{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T21:22:33Z","timestamp":1790889753279,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,3,30]],"date-time":"2024-03-30T00:00:00Z","timestamp":1711756800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100019562","name":"Synoptik-Fonden","doi-asserted-by":"publisher","award":["50.000 DKK"],"award-info":[{"award-number":["50.000 DKK"]}],"id":[{"id":"10.13039\/100019562","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Ophthalmologica"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>To compare the effect of the ocular antiseptic treatments 0.05% chlorhexidine, 5% povidone\u2010iodine (PI) and 5% betadine on cell viability and mucin secretion of primary cultured human goblet cells (GCs).<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>GC viability was analysed using lactate dehydrogenase (LDH) and tetrazolium dye (MTT) colorimetric assays. Expression of mucin was visualised by immunohistochemical MUC5AC staining.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>PI and betadine significantly reduced GC survival compared to the control (mean cell survival 23\u2009\u00b1\u20096% and 23\u2009\u00b1\u20097%, respectively, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05), whereas chlorhexidine did not significantly affect GC viability (mean cell survival: 78\u2009\u00b1\u200917%), as measured by the LDH assay. Similar results were obtained from the MTT assay, where PI and betadine caused a significant loss of GCs (mean cell survival: 26\u2009\u00b1\u200912% and 26\u2009\u00b1\u200913%, respectively, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). Chlorhexidine did not significantly alter GC survival compared to the control (mean cell survival: 79\u2009\u00b1\u20098%). PI and betadine caused a dispersion of mucin secretion, which chlorhexidine did not.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The most used antiseptic treatments, PI and betadine, applied prior to ocular surgery are significantly more cytotoxic to conjunctival GCs than chlorhexidine treatment.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aos.16684","type":"journal-article","created":{"date-parts":[[2024,3,30]],"date-time":"2024-03-30T08:39:10Z","timestamp":1711787950000},"page":"773-778","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Effect of chlorhexidine, povidone\u2010iodine and betadine antiseptic eye drops on cultured human conjunctival goblet cell survival"],"prefix":"10.1111","volume":"102","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0615-2481","authenticated-orcid":false,"given":"Rubin","family":"Hadad","sequence":"first","affiliation":[{"name":"Department of Drug Design and Pharmacology University of Copenhagen  Copenhagen E Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7556-4658","authenticated-orcid":false,"given":"Anne","family":"Hedengran","sequence":"additional","affiliation":[{"name":"Department of Drug Design and Pharmacology University of Copenhagen  Copenhagen E Denmark"},{"name":"Department of Ophthalmology Copenhagen University Hospital  Glostrup Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex","family":"Barnils","sequence":"additional","affiliation":[{"name":"Department of Drug Design and Pharmacology University of Copenhagen  Copenhagen E Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2905-9252","authenticated-orcid":false,"given":"Goran","family":"Petrovski","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Center for eye Research and Innovative Diagnostics Oslo 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