"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1097\/APO.0000000000000183"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.4103\/0301\u20104738.194329"},{"key":"e_1_2_7_38_1","doi-asserted-by":"crossref","DOI":"10.17226\/25801","volume-title":"Guide for the care and use of laboratory animals","author":"National Research Council (US) Committee for the Update of the Guide for the Care and Use of Laboratory Animals","year":"2011"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2011.12.039"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2009.04.023"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjophthalmol\u20102016\u2010310042"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.3129\/i08\u2010049"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.21037\/cdt.2017.03.12"},{"key":"e_1_2_7_44_1","volume-title":"Foundations of clinical research: applications to practice","author":"Portney L.G.","year":"2000"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2008.02.002"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755\u20103768.2009.01621.x"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2010.09.002"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.14\u201014116"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD007920.pub3"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaophthalmol.2014.3616"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnene.2010.00128"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755\u20103768.2009.01586.x"},{"issue":"10","key":"e_1_2_7_53_1","first-page":"2041","article-title":"Retinal blood flow during hyperglycemia. A laser Doppler velocimetry study","volume":"31","author":"Sullivan P.M.","year":"1990","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/627674"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajoc.2021.101123"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.61.5.27"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1549\u20108719.2010.00045.x"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.16\u201019945"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.survophthal.2018.03.003"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.12\u20109514"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0045876"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajoc.2022.101616"},{"issue":"87","key":"e_1_2_7_63_1","first-page":"44","article-title":"Anti\u2010VEGF drugs in the prevention of blindness","volume":"27","author":"Yorston D.","year":"2014","journal-title":"Community Eye Health"}],"container-title":["Acta Ophthalmologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aos.16672","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/aos.16672","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aos.16672","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T17:12:56Z","timestamp":1772989976000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/aos.16672"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,4]]},"references-count":62,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,9]]}},"alternative-id":["10.1111\/aos.16672"],"URL":"https:\/\/doi.org\/10.1111\/aos.16672","archive":["Portico"],"relation":{},"ISSN":["1755-375X","1755-3768"],"issn-type":[{"value":"1755-375X","type":"print"},{"value":"1755-3768","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,4]]},"assertion":[{"value":"2023-10-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}