{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T14:07:54Z","timestamp":1791986874329,"version":"4.4.0"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,5,13]],"date-time":"2020-05-13T00:00:00Z","timestamp":1589328000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Paediatrica"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Our aim was to analyse the diagnostic workup of hospitalised infants with symptoms of congenital cytomegalovirus (CMV) infections.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This retrospective study was carried out at the University Hospital Frankfurt, Germany, from 2008 to 2017 on infants aged 4\u00a0weeks to 12\u00a0months presenting with neurological symptoms consistent with congenital CMV infections.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We studied 117 infants, and workup data for CMV infections were available for 84%. Of these, 54% were immunoglobulin G\u2010 and immunoglobulin M\u2010seronegative for CMV or immunoglobulin G\u2010seropositive with no viral shedding. Congenital CMV infection was excluded in these cases. In 16%, the CMV workup was incomplete, precluding a definitive diagnosis. Dried blood spots (DBS) were requested from 30%. CMV polymerase chain reaction was negative in 19 of these 29 infants, and CMV deoxyribonucleic acid detection confirmed congenital CMV infections in six patients. DBS had been destroyed in line with German law in four cases. Congenital CMV infections were diagnosed (5%) or excluded (62%) in 67% of patients and unanswered in the remaining 33%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Diagnoses of congenital CMV infections were widely considered and found in 5%. CMV was not stringently investigated in all patients or remained elusive due to German law on destroying DBS.<\/jats:p><\/jats:sec>","DOI":"10.1111\/apa.15305","type":"journal-article","created":{"date-parts":[[2020,4,13]],"date-time":"2020-04-13T05:49:29Z","timestamp":1586756969000},"page":"197-202","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Retrospectively diagnosing congenital cytomegalovirus infections in symptomatic infants is challenging"],"prefix":"10.1111","volume":"110","author":[{"given":"Natalia","family":"L\u00fcsebrink","sequence":"first","affiliation":[{"name":"Department of Neurology, Neurometabolics and Prevention Clinic for Children and Adolescents University Hospital Frankfurt\/Main Frankfurt\/Main Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Kieslich","sequence":"additional","affiliation":[{"name":"Department of Neurology, Neurometabolics and Prevention Clinic for Children and Adolescents University Hospital Frankfurt\/Main Frankfurt\/Main Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9394-2176","authenticated-orcid":false,"given":"Holger F.","family":"Rabenau","sequence":"additional","affiliation":[{"name":"Department of Hygiene Institute for Medical Virology University Hospital Frankfurt\/Main Frankfurt\/Main Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rolf L.","family":"Schl\u00f6\u00dfer","sequence":"additional","affiliation":[{"name":"Department of Neonatology Clinic for Children and Adolescents University Hospital Frankfurt\/Main Frankfurt\/Main Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6196-9171","authenticated-orcid":false,"given":"Horst","family":"Buxmann","sequence":"additional","affiliation":[{"name":"Department of Neonatology Clinic for Children and Adolescents University Hospital Frankfurt\/Main Frankfurt\/Main Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,5,13]]},"reference":[{"key":"e_1_2_10_2_1","first-page":"45","article-title":"Primary human cytomegalovirus (HCMV) infection in pregnancy","volume":"114","author":"Buxmann H","year":"2017","journal-title":"Dtsch Arztebl Int"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2458-5-70"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2016-2128"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pcl.2012.12.008"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.544"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1404599"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpeds.2013.12.007"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2014-1173"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2018-3095"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(17)30143-3"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0000000000001763"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1136\/archdischild\u20102019\u2010317650"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12985-015-0281-9"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpeds.2017.01.047"},{"key":"e_1_2_10_16_1","unstructured":"BundesausschussG HessJR.Bekanntmachung eines Beschlusses des Gemeinsamen Bundesausschusses \u00fcber eine \u00c4nderung der Richtlinien des Bundesausschusses der \u00c4rzte und Krankenkassen \u00fcber die Fr\u00fcherkennung von Krankheiten bei Kindern bis zur Vollendung des 6. 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