{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T20:20:36Z","timestamp":1790886036559,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,7,23]],"date-time":"2020-07-23T00:00:00Z","timestamp":1595462400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Physiologica"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Several Na<jats:sup>+<\/jats:sup>\u2010dependent phosphate cotransporters, namely NaPi\u2010IIb\/SLC34A2, Pit\u20101\/SLC20A1 and Pit\u20102\/SLC20A2, are expressed at the apical membrane of enterocytes but their contribution to active absorption of phosphate is unclear. The aim of this study was to compare their pattern of mRNA expression along the small and large intestine and to analyse the effect of intestinal depletion of Pit\u20102 on phosphate homeostasis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Intestinal epithelial Pit\u20102\u2010deficient mice were generated by crossing floxed Pit\u20102 with villin\u2010Cre mice. Mice were fed 2\u00a0weeks standard or low phosphate diets. Stool, urine, plasma and intestinal and renal tissue were collected. Concentration of electrolytes and hormones, expression of mRNAs and proteins and intestinal transport of tracers were analysed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Intestinal mRNA expression of NaPi\u2010IIb and Pit\u20101 is segment\u2010specific, whereas the abundance of Pit\u20102 mRNA is more homogeneous. In ileum, NaPi\u2010IIb mRNA expression is restricted to enterocytes, whereas Pit\u20102 mRNA is found in epithelial and non\u2010epithelial cells. Overall, their mRNA expression is not regulated by dietary phosphate. The absence of Pit\u20102 from intestinal epithelial cells does not affect systemic phosphate homeostasis under normal dietary conditions. However, in response to dietary phosphate restriction, Pit\u20102\u2010deficient mice showed exacerbated hypercalciuria and sustained elevation of 1,25(OH)<jats:sub>2<\/jats:sub> vitamin D<jats:sub>3<\/jats:sub>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In mice, the intestinal Na<jats:sup>+<\/jats:sup>\/phosphate cotransporters are not coexpressed in all segments. NaPi\u2010IIb but not Pit\u20102 mRNA is restricted to epithelial cells. Intestinal epithelial Pit\u20102 does not contribute significantly to absorption of phosphate under normal dietary conditions. However, it may play a more significant role upon dietary phosphate restriction.<\/jats:p><\/jats:sec>","DOI":"10.1111\/apha.13526","type":"journal-article","created":{"date-parts":[[2020,6,21]],"date-time":"2020-06-21T19:04:11Z","timestamp":1592766251000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Intestinal epithelial ablation of Pit\u20102\/Slc20a2 in mice leads to sustained elevation of vitamin D<sub>3<\/sub> upon dietary restriction of phosphate"],"prefix":"10.1111","volume":"230","author":[{"given":"Eva M.","family":"Pastor\u2010Arroyo","sequence":"first","affiliation":[{"name":"Institute of Physiology University of Z\u00fcrich Z\u00fcrich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Kn\u00f6pfel","sequence":"additional","affiliation":[{"name":"Institute of Physiology University of Z\u00fcrich Z\u00fcrich 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