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We hypothesized that stellate ganglia (SG) neural inflammation contributes to ALI\u2010induced arrhythmia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We created an ALI rat model using a single tracheal instillation of bleomycin (2.5\u00a0mg\/kg), with saline as a sham control. We recorded ECGs by implanted radiotelemetry in male bleomycin and sham rats treated with and without oral minocycline (20\u00a0mg\/kg\/d), an anti\u2010inflammatory drug that inhibits microglia\/macrophage activation. The SG neuronal excitability was assessed by electrophysiology experiments.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>ECG data showed that bleomycin\u2010exposed rats exhibited significantly more spontaneous premature ventricular contractions (PVCs) from 1\u2010 to 3\u2010week post\u2010induction compared with sham rats, which was mitigated by chronic oral administration of minocycline. The bleomycin\u2010exposed rats displayed a robust increase in both the number of Iba1\u2010positive macrophages and protein expression of interferon regulatory factor 8 in the SG starting as early at 1\u2010week post\u2010exposure and lasted for at least 4\u00a0weeks, which was largely attenuated by minocycline. Heart rate variability analysis indicated autonomic imbalance during the first 2\u2010week post\u2010bleomycin, which was significantly attenuated by minocycline. Electrical stimulation of the decentralized SG triggered more PVCs in bleomycin\u2010exposed rats than sham and bleomycin\u00a0+\u00a0minocycline rats. Patch\u2010clamp data demonstrated enhanced SG neuronal excitability in the bleomycin\u2010exposed rats, which was attenuated by minocycline. Co\u2010culture of lipopolysaccharide (LPS)\u2010pretreated macrophages with normal SG neurons enhanced SG neuronal excitability.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Macrophage activation in the SG contributes to arrhythmogenesis in bleomycin\u2010induced ALI in male rats.<\/jats:p><\/jats:sec>","DOI":"10.1111\/apha.13657","type":"journal-article","created":{"date-parts":[[2021,4,5]],"date-time":"2021-04-05T07:50:37Z","timestamp":1617609037000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Macrophage activation in stellate ganglia contributes to lung injury\u2010induced arrhythmogenesis in male rats"],"prefix":"10.1111","volume":"232","author":[{"given":"Juan","family":"Hong","sequence":"first","affiliation":[{"name":"Department of Anesthesiology University of Nebraska Medical Center Omaha NE USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan 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