{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:26:27Z","timestamp":1753878387658,"version":"3.41.2"},"reference-count":38,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,6,17]],"date-time":"2018-06-17T00:00:00Z","timestamp":1529193600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100011102","name":"Seventh Framework Programme","doi-asserted-by":"publisher","award":["606998"],"award-info":[{"award-number":["606998"]}],"id":[{"id":"10.13039\/100011102","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Physiologica"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Conduction of vasomotor responses may contribute to long\u2010term regulation of resistance artery function and structure. Most previous studies have addressed conduction of vasoactivity only during very brief stimulations. We developed a novel set\u2010up that allows the local pharmacological stimulation of arteries in\u00a0vitro for extended periods of time and studied the conduction of vasomotor responses in rat mesenteric arteries under those conditions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The new in\u00a0vitro set\u2010up was based on the pressure myograph. The superfusion chamber was divided halfway along the vessel into two compartments, allowing an independent superfusion of the arterial segment in each compartment. Local and remote cumulative concentration\u2010response curves were obtained for a range of vasoactive agents. Additional experiments were performed with the gap junction inhibitor 18\u03b2\u2010glycyrrhetinic acid and in absence of the endothelium.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Phenylephrine\u2010induced constriction and acetylcholine\u2010induced dilation were conducted over a measured distance up to 2.84\u00a0mm, and this conduction was maintained for 5\u00a0minutes. Conduction of acetylcholine\u2010induced dilation was inhibited by 18\u03b2\u2010glycyrrhetinic acid, and conduction of phenylephrine\u2010induced constriction was abolished in absence of the endothelium. Constriction in response to high K<jats:sup>+<\/jats:sup> was not conducted. Absence of remote stimulation dampened the local response to phenylephrine.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study demonstrates maintained conduction of vasoactive responses to physiological agonists in rat mesenteric small arteries likely via gap junctions and endothelial cells, providing a possible mechanism for the sustained functional and structural control of arterial networks.<\/jats:p><\/jats:sec>","DOI":"10.1111\/apha.13099","type":"journal-article","created":{"date-parts":[[2018,5,21]],"date-time":"2018-05-21T21:36:10Z","timestamp":1526938570000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Sustained conduction of vasomotor responses in rat mesenteric arteries in a two\u2010compartment in\u00a0vitro set\u2010up"],"prefix":"10.1111","volume":"224","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1291-7153","authenticated-orcid":false,"given":"T.","family":"Palao","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering and Physics Academic Medical Center Amsterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"van Weert","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering and Physics Academic Medical Center Amsterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"de Leeuw","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering and Physics Academic Medical Center Amsterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"de Vos","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering and Physics Academic Medical Center Amsterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. N. T. P.","family":"Bakker","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering and Physics Academic Medical Center Amsterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"van Bavel","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering and Physics Academic Medical Center Amsterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,6,17]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.2010.02244.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1159\/000439112"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1978.sp012374"},{"key":"e_1_2_6_5_1","first-page":"H2022","article-title":"Electromechanical coupling and the conducted vasomotor response","volume":"269","author":"Xia J","year":"1995","journal-title":"Am J Physiol"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1998.274.1.H178"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.86.6.649"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000065918.90271.9A"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-010-0830-4"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3775368"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-201x.1999.00582.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2010.01148.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.2013.25"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00012.2014"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1998.275.2.H349"},{"key":"e_1_2_6_16_1","first-page":"H1364","article-title":"Acetylcholine induces conducted vasodilation by nitric oxide\u2010dependent and \u2010independent mechanisms","volume":"272","author":"Doyle MP","year":"1997","journal-title":"Am J Physiol"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1549-8719.2011.00127.x"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(99)00174-1"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1997.78.2.651"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiologyonline.1992.7.4.152"},{"key":"e_1_2_6_21_1","first-page":"F198","article-title":"Magnitude of TGF\u2010initiated nephron\u2010nephron interactions is increased in SHR","volume":"269","author":"Chen YM","year":"1995","journal-title":"Am J Physiol"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.26.2.163"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1113\/eph8702399"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1159\/000048989"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1159\/000077288"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1080\/10739680802229076"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1991.260.4.H1276"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1998.501bh.x"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0705870"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1995.269.6.H2031"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.12.6529"},{"key":"e_1_2_6_32_1","first-page":"H181","article-title":"Microvascular recruitment in hamster striated muscle: role for conducted vasodilation","volume":"261","author":"Segal SS","year":"1991","journal-title":"Am J Physiol"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.14.1.197"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.060343"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2004.070458"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1989.256.3.H838"},{"key":"e_1_2_6_37_1","first-page":"F372","article-title":"Internephron coupling by conducted vasomotor responses in normotensive and spontaneously hypertensive rats","volume":"272","author":"Wagner AJ","year":"1997","journal-title":"Am J Physiol"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-011-1049-8"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pharmtox-010617-052623"}],"container-title":["Acta Physiologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fapha.13099","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apha.13099","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,17]],"date-time":"2023-09-17T04:02:22Z","timestamp":1694923342000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/apha.13099"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,6,17]]},"references-count":38,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2018,11]]}},"alternative-id":["10.1111\/apha.13099"],"URL":"https:\/\/doi.org\/10.1111\/apha.13099","archive":["Portico"],"relation":{},"ISSN":["1748-1708","1748-1716"],"issn-type":[{"type":"print","value":"1748-1708"},{"type":"electronic","value":"1748-1716"}],"subject":[],"published":{"date-parts":[[2018,6,17]]},"assertion":[{"value":"2017-10-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-05-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-06-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13099"}}