{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T21:14:02Z","timestamp":1790025242721,"version":"4.0.1"},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,2,28]],"date-time":"2018-02-28T00:00:00Z","timestamp":1519776000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100011199","name":"FP7 Ideas: European Research Council","doi-asserted-by":"publisher","award":["ICEBERG, ERC\u20102009\u2010AdG233285"],"award-info":[{"award-number":["ICEBERG, ERC\u20102009\u2010AdG233285"]}],"id":[{"id":"10.13039\/100011199","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"publisher","award":["2011\u20103550"],"award-info":[{"award-number":["2011\u20103550"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004348","name":"Stockholms L\u00e4ns Landsting","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004348","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004047","name":"Karolinska Institutet","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004047","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005366","name":"Universitetet i Oslo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005366","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Physiologica"],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Spinal cord injury\u2010induced loss of skeletal muscle mass does not progress linearly. In humans, peak muscle loss occurs during the first 6 weeks postinjury, and gradually continues thereafter. The aim of this study was to delineate the regulatory events underlying skeletal muscle atrophy during the first year following spinal cord injury.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Key translational, autophagic and proteolytic proteins were analysed by immunoblotting of human <jats:italic>vastus lateralis<\/jats:italic> muscle obtained 1, 3 and 12 months following spinal cord injury. Age\u2010matched able\u2010bodied control subjects were also studied.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Several downstream targets of Akt signalling decreased after spinal cord injury in skeletal muscle, without changes in resting Akt Ser<jats:sup>473<\/jats:sup> and Akt Thr<jats:sup>308<\/jats:sup> phosphorylation or total Akt protein. Abundance of <jats:styled-content style=\"fixed-case\">mTOR<\/jats:styled-content> protein and <jats:styled-content style=\"fixed-case\">mTOR<\/jats:styled-content> Ser<jats:sup>2448<\/jats:sup> phosphorylation, as well as <jats:styled-content style=\"fixed-case\">FOXO<\/jats:styled-content>1 Ser<jats:sup>256<\/jats:sup> phosphorylation and <jats:styled-content style=\"fixed-case\">FOXO<\/jats:styled-content>3 protein, decreased in response to spinal cord injury, coincident with attenuated protein abundance of E3 ubiquitin ligases, Mu<jats:styled-content style=\"fixed-case\">RF<\/jats:styled-content>1 and <jats:styled-content style=\"fixed-case\">MAF<\/jats:styled-content>bx. S6 protein and Ser<jats:sup>235\/236<\/jats:sup> phosphorylation, as well as 4E\u2010<jats:styled-content style=\"fixed-case\">BP<\/jats:styled-content>1 Thr<jats:sup>37\/46<\/jats:sup> phosphorylation, increased transiently after spinal cord injury, indicating higher levels of protein translation early after injury. Protein abundance of <jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>3\u2010I and <jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>3\u2010<jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> decreased 3 months postinjury as compared with 1 month postinjury, but not compared to able\u2010bodied control subjects, indicating lower levels of autophagy. Proteins regulating proteasomal degradation were stably increased in response to spinal cord injury.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Together, these data provide indirect evidence suggesting that protein translation and autophagy transiently increase, while whole proteolysis remains stably higher in skeletal muscle within the first year after spinal cord injury.<\/jats:p><\/jats:sec>","DOI":"10.1111\/apha.13051","type":"journal-article","created":{"date-parts":[[2018,2,9]],"date-time":"2018-02-09T01:53:13Z","timestamp":1518141193000},"source":"Crossref","is-referenced-by-count":21,"title":["Protein translation, proteolysis and autophagy in human skeletal muscle atrophy after spinal cord injury"],"prefix":"10.1111","volume":"223","author":[{"given":"L. S.","family":"Lundell","sequence":"first","affiliation":[{"name":"Department of Physiology and Pharmacology Section for Integrative Physiology Karolinska Institutet Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Savikj","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pharmacology Section for Integrative Physiology Karolinska Institutet Stockholm Sweden"},{"name":"Faculty of Medicine University of Oslo Oslo Norway"},{"name":"Department of Research Sunnaas Rehabilitation Hospital Nesoddtangen Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Kostovski","sequence":"additional","affiliation":[{"name":"Faculty of Medicine University of Oslo Oslo Norway"},{"name":"Department of Research Sunnaas Rehabilitation Hospital Nesoddtangen Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. O.","family":"Iversen","sequence":"additional","affiliation":[{"name":"Department of Nutrition Institute of Basic Medical Sciences University of Oslo Oslo Norway"},{"name":"Department of Hematology Oslo University Hospital Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. R.","family":"Zierath","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pharmacology Section for Integrative Physiology Karolinska Institutet Stockholm Sweden"},{"name":"Department of Molecular Medicine and Surgery Section for Integrative Physiology Karolinska Institutet Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Krook","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pharmacology Section for Integrative Physiology Karolinska Institutet Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. V.","family":"Chibalin","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine and Surgery Section for Integrative Physiology Karolinska Institutet Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"U.","family":"Widegren","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine and Surgery Section for Integrative Physiology Karolinska Institutet Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,2,28]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/10790268.2001.11753584"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1179\/2045772314Y.0000000245"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1999.86.1.350"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.21391"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1996.271.3.E593"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.sc.3101652"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.2000.88.4.1310"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)38923-9"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2672"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00736.2007"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.21293"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms7670"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1065874"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-008-8090-6"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3160065"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.23423"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1179\/2045772312Y.0000000061"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.sc.3100844"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.54.6.1692"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00686.2010"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00079.2015"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2007.11.001"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2010.3419"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-009-0643-5"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2006.118042"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3070631"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1357-2725(03)00004-9"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1177\/153537020623100315"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.4161\/auto.4600"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2010.01.017"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2069"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1080\/10790268.2016.1229397"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.12.15.1701"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00132.2013"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1179\/204577211X13207446293695"}],"container-title":["Acta Physiologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fapha.13051","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apha.13051","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T08:07:47Z","timestamp":1695456467000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/apha.13051"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,2,28]]},"references-count":35,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2018,7]]}},"alternative-id":["10.1111\/apha.13051"],"URL":"https:\/\/doi.org\/10.1111\/apha.13051","archive":["Portico"],"relation":{},"ISSN":["1748-1708","1748-1716"],"issn-type":[{"value":"1748-1708","type":"print"},{"value":"1748-1716","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,2,28]]},"article-number":"e13051"}}