{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T09:05:09Z","timestamp":1791104709629,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,2,18]],"date-time":"2013-02-18T00:00:00Z","timestamp":1361145600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Physiologica"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Using mice deficient in the Ca<jats:sub>V<\/jats:sub>3.1 T\u2010type Ca<jats:sup>2+<\/jats:sup>channel, the aim of the present study was to elucidate the molecular identity of non\u2010L\u2010type channels involved in vascular tone regulation in mesenteric arteries and arterioles.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We used immunofluorescence microscopy to localize Ca<jats:sub>V<\/jats:sub>3.1 channels, patch clamp electrophysiology to test the effects of a putative T\u2010type channel blocker NNC\u00a055\u20100396 on whole\u2010cell Ca<jats:sup>2+<\/jats:sup>currents, pressure myography and Ca<jats:sup>2+<\/jats:sup>imaging to test diameter and Ca<jats:sup>2+<\/jats:sup>responses of the applied vasoconstrictors, and Q\u2010PCR to check<jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>expression levels of several Ca<jats:sup>2+<\/jats:sup>handling proteins in wild\u2010type and Ca<jats:sub>V<\/jats:sub>3.1<jats:sup>\u2212\/\u2212<\/jats:sup>mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our data indicated that Ca<jats:sub>V<\/jats:sub>3.1 channels are important for the maintenance of myogenic tone at low pressures (40\u201380\u00a0mm\u00a0Hg), whereas they are not involved in high\u2010voltage\u2010activated Ca<jats:sup>2+<\/jats:sup>currents, Ca<jats:sup>2+<\/jats:sup>entry or vasoconstriction to high<jats:styled-content style=\"fixed-case\">KC<\/jats:styled-content>l in mesenteric arteries and arterioles. Furthermore, we show that<jats:styled-content style=\"fixed-case\">NNC<\/jats:styled-content>\u00a055\u20130396 is not a specific T\u2010type channel inhibitor, as it potently blocks L\u2010type and non\u2010L\u2010type high\u2010voltage\u2010activated Ca<jats:sup>2+<\/jats:sup>currents in mouse mesenteric vascular smooth muscle cell.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our data using mice deficient in the Ca<jats:sub>V<\/jats:sub>3.1 T\u2010type channel represent new evidence for the involvement of non\u2010L\u2010type channels in arteriolar tone regulation. We showed that Ca<jats:sub>V<\/jats:sub>3.1 channels are important for the myogenic tone at low arterial pressure, which is potentially relevant under resting conditions<jats:italic>in vivo<\/jats:italic>. Moreover, Ca<jats:sub>V<\/jats:sub>3.1 channels are not involved in Ca<jats:sup>2+<\/jats:sup>entry and vasoconstriction to large depolarization with, for example, high<jats:styled-content style=\"fixed-case\">KC<\/jats:styled-content>l. Finally, we caution against using<jats:styled-content style=\"fixed-case\">NNC<\/jats:styled-content>55\u20130396 as a specific T\u2010type channel blocker in native cells expressing high\u2010voltage\u2010activated Ca<jats:sup>2+<\/jats:sup>channels.<\/jats:p><\/jats:sec>","DOI":"10.1111\/apha.12066","type":"journal-article","created":{"date-parts":[[2013,1,29]],"date-time":"2013-01-29T12:47:12Z","timestamp":1359463632000},"page":"709-720","source":"Crossref","is-referenced-by-count":47,"title":["Myogenic tone is impaired at low arterial pressure in mice deficient in the low\u2010voltage\u2010activated<scp>C<\/scp>a<sub>V<\/sub>3.1<scp>T<\/scp>\u2010type<scp>C<\/scp>a<sup>2+<\/sup>channel"],"prefix":"10.1111","volume":"207","author":[{"given":"K.","family":"Bj\u00f6rling","sequence":"first","affiliation":[{"name":"Department of Veterinary Clinical and Animal Sciences Faculty of Health and Medical Sciences University of Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Morita","sequence":"additional","affiliation":[{"name":"Special Patient Oral Care Unit Kyushu University Hospital Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. F.","family":"Olsen","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical and Animal Sciences Faculty of Health and Medical Sciences University of Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Prodan","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical and Animal Sciences Faculty of Health and Medical Sciences University of Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. B.","family":"Hansen","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular and Renal Research Institute of Molecular Medicine University of Southern Denmark Odense Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Lory","sequence":"additional","affiliation":[{"name":"CNRS Institut de G\u00e9nomique Fonctionnelle Universit\u00e9 de Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.\u2010H.","family":"Holstein\u2010Rathlou","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences Faculty of Health and Medical Sciences University of Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. 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