{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T12:52:02Z","timestamp":1780404722331,"version":"3.54.1"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,5,25]],"date-time":"2016-05-25T00:00:00Z","timestamp":1464134400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Physiologica"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>The molecular interactions between transient receptor potential vanilloid subtype 4 channels (<jats:styled-content style=\"fixed-case\">TRPV<\/jats:styled-content>4) and cell junction formation were investigated in the human and mouse urogenital tract.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>A qualitative study was performed to investigate <jats:styled-content style=\"fixed-case\">TRPV<\/jats:styled-content>4 channels, adherence junctions (<jats:styled-content style=\"fixed-case\">AJ<\/jats:styled-content>s) and tight junctions (<jats:styled-content style=\"fixed-case\">TJ<\/jats:styled-content>s) in kidney, ureter and bladder tissues from humans and wild\u2010type and transgenic <jats:styled-content style=\"fixed-case\">TRPV<\/jats:styled-content>4 knockout (\u2212\/\u2212) mice with immunohistochemistry, Western blotting, immunoprecipitation and reverse trasnscription\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. Cell junction formation in the wild\u2010type and <jats:styled-content style=\"fixed-case\">TRPV<\/jats:styled-content>4 knockout (\u2212\/\u2212) mouse was evaluated with immunohistochemistry and transmission electron microscope (<jats:styled-content style=\"fixed-case\">TEM<\/jats:styled-content>) techniques.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">TRPV<\/jats:styled-content>4 channels are predominantly located in membranes of epithelial cells of the bladder, ureter and the collecting ducts of the kidney. There is a molecular interaction between the <jats:styled-content style=\"fixed-case\">TRPV<\/jats:styled-content>4 channel and the <jats:styled-content style=\"fixed-case\">AJ<\/jats:styled-content>. <jats:styled-content style=\"fixed-case\">TEM<\/jats:styled-content> evaluation showed that <jats:styled-content style=\"fixed-case\">AJ<\/jats:styled-content> formation is disrupted in the <jats:styled-content style=\"fixed-case\">TRPV<\/jats:styled-content>4 \u2212\/\u2212 mouse resulting in deficient intercellular connections and integrity of the epithelium.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">TRPV<\/jats:styled-content>4 is believed to be a mechanoreceptor in the bladder. This study demonstrates that <jats:styled-content style=\"fixed-case\">TRPV<\/jats:styled-content>4 is also involved in intercellular connectivity and structural integrity of the epithelium.<\/jats:p><\/jats:sec>","DOI":"10.1111\/apha.12701","type":"journal-article","created":{"date-parts":[[2016,4,28]],"date-time":"2016-04-28T14:29:55Z","timestamp":1461853795000},"page":"38-48","source":"Crossref","is-referenced-by-count":28,"title":["<scp>TRPV<\/scp>4 channels in the human urogenital tract play a role in cell junction formation and epithelial barrier"],"prefix":"10.1111","volume":"218","author":[{"given":"D. A. W.","family":"Janssen","sequence":"first","affiliation":[{"name":"Department of Urology Radboud University Nijmegen Medical Center Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. J. 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