{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T09:30:41Z","timestamp":1774344641108,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,4,26]],"date-time":"2019-04-26T00:00:00Z","timestamp":1556236800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Fonden","doi-asserted-by":"publisher","award":["11789"],"award-info":[{"award-number":["11789"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007405","name":"Hjerteforeningen","doi-asserted-by":"publisher","award":["R97-A5232"],"award-info":[{"award-number":["R97-A5232"]}],"id":[{"id":"10.13039\/100007405","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002261","name":"Russian Foundation for Basic Research","doi-asserted-by":"publisher","award":["16-44-730649"],"award-info":[{"award-number":["16-44-730649"]}],"id":[{"id":"10.13039\/501100002261","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Physiologica"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>This study aimed to assess intracellular Ca<jats:sup>2+<\/jats:sup> dynamics in nerve cells and Schwann cells in isolated rat resistance arteries and determine how these dynamics modify noradrenaline release from the nerves and consequent force development.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Ca<jats:sup>2+<\/jats:sup> in nerves was assessed with confocal imaging, noradrenaline release with amperometry and artery tone with wire myography. Ca<jats:sup>2+<\/jats:sup> in axons was assessed after loading with Oregon Green 488 BAPTA\u20101 dextran. In other experiments, arteries were incubated with Calcium Green\u20101\u2010AM which loads both axons and Schwann cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Schwann cells but not axons responded with a Ca<jats:sup>2+<\/jats:sup> increase to ATP. Electrical field stimulation of nerves caused a frequency\u2010dependent increase in varicose [Ca<jats:sup>2+<\/jats:sup>] ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>v<\/jats:sub>). \u03c9\u2010conotoxin\u2010GVIA (100\u00a0nmol\/L) reduced the [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>v<\/jats:sub> transient to 2 and 16\u00a0Hz by 60% and 27%, respectively; in contrast \u03c9\u2010conotoxin GVIA inhibited more than 80% of the noradrenaline release and force development at 2 and 16\u00a0Hz. The K<jats:sub>V<\/jats:sub> channel blocker, 4\u2010aminopyridine (10\u00a0\u00b5mol\/L), increased [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>v<\/jats:sub>, noradrenaline release and force development both in the absence and presence of \u03c9\u2010conotoxin\u2010GVIA. Yohimbine (1\u00a0\u00b5mol\/L) increased both [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>v<\/jats:sub> and noradrenaline release but reduced force development. Acetylcholine (10\u00a0\u00b5mol\/L) caused atropine\u2010sensitive inhibition of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>v<\/jats:sub>, noradrenaline release and force. In the presence of \u03c9\u2010conotoxin\u2010GVIA, acetylcholine caused a further inhibition of all parameters.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Modification of [Ca<jats:sup>2+<\/jats:sup>] in arterial sympathetic axons and Schwann cells was assessed separately. K<jats:sub>V<\/jats:sub>3.1 channels may be important regulators of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>v<\/jats:sub>, noradrenaline release and force development. Presynaptic adrenoceptor and muscarinic receptor activation modify transmitter release through modification of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>v<\/jats:sub>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/apha.13279","type":"journal-article","created":{"date-parts":[[2019,4,8]],"date-time":"2019-04-08T11:59:00Z","timestamp":1554724740000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["[Ca<sup>2+<\/sup>] changes in sympathetic varicosities and Schwann cells in rat mesenteric arteries\u2014Relation to noradrenaline release and contraction"],"prefix":"10.1111","volume":"226","author":[{"given":"Thomas","family":"Hansen","sequence":"first","affiliation":[{"name":"Department of Biomedicine University of Aarhus Aarhus C Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga S.","family":"Tarasova","sequence":"additional","affiliation":[{"name":"Faculty of Biology M.V. Lomonosov Moscow State University Moscow Russia"},{"name":"State Research Center of the Russian Federation \u2013 Institute for Biomedical Problems Moscow Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makhala M.","family":"Khammy","sequence":"additional","affiliation":[{"name":"Department of Biomedicine University of Aarhus Aarhus C Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Avelino","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Department of Biomedicine University of Aarhus Aarhus C Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James A.","family":"Kennard","sequence":"additional","affiliation":[{"name":"Institute of Clinical Sciences, College of Medical and Dental Sciences University of Birmingham Birmingham UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f8rgen","family":"Andresen","sequence":"additional","affiliation":[{"name":"Department of Biomedicine University of Aarhus Aarhus C Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Staehr","sequence":"additional","affiliation":[{"name":"Department of Biomedicine University of Aarhus Aarhus C Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keith L.","family":"Brain","sequence":"additional","affiliation":[{"name":"Institute of Clinical Sciences, College of Medical and Dental Sciences University of Birmingham Birmingham UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2350-7157","authenticated-orcid":false,"given":"Holger","family":"Nilsson","sequence":"additional","affiliation":[{"name":"Department of Physiology, Institute of Neuroscience and Physiology Sahlgrenska Academy at the University of Gothenburg Gothenburg Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2618-4015","authenticated-orcid":false,"given":"Christian","family":"Aalkj\u00e6r","sequence":"additional","affiliation":[{"name":"Department of Biomedicine University of Aarhus Aarhus C Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,4,26]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00187924"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1997.521bj.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(01)00280-9"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2001.0003k.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702984"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00772.2004"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.wnr.0000209001.09987.77"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-0173(99)00093-4"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702246"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0773.2003.920505.x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2012.08.002"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2005.10.006"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1999.tb11293.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-199107000-00002"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1472-8206.2002.00091.x"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1161\/hh0801.089603"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.41.1.19"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1988.sp017260"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.1985.tb07592.x"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.1984.tb07466.x"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.13347"}],"container-title":["Acta Physiologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apha.13279","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/apha.13279","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apha.13279","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T04:58:10Z","timestamp":1694321890000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/apha.13279"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4,26]]},"references-count":21,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2019,8]]}},"alternative-id":["10.1111\/apha.13279"],"URL":"https:\/\/doi.org\/10.1111\/apha.13279","archive":["Portico"],"relation":{},"ISSN":["1748-1708","1748-1716"],"issn-type":[{"value":"1748-1708","type":"print"},{"value":"1748-1716","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4,26]]},"assertion":[{"value":"2018-07-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13279"}}