{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T22:51:54Z","timestamp":1790549514913,"version":"4.1.0"},"reference-count":20,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,11,30]],"date-time":"2016-11-30T00:00:00Z","timestamp":1480464000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"G\u00f6sta Fraenckel Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Physiologica"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Experiments have indicated that skin perfusion in mice is sensitive to reductions in environmental O<jats:sub>2<\/jats:sub> availability. Specifically, a reduction in skin\u2010surface <jats:styled-content style=\"fixed-case\">PO<\/jats:styled-content><jats:sub>2<\/jats:sub> attenuates transcutaneous O<jats:sub>2<\/jats:sub> diffusion, and hence epidermal O<jats:sub>2<\/jats:sub> supply. In response, epidermal <jats:styled-content style=\"fixed-case\">HIF<\/jats:styled-content>\u20101<jats:italic>\u03b1<\/jats:italic> expression increases and facilitates initial cutaneous vasoconstriction and subsequent nitric oxide\u2010dependent vasodilation. Here, we investigated whether the same mechanism exists in humans.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In a first experiment, eight males rested twice for 8\u00a0h in a hypobaric chamber. Once, barometric pressure was reduced by 50%, while systemic oxygenation was preserved by O<jats:sub>2<\/jats:sub>\u2010enriched (42%) breathing gas (Hypoxia<jats:sub>Skin<\/jats:sub>), and once barometric pressure and inspired O<jats:sub>2<\/jats:sub> fraction were normal (Control<jats:sub>1<\/jats:sub>). In a second experiment, nine males rested for 8\u00a0h with both forearms wrapped in plastic bags. O<jats:sub>2<\/jats:sub> was expelled from one bag by nitrogen flushing (Anoxia<jats:sub>Skin<\/jats:sub>), whereas the other bag was flushed with air (Control<jats:sub>2<\/jats:sub>). In both experiments, skin blood flux was assessed by laser Doppler on the dorsal forearm, and <jats:styled-content style=\"fixed-case\">HIF<\/jats:styled-content>\u20101<jats:italic>\u03b1<\/jats:italic> expression was determined by immunohistochemical staining in forearm skin biopsies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Skin blood flux during Hypoxia<jats:sub>Skin<\/jats:sub> and Anoxia<jats:sub>Skin<\/jats:sub> remained similar to the corresponding Control trial (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.67 and <jats:italic>P<\/jats:italic>\u00a0=\u00a00.81). Immunohistochemically stained epidermal <jats:styled-content style=\"fixed-case\">HIF<\/jats:styled-content>\u20101<jats:italic>\u03b1<\/jats:italic> was detected on 8.2\u00a0\u00b1\u00a06.1 and 5.3\u00a0\u00b1\u00a05.7% of the analysed area during Hypoxia<jats:sub>Skin<\/jats:sub> and Control<jats:sub>1<\/jats:sub> (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.30) and on 2.3\u00a0\u00b1\u00a01.8 and 2.4\u00a0\u00b1\u00a01.8% during Anoxia<jats:sub>Skin<\/jats:sub> and Control<jats:sub>2<\/jats:sub> (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.90) respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Reductions in skin\u2010surface <jats:styled-content style=\"fixed-case\">PO<\/jats:styled-content><jats:sub>2<\/jats:sub> do not affect skin perfusion in humans. The unchanged epidermal <jats:styled-content style=\"fixed-case\">HIF<\/jats:styled-content>\u20101<jats:italic>\u03b1<\/jats:italic> expression suggests that epidermal O<jats:sub>2<\/jats:sub> homoeostasis was not disturbed by Hypoxia<jats:sub>Skin<\/jats:sub>\/Anoxia<jats:sub>Skin<\/jats:sub>, potentially due to compensatory increases in arterial O<jats:sub>2<\/jats:sub> extraction.<\/jats:p><\/jats:sec>","DOI":"10.1111\/apha.12825","type":"journal-article","created":{"date-parts":[[2016,11,3]],"date-time":"2016-11-03T15:23:22Z","timestamp":1478186602000},"page":"361-369","source":"Crossref","is-referenced-by-count":10,"title":["Cutaneous exposure to hypoxia does not affect skin perfusion in humans"],"prefix":"10.1111","volume":"220","author":[{"given":"C.","family":"Siebenmann","sequence":"first","affiliation":[{"name":"Department of Environmental Physiology School of Technology and Health Royal Institute of Technology Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. E.","family":"Keramidas","sequence":"additional","affiliation":[{"name":"Department of Environmental Physiology School of Technology and Health Royal Institute of Technology Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Rundqvist","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology Karolinska Institutet Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Mijwel","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pharmacology Karolinska Institutet Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. S.","family":"Cowburn","sequence":"additional","affiliation":[{"name":"Departments of Physiology, Development and Neuroscience and Medicine University of Cambridge Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. S.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology Karolinska Institutet Stockholm Sweden"},{"name":"Departments of Physiology, Development and Neuroscience and Medicine University of Cambridge Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O.","family":"Eiken","sequence":"additional","affiliation":[{"name":"Department of Environmental Physiology School of Technology and Health Royal Institute of Technology Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,11,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.02.038"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.0549v.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1306942110"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtherbio.2014.09.003"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.03.044"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.221381098"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1999.87.6.2218"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.30.18709"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4419-8997-0_18"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2006.12.029"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.3109\/03091909009009955"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0047116"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.11-191692"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/1746-1596-7-42"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.01443.2006"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2000.00911.x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2001.013067"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365518409083817"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2001.00613.x"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1993.74.5.2566"}],"container-title":["Acta Physiologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fapha.12825","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fapha.12825","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apha.12825","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T03:12:43Z","timestamp":1696475563000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/apha.12825"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,11,30]]},"references-count":20,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2017,7]]}},"alternative-id":["10.1111\/apha.12825"],"URL":"https:\/\/doi.org\/10.1111\/apha.12825","archive":["Portico"],"relation":{},"ISSN":["1748-1708","1748-1716"],"issn-type":[{"value":"1748-1708","type":"print"},{"value":"1748-1716","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,11,30]]}}}