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Therapeutic persistence and reduced primitive stem cell function following cell delivery remains a critical hurdle for the clinical translation of stem cells in current approaches.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Cultured ASCs were derived from subcutaneous white adipose tissue isolated from mice fed a normal diet (ND). Unilateral hindlimb ischaemia model was induced in high\u2010fat diet (HFD)\u2010fed mice by femoral artery interruption, after which photoactivated and non\u2010light\u2010treated ASCs were injected into the tail vein of mice. Laser Doppler imaging was conducted to measure the blood flow reperfusion. Capillary density was measured in the ischaemic gastrocnemius muscle. mRNA levels of angiogenic factors were determined by reverse\u2010transcription polymerase chain reaction. Flow cytometry was used to determine the characterization of ASCs and endothelial progenitor cell (EPC). Human ASCs secretomes were analysed by liquid chromatography tandem mass spectrometry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Our study demonstrated that photoactivated ND\u2010ASCs prolonged functional blood flow perfusion and increased ASCs\u2010derived EPC and neovascularization 38\u00a0days after ligation, when compared with saline\u2010treated controls. Profiling analysis in ischaemic muscles showed upregulation of genes associated with pro\u2010angiogenic factors after injection of photoactivated ND\u2010ASCs when compared with the non\u2010light\u2010treated ASCs or saline treated HFD mice. Mass spectrometry revealed that light\u2010treated ASCs conditioned medium retained a more complete pro\u2010angiogenic activity with significant upregulation of angiogenesis related proteins.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our data demonstrates that photoactivated ND\u2010ASCs improve blood flow recovery and their injection may prove to be a useful strategy for the prevention and treatment of diabetic peripheral arterial disease.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/apha.13475","type":"journal-article","created":{"date-parts":[[2020,4,19]],"date-time":"2020-04-19T15:38:55Z","timestamp":1587310735000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Prolonged therapeutic effects of photoactivated adipose\u2010derived stem cells following ischaemic 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