{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T12:02:45Z","timestamp":1781092965131,"version":"3.54.1"},"reference-count":12,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:00:00Z","timestamp":1773792000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:00:00Z","timestamp":1773792000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Paediatrica"],"published-print":{"date-parts":[[2026,7]]},"abstract":"ABSTRACT<\/jats:title>\n \n Aim<\/jats:title>\n \n The aim of this study was to investigate the occurrence of\n \n M. pneumoniae<\/jats:italic>\n <\/jats:styled-content>\n detections in asymptomatic children during an outbreak.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n We prospectively recruited symptomatic and asymptomatic children under 18\u2009years of age between 1 January and 1 November 2024. Picornavirus positivity was also assessed in both groups. A multiplex polymerase chain reaction (PCR) panel was used to detect respiratory pathogens in nasopharyngeal swab samples.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n A total of 183 children with respiratory symptoms (median age 11\u2009years, IQR 7.4 to 14) and 61 asymptomatic children (median age 11\u2009months, IQR 3.0 to 121) were included. In the symptomatic group, 46 children (25%, 95% CI 19%\u201332%) tested positive for\n \n M. pneumoniae<\/jats:italic>\n <\/jats:styled-content>\n , whereas none of the asymptomatic\/mildly symptomatic children (0%, 95% CI 0%\u20136.3%) tested positive (\n p<\/jats:italic>\n \u2009<\u20090.001). Forty\u2010seven symptomatic children (26%, 95% CI 20%\u201333%) and eight asymptomatic\/mildly symptomatic children (14%, 95% CI 6.3%\u201326%) tested positive for picornaviruses (\n p<\/jats:italic>\n \u2009=\u20090.052).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n \n M. pneumoniae<\/jats:italic>\n <\/jats:styled-content>\n detections were rare in asymptomatic\/mildly symptomatic children during an outbreak. These findings suggest that multiplex PCR of nasopharyngeal swabs may serve as a specific diagnostic test for acute mycoplasma infections. The generalizability of the study's findings to school\u2010aged children is, however, limited.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/apa.70518","type":"journal-article","created":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T13:04:16Z","timestamp":1773839056000},"page":"1496-1502","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Asymptomatic\n \n Mycoplasma pneumoniae<\/i>\n <\/scp>\n Infections in Children During a Mycoplasma Outbreak"],"prefix":"10.1111","volume":"115","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8288-9442","authenticated-orcid":false,"given":"Minna","family":"Honkila","sequence":"first","affiliation":[{"name":"Department of Paediatrics and Adolescent Medicine Oulu University Hospital Oulu Finland"},{"name":"Research Unit of Clinical Medicine and Medical Research Center (MRC) Oulu University of Oulu Oulu Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ville","family":"Lindholm","sequence":"additional","affiliation":[{"name":"Department of Paediatrics and Adolescent Medicine Oulu University Hospital Oulu Finland"},{"name":"Research Unit of Clinical Medicine and Medical Research Center (MRC) Oulu University of Oulu Oulu Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna\u2010Karoliina","family":"Ojala","sequence":"additional","affiliation":[{"name":"Research Unit of Clinical Medicine and Medical Research Center (MRC) Oulu University of Oulu Oulu Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulla","family":"Koskela","sequence":"additional","affiliation":[{"name":"Department of Paediatrics and Adolescent Medicine Oulu University Hospital Oulu Finland"},{"name":"Research Unit of Clinical Medicine and Medical Research Center (MRC) Oulu University of Oulu Oulu Finland"},{"name":"Department of Anaesthesiology Oulu University Hospital Oulu Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tytti","family":"Pokka","sequence":"additional","affiliation":[{"name":"Research Unit of Clinical Medicine and Medical Research Center (MRC) Oulu University of Oulu Oulu Finland"},{"name":"Research Service Unit Oulu University Hospital Oulu Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vesa","family":"M\u00e4ki\u2010Koivisto","sequence":"additional","affiliation":[{"name":"NordLab Oulu Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1162-1059","authenticated-orcid":false,"given":"Suvi","family":"Mattila","sequence":"additional","affiliation":[{"name":"Department of Paediatrics and Adolescent Medicine Oulu University Hospital Oulu Finland"},{"name":"Research Unit of Clinical Medicine and Medical Research Center (MRC) Oulu University of Oulu Oulu Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5433-4207","authenticated-orcid":false,"given":"Terhi","family":"Ruuska\u2010Loewald","sequence":"additional","affiliation":[{"name":"Department of Paediatrics and Adolescent Medicine Oulu University Hospital Oulu Finland"},{"name":"Research Unit of Clinical Medicine and Medical Research Center (MRC) Oulu University of Oulu Oulu Finland"},{"name":"Biocenter Oulu University of Oulu Oulu Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,18]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00114-16"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268824000293"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-5247(23)00344-0"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1001444"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anai.2013.01.022"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-8-93"},{"issue":"3","key":"e_1_2_12_8_1","doi-asserted-by":"crossref","first-page":"247","DOI":"10.4067\/S0716-10182005000300005","article-title":"Portaci\u00f3n far\u00edngea de Mycoplasma Pneumoniae en ni\u00f1os Chilenos [Asymptomatic Pharyngeal Carriage of Mycoplasma pneumoniae in Chilean Children]","volume":"22","author":"Palma S. C.","year":"2005","journal-title":"Revista Chilena de Infectolog\u00eda"},{"key":"e_1_2_12_9_1","unstructured":"Finnish Institute for Health and Welfare Finnish National Infectious Diseases Register Statistical Database\u2014Cases https:\/\/sampo.thl.fi\/pivot\/prod\/fi\/ttr\/cases\/fact_ttr_cases."},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01793-19"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiaa062"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.7.2799-2803.1996"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1405870"}],"container-title":["Acta Paediatrica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apa.70518","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/apa.70518","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apa.70518","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T11:16:00Z","timestamp":1781090160000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/apa.70518"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,18]]},"references-count":12,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1111\/apa.70518"],"URL":"https:\/\/doi.org\/10.1111\/apa.70518","archive":["Portico"],"relation":{},"ISSN":["0803-5253","1651-2227"],"issn-type":[{"value":"0803-5253","type":"print"},{"value":"1651-2227","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,18]]},"assertion":[{"value":"2026-01-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}