{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T20:59:48Z","timestamp":1776891588315,"version":"3.51.2"},"reference-count":40,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,2,17]],"date-time":"2022-02-17T00:00:00Z","timestamp":1645056000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"publisher","award":["2019\u201001157"],"award-info":[{"award-number":["2019\u201001157"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003792","name":"Hj\u00e4rnfonden","doi-asserted-by":"publisher","award":["FO2019\u20100087"],"award-info":[{"award-number":["FO2019\u20100087"]}],"id":[{"id":"10.13039\/501100003792","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003792","name":"Hj\u00e4rnfonden","doi-asserted-by":"publisher","award":["FO2019\u20100006"],"award-info":[{"award-number":["FO2019\u20100006"]}],"id":[{"id":"10.13039\/501100003792","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Paediatrica"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Perinatal asphyxia, resulting in hypoxic\u2010ischaemic encephalopathy (HIE), has been associated with high mortality rates and severe lifelong neurodevelopmental disabilities. Our aim was to study the association between the proteomic profile in cerebrospinal fluid (CSF) and the degree of HIE and long\u2010term outcomes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We prospectively enrolled 18\u2010term born infants with HIE and 10\u2010term born controls between 2000 and 2004 from the Karolinska University Hospital. An antibody suspension bead array and FlexMap3D analysis was used to characterise 178 unique brain\u2010derived and inflammation associated proteins in their CSF.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Increased CSF concentrations of several brain\u2010specific proteins were observed in the proteome of HIE patients compared with the controls. An upregulation of neuroinflammatory pathways was also noted and this was confirmed by pathway analysis. Principal component analysis revealed a gradient from favourable to unfavourable HIE grades and outcomes. The proteins that provided strong predictors were structural proteins, including myelin basic protein and alpha\u2010II spectrin. The functional proteins included energy\u2010related proteins like neuron\u2010specific enolase and synaptic regulatory proteins. Increased CSF levels of 51 proteins correlated with adverse outcomes in infants with HIE.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Brain\u2010specific proteins and neuroinflammatory mediators in CSF may predict HIE degrees and outcomes after perinatal asphyxia.<\/jats:p><\/jats:sec>","DOI":"10.1111\/apa.16277","type":"journal-article","created":{"date-parts":[[2022,2,2]],"date-time":"2022-02-02T08:14:58Z","timestamp":1643789698000},"page":"961-970","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Proteomic profiles in cerebrospinal fluid predicted death and disability in term infants with perinatal asphyxia: A pilot study"],"prefix":"10.1111","volume":"111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0491-7302","authenticated-orcid":false,"given":"Kristin","family":"Leifsdottir","sequence":"first","affiliation":[{"name":"Department of Women\u00b4s and Children\u00b4s Health Karolinska Institutet  Stockholm Sweden"},{"name":"Astrid Lindgren Children\u2019s Hospital Karolinska University Hospital  Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2338-4364","authenticated-orcid":false,"given":"Eric P","family":"Thelin","sequence":"additional","affiliation":[{"name":"Department of Clinical Neuroscience Karolinska Institutet  Stockholm Sweden"},{"name":"Department of Neurology Karolinska University Hospital  Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0833-1093","authenticated-orcid":false,"given":"Philipp","family":"Lassar\u00e9n","sequence":"additional","affiliation":[{"name":"Department of Clinical Neuroscience Karolinska Institutet  Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Veronica","family":"Siljehav","sequence":"additional","affiliation":[{"name":"Department of Women\u00b4s and Children\u00b4s Health Karolinska Institutet  Stockholm Sweden"},{"name":"Astrid Lindgren Children\u2019s Hospital Karolinska University Hospital  Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4657-8532","authenticated-orcid":false,"given":"Peter","family":"Nilsson","sequence":"additional","affiliation":[{"name":"Department of Protein Science KTH Royal Institute of Technology SciLifeLab  Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7326-9471","authenticated-orcid":false,"given":"Staffan","family":"Eksborg","sequence":"additional","affiliation":[{"name":"Department of Women\u00b4s and Children\u00b4s Health Karolinska Institutet  Stockholm Sweden"},{"name":"Astrid Lindgren Children\u2019s Hospital Karolinska University Hospital  Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6859-0620","authenticated-orcid":false,"given":"Eric","family":"Herlenius","sequence":"additional","affiliation":[{"name":"Department of Women\u00b4s and Children\u00b4s Health Karolinska Institutet  Stockholm Sweden"},{"name":"Astrid Lindgren Children\u2019s Hospital Karolinska University Hospital  Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,2,17]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)31593-8"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/14767058.2017.1321628"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP274928"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrneurol.2015.13"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.earlhumdev.2016.12.005"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2016.08.058"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-021-03503-x"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2018.01299"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1210-1248"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1001\/archneur.1976.00500100030012"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.infbeh.2015.06.004"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-018-1253-y"},{"key":"e_1_2_11_14_1","unstructured":"Collaborators. Human protein atlas.https:\/\/www.proteinatlas.org\/Published 2021 November 18 Accessed"},{"key":"e_1_2_11_15_1","unstructured":"R Core Team. R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing.https:\/\/www.r\u2010project.org\/Published 2021 Accessed"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-4328(01)00297-2"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2019.00858"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1203\/01.PDR.0000148286.53572.95"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuint.2019.104509"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0184593"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-016-1450-y"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1212\/01.wnl.0000227183.21314.cd"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12028-013-9916-0"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1089\/neu.2010.1374"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X17724681"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2020.e03374"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clp.2008.07.012"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2017.465"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-35825-2"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1080\/14767058.2017.1304536"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2011.01.011"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1089\/neu.2006.0055"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.93.2.234"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2012.12.006"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2007.05086.x"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA119.007714"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1097\/PCC.0000000000000059"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1089\/neu.2011.1989"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-017-0821-y"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2017.10.006"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/apa.12361"}],"container-title":["Acta Paediatrica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apa.16277","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/apa.16277","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apa.16277","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T15:52:26Z","timestamp":1692805946000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/apa.16277"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,17]]},"references-count":40,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2022,5]]}},"alternative-id":["10.1111\/apa.16277"],"URL":"https:\/\/doi.org\/10.1111\/apa.16277","archive":["Portico"],"relation":{},"ISSN":["0803-5253","1651-2227"],"issn-type":[{"value":"0803-5253","type":"print"},{"value":"1651-2227","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,17]]},"assertion":[{"value":"2021-10-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-01-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-02-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}