{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:44:33Z","timestamp":1787013873664,"version":"build-2736575974"},"reference-count":49,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,12,20]],"date-time":"2020-12-20T00:00:00Z","timestamp":1608422400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006197","name":"Fonden til L\u00e6gevidenskabens Fremme","doi-asserted-by":"publisher","award":["19\u2010L\u20100367"],"award-info":[{"award-number":["19\u2010L\u20100367"]}],"id":[{"id":"10.13039\/501100006197","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006308","name":"Helga og Peter Kornings Fond","doi-asserted-by":"publisher","award":["DC472123\u2010004\u2010127"],"award-info":[{"award-number":["DC472123\u2010004\u2010127"]}],"id":[{"id":"10.13039\/501100006308","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004836","name":"Det Frie Forskningsr\u00e5d","doi-asserted-by":"publisher","award":["610\u201000131"],"award-info":[{"award-number":["610\u201000131"]}],"id":[{"id":"10.13039\/501100004836","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Physiologica"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>\n                      Cystic fibrosis patients have an increased risk of developing metabolic alkalosis presumably as a result of altered renal HCO\n                      <jats:sub>3<\/jats:sub>\n                      <jats:sup>\u2212<\/jats:sup>\n                      handling. In this study, we directly assess the kidneys\u2019 ability to compensate for a chronic base\u2010load in the absence of functional CFTR.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Comprehensive urine and blood acid\u2010base analyses were done in anaesthetized WT mice or mice lacking either CFTR or pendrin, with or without 7\u00a0days of oral NaHCO\n                      <jats:sub>3<\/jats:sub>\n                      loading. The in vivo experiments were complemented by a combination of immunoblotting and experiments with perfused isolated mouse cortical collecting ducts (CCD).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Base\u2010loaded WT mice maintained acid\u2010base homeostasis by elevating urinary pH and HCO\n                      <jats:sub>3<\/jats:sub>\n                      <jats:sup>\u2212<\/jats:sup>\n                      excretion and decreasing urinary net acid excretion. In contrast, pendrin KO mice and CFTR KO mice were unable to increase urinary pH and HCO\n                      <jats:sub>3<\/jats:sub>\n                      <jats:sup>\u2212<\/jats:sup>\n                      excretion and unable to decrease urinary net acid excretion sufficiently and thus developed metabolic alkalosis in response to the same base\u2010load. The expression of pendrin was increased in response to the base\u2010load in WT mice with a paralleled increased pendrin function in the perfused CCD. In CFTR KO mice, 7\u00a0days of base\u2010loading did not upregulate pendrin expression and apical Cl\n                      <jats:sup>\u2212<\/jats:sup>\n                      \/HCO\n                      <jats:sub>3<\/jats:sub>\n                      <jats:sup>\u2212<\/jats:sup>\n                      exchange function was strongly blunted in the CCD.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      CFTR KO mice develop metabolic alkalosis during a chronic base\u2010load because they are unable to sufficiently elevate renal HCO\n                      <jats:sub>3<\/jats:sub>\n                      <jats:sup>\u2212<\/jats:sup>\n                      excretion. This can be explained by markedly reduced pendrin function in the absence of CFTR.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/apha.13591","type":"journal-article","created":{"date-parts":[[2020,12,3]],"date-time":"2020-12-03T09:26:04Z","timestamp":1606987564000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Impaired renal HCO\n                    <sub>3<\/sub>\n                    <sup>\u2212<\/sup>\n                    secretion in CFTR deficient mice causes metabolic alkalosis during chronic base\u2010loading"],"prefix":"10.1111","volume":"231","author":[{"given":"Peder","family":"Berg","sequence":"first","affiliation":[{"name":"Department of Biomedicine, Physiology Faculty of Health Aarhus University Aarhus C Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel L.","family":"Svendsen","sequence":"additional","affiliation":[{"name":"Department of Biomedicine, Physiology Faculty of Health Aarhus University Aarhus C Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thi Thuy Linh","family":"Hoang","sequence":"additional","affiliation":[{"name":"Department of Biomedicine, Physiology Faculty of Health Aarhus University Aarhus C Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helle A.","family":"Praetorius","sequence":"additional","affiliation":[{"name":"Department of Biomedicine, Physiology Faculty of Health Aarhus University Aarhus C Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mads V.","family":"Sorensen","sequence":"additional","affiliation":[{"name":"Department of Biomedicine, Physiology Faculty of Health Aarhus University Aarhus C Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6719-7034","authenticated-orcid":false,"given":"Jens","family":"Leipziger","sequence":"additional","affiliation":[{"name":"Department of Biomedicine, Physiology Faculty of Health Aarhus University Aarhus C Denmark"},{"name":"Aarhus Institute of Advanced Studies Aarhus University Aarhus C Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,12,20]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(09)60327-5"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00467-013-2712-4"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874306401206010059"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.124.2.490"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000487691"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2020010053"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1990.246"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-physiol-021014-071854"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2018-0158OC"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.266734"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1159\/000335115"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.071516798"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00033.2010"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00474.2005"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.beem.2017.04.011"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1530\/EJE-11-0101"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1115"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12551-012-0068-9"},{"key":"e_1_2_9_20_1","first-page":"F237","article-title":"CFTR expression in cortical collecting duct cells","volume":"270","author":"Todd\u2010Turla KM","year":"1996","journal-title":"Am J Physiol"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.122.2.755-a"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1159\/000168839"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00254.2002"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2016080930"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00188.2001"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2006030269"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601387"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1159\/000335104"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2010.04.004"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1042\/CS20180060"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198808043190502"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1136\/adc.65.7.786"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2016030279"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.2215\/CJN.08000520"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1097\/MNH.0b013e3282e7d7d0"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2013.138"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2014.02.001"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.039818"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/apha.13405"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/10.2.153"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471234303.ch4"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-017-1997-8"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-9120(72)80014-6"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00582110"},{"key":"e_1_2_9_45_1","first-page":"F957","article-title":"Use of fluorescent dye BCECF to measure intracellular pH in cortical collecting tubule","volume":"256","author":"Weiner ID","year":"1989","journal-title":"Am J Physiol"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114423"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00578a012"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00010.2016"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00037.2002"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr1011476"}],"container-title":["Acta Physiologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apha.13591","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/apha.13591","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apha.13591","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T21:52:30Z","timestamp":1693605150000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/apha.13591"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12,20]]},"references-count":49,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2021,3]]}},"alternative-id":["10.1111\/apha.13591"],"URL":"https:\/\/doi.org\/10.1111\/apha.13591","archive":["Portico"],"relation":{},"ISSN":["1748-1708","1748-1716"],"issn-type":[{"value":"1748-1708","type":"print"},{"value":"1748-1716","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,12,20]]},"assertion":[{"value":"2020-04-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13591"}}