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The consequences on renal and intestinal Ca<jats:sup>2+<\/jats:sup> and P<jats:sub>i<\/jats:sub> regulatory mechanisms are still unclear. We hypothesized that endotoxaemia alters the expression of important renal and intestinal Ca<jats:sup>2+<\/jats:sup> and P<jats:sub>i<\/jats:sub> transport proteins.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Male C57BL\/6 mice were treated with lipopolysaccharide (LPS; 3\u00a0mg\/kg; i.p.). The mRNA and protein levels of renal and intestinal Ca<jats:sup>2+<\/jats:sup> and P<jats:sub>i<\/jats:sub> transport proteins were measured by RT\u2010qPCR, immunohistochemistry and western blot analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Lipopolysaccharide\u2010induced hypocalcaemia and hyperphosphataemia was paralleled by a decrease in glomerular filtration rate and urinary excretion of Ca<jats:sup>2+<\/jats:sup> and P<jats:sub>i<\/jats:sub>. Endotoxaemia augmented plasma levels of PTH and affected the fibroblast growth factor 23 (FGF23)\u2010klotho\u2010vitamin D axis by increasing plasma levels of FGF23 and downregulation of renal klotho expression. Renal expression of CYP27b1 and plasma levels of 1,25\u2010dihydroxyvitamin D<jats:sub>3<\/jats:sub> were increased in response to LPS. Endotoxaemia augmented the renal expression of TRPV5, TRPV6 and PiT1, whereas the renal expression of calbindin\u2010D<jats:sub>28K<\/jats:sub>, NCX1, NaP<jats:sub>i<\/jats:sub>\u20102a and NaP<jats:sub>i<\/jats:sub>\u20102c were decreased. Incubation of primary distal tubule cells with LPS increased TRPV6 mRNA levels. Furthermore, LPS decreased the intestinal expression of TRPV6, calbindin\u2010D<jats:sub>9K<\/jats:sub> and of NaP<jats:sub>i<\/jats:sub>\u20102b.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our findings indicate that endotoxaemia is associated with hypocalcaemia and hyperphosphataemia and a disturbed FGF23\u2010klotho\u2010vitamin D signaling. Further, LPS\u2010induced acute kidney injury was accompanied by an increased or decreased expression of specific renal and intestinal Ca<jats:sup>2+<\/jats:sup> and P<jats:sub>i<\/jats:sub> transporters respectively. It seems unlikely that LPS\u2010induced hypocalcaemia is due to renal loss of Ca<jats:sup>2+<\/jats:sup>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/apha.13175","type":"journal-article","created":{"date-parts":[[2018,8,22]],"date-time":"2018-08-22T15:56:22Z","timestamp":1534953382000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Endotoxaemia differentially regulates the expression of renal Ca<sup>2+<\/sup> transport proteins in mice"],"prefix":"10.1111","volume":"225","author":[{"given":"Manuel","family":"Meurer","sequence":"first","affiliation":[{"name":"Institute of Experimental and Clinical Pharmacology and Toxicology Friedrich\u2010Alexander\u2010Universit\u00e4t Erlangen\u2010N\u00fcrnberg (FAU) Erlangen 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