{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T18:58:17Z","timestamp":1791917897510,"version":"4.3.4"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,10,22]],"date-time":"2015-10-22T00:00:00Z","timestamp":1445472000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Department of Molecular Medicine, University of Greifswald"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Physiologica"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>The renal pelvis shows spontaneous rhythmic contractile activity. We assessed to what extent this activity depends on renal innervation and studied the role of connexins in pelvic contractions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Rats underwent unilateral renal denervation or renal transplantation. Renal pelvic pressure and diuresis were measured<jats:italic>in\u00a0vivo<\/jats:italic>. Spontaneous and agonist\u2010induced contractions of isolated renal pelves were investigated by wire myography. Rat and human renal pelvic connexin<jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>abundances and connexin localization were studied by real\u2010time<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>and immunofluorescence respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Renal denervation or transplantation increased renal pelvic pressure<jats:italic>in\u00a0vivo<\/jats:italic>by about 60 and 150%, respectively, but did not significantly affect pelvic contraction frequency. Under<jats:italic>in\u00a0vitro<\/jats:italic>conditions, isolated pelvic preparations from innervated or denervated kidneys showed spontaneous contractions. Pelves from denervated kidneys showed about 50% higher contraction frequencies than pelves from innervated kidneys, whereas contraction force was similar in pelves from denervated and innervated kidneys. There was no denervation\u2010induced supersensitivity to noradrenaline or endothelin\u20101. Renal denervation did not increase pelvic connexin37, 40, 43 or 45<jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>abundances. Gap junction blockade had no effect on spontaneous pelvic contractile activity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The denervation\u2010induced effect on pelvic pressure may be the consequence of the enhanced diuresis. The mechanisms underlying the denervation\u2010induced effects on pelvic contraction frequency remain unknown. Our data rule out a major role for two important candidates, by showing that renal denervation neither induced supersensitivity to contractile agonists nor increased connexin<jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>abundance in the pelvic wall.<\/jats:p><\/jats:sec>","DOI":"10.1111\/apha.12612","type":"journal-article","created":{"date-parts":[[2015,10,5]],"date-time":"2015-10-05T14:08:44Z","timestamp":1444054124000},"page":"240-253","source":"Crossref","is-referenced-by-count":3,"title":["Effects of renal denervation on renal pelvic contractions and connexin expression in rats"],"prefix":"10.1111","volume":"216","author":[{"given":"A.","family":"Koenen","sequence":"first","affiliation":[{"name":"Department of Physiology University of Greifswald Karlsburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Steinbach","sequence":"additional","affiliation":[{"name":"Department of Physiology University of Greifswald Karlsburg 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