{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T21:25:03Z","timestamp":1773264303498,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,12,20]],"date-time":"2016-12-20T00:00:00Z","timestamp":1482192000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007601","name":"Horizon 2020","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007601","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Paediatrica"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Respiratory distress syndrome (<jats:styled-content style=\"fixed-case\">RDS<\/jats:styled-content>) is a major cause of mortality and morbidity in premature infants. By the time symptoms appear, it may already be too late to prevent a severe course, with bronchopulmonary dysplasia or mortality. We aimed to develop a rapid test of lung maturity for targeting surfactant supplementation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Concentrations of the most surface\u2010active lung phospholipid dipalmitoylphosphatidylcholine and sphingomyelin in gastric aspirates from premature infants were measured by mass spectrometry and expressed as the lecithin\/sphingomyelin ratio (L\/S). The same aspirates were analysed with mid\u2010infrared spectroscopy. Subsequently, L\/S was measured in gastric aspirates and oropharyngeal secretions from another group of premature infants using spectroscopy and the results were compared with <jats:styled-content style=\"fixed-case\">RDS<\/jats:styled-content> development. The 10\u2010minute analysis required 10 \u03bcL of aspirate.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>An L\/S algorithm was developed based on 89 aspirates. Subsequently, gastric aspirates were sampled in 136 infants of 24\u201331 weeks of gestation and 61 (45%) developed <jats:styled-content style=\"fixed-case\">RDS<\/jats:styled-content>. The cut\u2010off value of L\/S was 2.2, sensitivity was 92%, and specificity was 73%. In 59 cases, the oropharyngeal secretions had less valid L\/S than gastric aspirate results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our rapid test for lung maturity, based on spectroscopy of gastric aspirate, predicted <jats:styled-content style=\"fixed-case\">RDS<\/jats:styled-content> with high sensitivity.<\/jats:p><\/jats:sec>","DOI":"10.1111\/apa.13683","type":"journal-article","created":{"date-parts":[[2016,11,25]],"date-time":"2016-11-25T16:36:29Z","timestamp":1480091789000},"page":"430-437","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Rapid test for lung maturity, based on spectroscopy of gastric aspirate, predicted respiratory distress syndrome with high sensitivity"],"prefix":"10.1111","volume":"106","author":[{"given":"Henrik","family":"Verder","sequence":"first","affiliation":[{"name":"Departments of Pediatrics Holbaek University Hospital Holbaek 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