{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T04:36:11Z","timestamp":1769574971121,"version":"3.49.0"},"reference-count":23,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,8,31]],"date-time":"2015-08-31T00:00:00Z","timestamp":1440979200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Physiologica"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Visceral adipose tissue\u2010derived serine protease inhibitor (vaspin) is an adipocytokine with insulin\u2010sensitizing activity originally identified in visceral adipose tissues of obesity\u2010related type<jats:styled-content style=\"fixed-case\">II<\/jats:styled-content>diabetic rats. We previously showed that vaspin inhibits vascular cell migration and apoptosis as well as inflammatory responses, which are crucial for the development of hypertension. However, little is known about the effects of vaspin on vascular reactivity. The aim of this study was thus to explore the effects of vaspin on contraction and relaxation of isolated blood vessel.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>After mesenteric arteries were isolated from male Wistar rats, the effects of pretreatment with vaspin (3\u00a0ng\u00a0mL<jats:sup>\u22121<\/jats:sup>, 30\u00a0min) on concentration\u2013contraction and concentration\u2013relaxation relationships for each agent were examined. The effects of vaspin on acetylcholine (<jats:styled-content style=\"fixed-case\">AC<\/jats:styled-content>h)\u2010induced phosphorylation of endothelial nitric oxide (<jats:styled-content style=\"fixed-case\">NO<\/jats:styled-content>) synthase (e<jats:styled-content style=\"fixed-case\">NOS<\/jats:styled-content>) and<jats:styled-content style=\"fixed-case\">AC<\/jats:styled-content>h esterase (<jats:styled-content style=\"fixed-case\">AC<\/jats:styled-content>hE) activity were also examined using Western blotting and colorimetric method respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Vaspin did not affect noradrenaline\u2010 or 5\u2010hydroxytryptamine\u2010induced contraction. In contrast, vaspin augmented<jats:styled-content style=\"fixed-case\">AC<\/jats:styled-content>h\u2010 but not histamine\u2010, A23187\u2010 or carbachol\u2010induced<jats:styled-content style=\"fixed-case\">NO<\/jats:styled-content>\u2010mediated relaxation. Vaspin significantly increased<jats:styled-content style=\"fixed-case\">AC<\/jats:styled-content>h\u2010induced e<jats:styled-content style=\"fixed-case\">NOS<\/jats:styled-content>phosphorylation and inhibited<jats:styled-content style=\"fixed-case\">AC<\/jats:styled-content>hE activity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We for the first time demonstrate that vaspin augments the<jats:styled-content style=\"fixed-case\">AC<\/jats:styled-content>h\u2010induced<jats:styled-content style=\"fixed-case\">NO<\/jats:styled-content>\u2010mediated endothelium\u2010dependent relaxation via the inhibition of<jats:styled-content style=\"fixed-case\">AC<\/jats:styled-content>hE activity.<\/jats:p><\/jats:sec>","DOI":"10.1111\/apha.12563","type":"journal-article","created":{"date-parts":[[2015,8,12]],"date-time":"2015-08-12T08:25:57Z","timestamp":1439367957000},"page":"203-210","source":"Crossref","is-referenced-by-count":15,"title":["Visceral adipose 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