{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T09:45:41Z","timestamp":1751363141246},"reference-count":21,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,4,1]],"date-time":"2014-04-01T00:00:00Z","timestamp":1396310400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Paediatrica"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To determine the prevalence of various types of viruses in infants hospitalised due to respiratory distress, compare molecular diagnostic tests and evaluate symptom severity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>All 136 nasopharyngeal aspirates from infants hospitalised for respiratory distress over a 9\u2010month period were analysed for virus type by in\u2010house respiratory syncytial virus (<jats:styled-content style=\"fixed-case\">RSV<\/jats:styled-content>) polymerase chain reaction (<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>) microarray\u2010based and\/or Luminex\u2010based multiplex molecular tests. Medical records were reviewed retrospectively for clinical data.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Viral aetiology was confirmed in 126 subjects (92.6%) with 26 infected by more than one virus. <jats:styled-content style=\"fixed-case\">RSVA<\/jats:styled-content>\/B was the most common (50.9%), followed by entero\/rhinovirus (21.6%), human metapneumovirus (10.5%), parainfluenza virus (5.9%) and influenza (3.3%). <jats:styled-content style=\"fixed-case\">RSV<\/jats:styled-content>\u2010infected infants had significantly lower saturation levels (89% versus 92%, p\u00a0&lt;\u00a00.001), higher demand for oxygen (42.7% versus 21.6%, p\u00a0=\u00a00.021) and fluids (28% versus 9.8%; p\u00a0=\u00a00.014) and longer hospital stays (4 versus 3\u00a0days, &lt;0.001) than other viruses. Luminex assays gave repeatable, slightly less sensitive results than in\u2010house <jats:styled-content style=\"fixed-case\">RSV PCR<\/jats:styled-content>. Microarray\u2010based assays were more sensitive, however, producing some unrepeatable results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Respiratory syncytial virus dominates as the viral cause in hospitalised infants with respiratory distress in Sweden during the winter season, resulting in a clinical course that is significantly more severe. The multiplex assays produced reasonably concordant results.<\/jats:p><\/jats:sec>","DOI":"10.1111\/apa.12623","type":"journal-article","created":{"date-parts":[[2014,3,8]],"date-time":"2014-03-08T10:15:35Z","timestamp":1394273735000},"page":"625-629","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Viral aetiology and clinical outcomes in hospitalised infants presenting with respiratory distress"],"prefix":"10.1111","volume":"103","author":[{"given":"Kathy","family":"Falkenstein\u2010Hagander","sequence":"first","affiliation":[{"name":"Department of Pediatrics Sk\u00e5ne University Hospital Malm\u00f6 Lund University Malm\u00f6 Sweden"},{"name":"Department of Anesthesiology Children's Hospital Boston Boston MA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ann\u2010Sofie","family":"M\u00e5nsson","sequence":"additional","affiliation":[{"name":"Laboratory Medicine Sk\u00e5ne University Hospital Malm\u00f6 Lund University Malm\u00f6 Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johan","family":"Redmo","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases Sk\u00e5ne University Hospital Malm\u00f6 Lund University Malm\u00f6 Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Percy","family":"Nilsson Wimar","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Sk\u00e5ne University Hospital Malm\u00f6 Lund University Malm\u00f6 Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anders","family":"Widell","sequence":"additional","affiliation":[{"name":"Laboratory Medicine Sk\u00e5ne University Hospital Malm\u00f6 Lund University Malm\u00f6 Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e31822e68e6"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.2174\/187152612800100099"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s15010-002-2122-1"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/jech.2004.026450"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.inf.0000053881.47279.d9"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)69077-6"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jped.2013.02.022"},{"key":"e_1_2_8_9_1","first-page":"10401","article-title":"Epidemiology and etiology of childhood pneumonia in 2010: estimates of incidence, severe morbidity, mortality, underlying risk factors and causative pathogens for 192 countries","volume":"3","author":"Rudan I","year":"2013","journal-title":"J Glob Health"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2009.02022.x"},{"key":"e_1_2_8_11_1","first-page":"508","article-title":"Viral etiology in hospitalized children with acute lower respiratory tract infection","volume":"53","author":"Hatipoglu N","year":"2011","journal-title":"Turk J Pediatr"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-011-1300-4"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.20243"},{"key":"e_1_2_8_14_1","first-page":"S164","article-title":"Clinical and epidemiological characteristics of respiratory syncytial virus and influenza virus associated hospitalization in urban Thai infants","volume":"94","author":"Suntarattiwong P","year":"2011","journal-title":"J Med Assoc Thai"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.100.6.943"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/1824-7288-35-11"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e3181fcb159"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/1348-0421.12102"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1086\/506350"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.21108"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-200x.2005.02099.x"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3156.2011.02811.x"}],"container-title":["Acta Paediatrica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fapa.12623","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apa.12623","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T14:13:06Z","timestamp":1696515186000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/apa.12623"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,4]]},"references-count":21,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2014,6]]}},"alternative-id":["10.1111\/apa.12623"],"URL":"https:\/\/doi.org\/10.1111\/apa.12623","archive":["Portico"],"relation":{},"ISSN":["0803-5253","1651-2227"],"issn-type":[{"value":"0803-5253","type":"print"},{"value":"1651-2227","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,4]]},"assertion":[{"value":"2013-08-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-03-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}