{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T13:18:50Z","timestamp":1740143930013,"version":"3.37.3"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,1,17]],"date-time":"2015-01-17T00:00:00Z","timestamp":1421452800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004063","name":"Knut and Alice Wallenberg Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004063","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Ragnar S\u00c3\u00b6derbergs Foundation"},{"name":"The Swedish Diabetes Fund"},{"DOI":"10.13039\/501100001862","name":"The Swedish Research Council","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001862","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Physiologica"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Myeloid cells have been suggested to participate in angiogenesis and regulation of vascular function. <jats:italic>Shb<\/jats:italic>\u2010deficient mice display both vascular and myeloid cell abnormalities with possible consequences for recovery after hindlimb ischaemia. This study was conducted in order to assess the contribution of <jats:italic>Shb<\/jats:italic> deficiency in myeloid cells to impaired vascular function in ischaemia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Wild type and <jats:italic>Shb<\/jats:italic>\u2010deficient mice were subjected to peritoneal <jats:styled-content style=\"fixed-case\">vascular endothelial growth factor A (VEGFA)<\/jats:styled-content> followed by intraperitoneal lavage, after which blood and peritoneal cells were stained for myeloid markers. <jats:styled-content style=\"fixed-case\">VEGFA<\/jats:styled-content>\u2010induced leucocyte recruitment to cremaster muscle was investigated using intravital microscopy of both mouse strains. Blood flow after femoral artery ligation was determined on chimeric mice after bone marrow transplantation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>No differences in neutrophil numbers or cell surface phenotypes were detected. Moreover, neutrophil extravasation in <jats:styled-content style=\"fixed-case\">VEGFA<\/jats:styled-content>\u2010activated cremaster muscle was unaffected by <jats:italic>Shb<\/jats:italic> deficiency. However, blood and peritoneal <jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>4+ monocytes\/macrophages were reduced in response to intraperitoneal <jats:styled-content style=\"fixed-case\">VEGFA<\/jats:styled-content> but not lipopolysaccharide (<jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>) in the absence of <jats:italic>Shb<\/jats:italic>. Furthermore, the macrophage population in ischaemic muscle was unaffected by <jats:italic>Shb<\/jats:italic> deficiency after 2\u00a0days but reduced 7\u00a0days after injury. The bone marrow transplantation experiments revealed that mice with wild type vasculature showed better blood flow than those with <jats:italic>Shb<\/jats:italic>\u2010deficient vasculature irrespective of leucocyte genotype.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The observed aberrations in myeloid cell properties in <jats:italic>Shb<\/jats:italic>\u2010deficient mice are likely consequences of an abnormal vascular compartment and are not responsible for reduced muscle blood flow. Structural vascular abnormalities seem to be the primary cause of poor vascular performance under provoked vascular stress in this genetic model.<\/jats:p><\/jats:sec>","DOI":"10.1111\/apha.12448","type":"journal-article","created":{"date-parts":[[2015,1,5]],"date-time":"2015-01-05T14:15:50Z","timestamp":1420467350000},"page":"200-209","source":"Crossref","is-referenced-by-count":11,"title":["<i>Shb<\/i> deficiency in endothelium but not in leucocytes is responsible for impaired vascular performance during hindlimb ischaemia"],"prefix":"10.1111","volume":"214","author":[{"given":"M.","family":"Nikpour","sequence":"first","affiliation":[{"name":"Department of Medical Cell Biology Uppsala University Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Gustafsson","sequence":"additional","affiliation":[{"name":"Department of Medical Cell Biology Uppsala University Uppsala 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