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Besides L\u2010type channels, T\u2010 and P\/Q\u2010type calcium channels are involved in the contraction of human renal blood vessels. It was hypothesized that T\u2010 and P\/Q\u2010type channels are involved in the contraction of human brain and mammary blood vessels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Internal mammary arteries from bypass surgery patients and cerebral arterioles from patients with brain tumours with and without hypertension were tested in a myograph and perfusion set\u2010up.<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>and immunohistochemistry were performed on isolated blood vessels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The P\/Q\u2010type antagonist \u03c9\u2010agatoxin<jats:styled-content style=\"fixed-case\">IVA<\/jats:styled-content>(10<jats:sup>\u22128<\/jats:sup>\u00a0mol\u00a0L<jats:sup>\u22121<\/jats:sup>) and the T\u2010type calcium blocker mibefradil (10<jats:sup>\u22127<\/jats:sup>\u00a0mol\u00a0L<jats:sup>\u22121<\/jats:sup>) inhibited<jats:styled-content style=\"fixed-case\">KC<\/jats:styled-content>l depolarization\u2010induced contraction in mammary arteries from hypertensive patients with no effect on blood vessels from normotensive patients. \u03c9\u2010Agatoxin<jats:styled-content style=\"fixed-case\">IVA<\/jats:styled-content>decreased contraction in cerebral arterioles from hypertensive patients. L\u2010type blocker nifedipine abolished the contraction in mammary arteries.<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>analysis showed expression of P\/Q\u2010type (Ca<jats:sub>v<\/jats:sub>2.1), T\u2010type (Ca<jats:sub>v<\/jats:sub>3.1 and Ca<jats:sub>v<\/jats:sub>3.2) and L\u2010type (Ca<jats:sub>v<\/jats:sub>1.2) calcium channels in mammary and cerebral arteries. Immunohistochemical labelling of mammary and cerebral arteries revealed the presence of Ca<jats:sub>v<\/jats:sub>2.1 in endothelial and smooth muscle cells. Ca<jats:sub>v<\/jats:sub>3.1 was also detected in mammary arteries.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>P\/Q\u2010 and T\u2010type Ca<jats:sub>v<\/jats:sub>are present in human internal mammary arteries and in cerebral penetrating arterioles. P\/Q\u2010 and T\u2010type calcium channels are involved in the contraction of mammary arteries from hypertensive patients but not from normotensive patients. Furthermore, in cerebral arterioles P\/Q\u2010type channels importance was restricted to hypertensive patients might lead to that T\u2010 and P\/Q\u2010type channels could be a new target in hypertensive patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/apha.12732","type":"journal-article","created":{"date-parts":[[2016,6,7]],"date-time":"2016-06-07T15:17:37Z","timestamp":1465312657000},"page":"642-653","source":"Crossref","is-referenced-by-count":11,"title":["P\/Q\u2010type and T\u2010type voltage\u2010gated calcium channels are involved in the contraction of mammary and brain blood vessels from hypertensive patients"],"prefix":"10.1111","volume":"219","author":[{"given":"A. D.","family":"Thuesen","sequence":"first","affiliation":[{"name":"Department of Cardiovascular and Renal Research Institute of Molecular Medicine University of Southern Denmark Odense Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. 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