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BASP1 (brain acid\u2010soluble protein) is up\u2010regulated in podocyte\u2010specific protein phosphatase 2A knockout mice (Pod\u2010PP2A\u2010KO) that develop kidney dysfunction. Here, we explore the role of BASP1 for podocytes in DN.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>BASP1 was assessed in kidneys from DN patients and DN mouse models, podocyte specific BASP1 knockout mice (Pod\u2010BASP1\u2010KO mice) were generated and studied in vivo. Furthermore, podocyte injury and apoptosis were measured after BASP1 knockdown and overexpression in a mouse podocyte cell line (MPC5). Potential signalling pathways involved in podocyte apoptosis were detected.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>BASP1 expression was up\u2010regulated in DN patients compared to normal controls. BASP1 specific deletion in podocytes protected against podocyte injury by reducing the loss of expression of slit diaphragm molecules and foot process effacement in the DN model. BASP1 promoted actin cytoskeleton rearrangements and apoptosis in the MPC5 podocyte line. Molecules involved in the p53 pathway were down\u2010regulated in BASP1 knockdown podocytes treated with high glucose compared to controls. BASP1 promoted podocyte apoptosis and P53 pathway activation through co\u2010repression with Wilms' tumour 1 transcription factor (WT1).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>BASP1 activates the p53 pathway through modulation of WT1 to induce podocyte apoptosis in diabetic nephropathy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/apha.13634","type":"journal-article","created":{"date-parts":[[2021,2,22]],"date-time":"2021-02-22T08:11:15Z","timestamp":1613981475000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Podocyte apoptosis in diabetic nephropathy by BASP1 activation of the p53 pathway via WT1"],"prefix":"10.1111","volume":"232","author":[{"given":"Yingying","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Nephrology The Children Hospital of Zhejiang University School of Medicine National Clinical Research Center for Child Health National Children\u2019s Regional Medical Center Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengxian","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Nephrology The Children Hospital of Zhejiang University School of Medicine National Clinical Research Center for Child Health National Children\u2019s Regional Medical Center Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Nephrology The Children Hospital of Zhejiang University School of Medicine National Clinical Research Center for Child Health National Children\u2019s Regional Medical Center Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingxiao","family":"Tong","sequence":"additional","affiliation":[{"name":"Department of Nephrology The Children Hospital of Zhejiang University School of Medicine National Clinical Research Center for Child Health National Children\u2019s Regional Medical Center Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Jiang","sequence":"additional","affiliation":[{"name":"Kidney Disease Center The First Affiliated Hospital Zhejiang University School of Medicine Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiujuan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Nephrology The Children Hospital of Zhejiang University School of Medicine National Clinical Research Center for Child Health National Children\u2019s Regional Medical Center Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Limin","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Nephrology The Children Hospital of Zhejiang University School of Medicine National Clinical Research Center for Child Health National Children\u2019s Regional Medical Center Hangzhou 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