{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T14:55:24Z","timestamp":1790434524189,"version":"4.1.0"},"reference-count":66,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,12,11]],"date-time":"2019-12-11T00:00:00Z","timestamp":1576022400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005363","name":"Universidad de Buenos Aires","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005363","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Physiologica"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>It is widely accepted that the exocytosis of synaptic and secretory vesicles is triggered by Ca<jats:sup>2+<\/jats:sup> entry through voltage\u2010dependent Ca<jats:sup>2+<\/jats:sup> channels. However, there is evidence of an alternative mode of exocytosis induced by membrane depolarization but lacking Ca<jats:sup>2+<\/jats:sup> current and intracellular Ca<jats:sup>2+<\/jats:sup> increase. In this work we investigated if such a mechanism contributes to secretory vesicle exocytosis in mouse chromaffin cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Exocytosis was evaluated by patch\u2010clamp membrane capacitance measurements, carbon fibre amperometry and TIRF. Cytosolic Ca<jats:sup>2+<\/jats:sup> was estimated using epifluorescence microscopy and fluo\u20108 (salt form).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Cells stimulated by brief depolatizations in absence of extracellular Ca<jats:sup>+2<\/jats:sup> show moderate but consistent exocytosis, even in presence of high cytosolic BAPTA concentration and pharmacological inhibition of Ca<jats:sup>+2<\/jats:sup> release from intracellular stores. This exocytosis is tightly dependent on membrane potential, is inhibited by neurotoxin Bont\u2010B (cleaves the v\u2010SNARE synaptobrevin), is very fast (saturates with time constant &lt;10\u00a0ms), it is followed by a fast endocytosis sensitive to the application of an anti\u2010dynamin monoclonal antibody, and recovers after depletion in &lt;5\u00a0s. Finally, this exocytosis was inhibited by: (i) \u03c9\u2010agatoxin IVA (blocks P\/Q\u2010type Ca<jats:sup>2+<\/jats:sup> channel gating), (ii) in cells from <jats:italic>knock\u2010out<\/jats:italic> P\/Q\u2010type Ca<jats:sup>2+<\/jats:sup> channel mice, and (iii) transfection of free synprint peptide (interferes in P\/Q channel\u2010exocytic proteins association).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We demonstrated that Ca<jats:sup>2+<\/jats:sup>\u2010independent and voltage\u2010dependent exocytosis is present in chromaffin cells. This process is tightly coupled to membrane depolarization, and is able to support secretion during action potentials at low basal rates. P\/Q\u2010type Ca<jats:sup>2+<\/jats:sup> channels can operate as voltage sensors of this process.<\/jats:p><\/jats:sec>","DOI":"10.1111\/apha.13417","type":"journal-article","created":{"date-parts":[[2019,11,26]],"date-time":"2019-11-26T16:35:19Z","timestamp":1574786119000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Ca<sup>2+<\/sup>\u2010independent and voltage\u2010dependent exocytosis in mouse chromaffin cells"],"prefix":"10.1111","volume":"228","author":[{"given":"Jos\u00e9","family":"Moya\u2010D\u00edaz","sequence":"first","affiliation":[{"name":"Instituto de Fisiolog\u00eda, Biolog\u00eda Molecular y Neurociencias Departamento de Fisiolog\u00eda y Biolog\u00eda Molecular y Celular Facultad de Ciencias Exactas y Naturales Universidad de Buenos Aires Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas Buenos Aires 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