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Number of c\u2010Fos+ cells, and CORT\/ACTH levels increased at 4\u2010hour post\u2010MPTP, followed by recovery at 12\u2010hour post\u2010MPTP. The <jats:italic>CRH<\/jats:italic> mRNA was elevated at 4\u2010hour post\u2010MPTP, and sustained for over 12\u00a0hours. At 2\u2010hour post\u2010MPTP, PVN\u2010injection of D2R agonist quinpirole corrected the increases in c\u2010Fos+ cells, CORT\/ACTH and <jats:italic>CRH<\/jats:italic> mRNA, but D1R agonist SKF38393 did not. PVN\u2010injection of D2R antagonist L\u2010sulpiride alone caused increases in c\u2010Fos+ cells, CORT\/ACTH and <jats:italic>CRH<\/jats:italic> mRNA. Similarly, PVN\u2010injection of CB1R agonist WIN552,12 prevented the increases in c\u2010Fos+ cells and CORT\/ACTH rather than <jats:italic>CRH<\/jats:italic> mRNA, which were blocked by CB1R antagonist AM251. Levels of PKA and CREB phosphorylation in PVN were increased at 4\u2010hour post\u2010MPTP, which were blocked by quinpirole, but not WIN552,12. PKA inhibitor H89 corrected the increase of <jats:italic>CRH<\/jats:italic> mRNA at 8\u2010hour post\u2010MPTP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The activation of dh\u2010DA neurons regulates negatively HPA axis through targeting D2Rs of CRH neurons to enhance endocannabinoid release and inhibit PKA\u2010CREB pathway.<\/jats:p><\/jats:sec>","DOI":"10.1111\/apha.13187","type":"journal-article","created":{"date-parts":[[2018,9,11]],"date-time":"2018-09-11T17:11:53Z","timestamp":1536685913000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Dorsal hypothalamic dopaminergic neurons play an inhibitory role in the hypothalamic\u2010pituitary\u2010adrenal axis via activation of D2R in mice"],"prefix":"10.1111","volume":"225","author":[{"given":"Tingting","family":"Di","sequence":"first","affiliation":[{"name":"State Key Lab of Reproductive Medicine Nanjing Medical University 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