{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T16:07:43Z","timestamp":1773677263424,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,1,12]],"date-time":"2023-01-12T00:00:00Z","timestamp":1673481600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004742","name":"National Academy of Sciences of Ukraine","doi-asserted-by":"publisher","award":["0122U002126"],"award-info":[{"award-number":["0122U002126"]}],"id":[{"id":"10.13039\/501100004742","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007684","name":"Ministry of Education and Science of Ukraine","doi-asserted-by":"publisher","award":["0122U001535"],"award-info":[{"award-number":["0122U001535"]}],"id":[{"id":"10.13039\/501100007684","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Physiologica"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Gold nanoparticles are widely used for biomedical applications, but the precise molecular mechanism of their interaction with cellular structures is still unclear. Assuming that intracellular calcium fluctuations associated with surface plasmon\u2010induced calcium entry could modulate the activity of potassium channels, we studied the effect of 5\u00a0nm gold nanoparticles on calcium\u2010dependent potassium channels and associated calcium signaling in freshly isolated rat pulmonary artery smooth muscle cells and cultured hippocampal neurons.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Outward potassium currents were recorded using patch\u2010clamp techniques. Changes in intracellular calcium concentration were measured using the high affinity Ca<jats:sup>2+<\/jats:sup> fluorescent indicator fluo\u20103 and laser confocal microscope.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In pulmonary artery smooth muscle cells, plasmonic gold nanoparticles increased the amplitude of currents via large\u2010conductance Ca<jats:sup>2+<\/jats:sup>\u2010activated potassium channels, which was potentiated by green laser irradiation near plasmon resonance wavelength (532\u2009nm). Buffering of intracellular free calcium with ethylene glycol\u2010bis\u2010N,N,N\u2032,N\u2032\u2010tetraacetic acid (EGTA) abolished these effects. Furthermore, using confocal laser microscopy it was found that application of gold nanoparticles caused oscillations of intracellular calcium concentration that were decreasing in amplitude with time. In cultured hippocampal neurons gold nanoparticles inhibited the effect of EGTA slowing down the decline of the BK<jats:sub>Ca<\/jats:sub> current while partially restoring the amplitude of the slow after hyperpolarizing currents.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We conclude that fluctuations in intracellular calcium can modulate plasmonic gold nanoparticles\u2010induced gating of BK<jats:sub>Ca<\/jats:sub> channels in smooth muscle cells and neurons through an indirect mechanism, probably involving the interaction of plasmon resonance with calcium\u2010permeable ion channels, which leads to a change in intracellular calcium level.<\/jats:p><\/jats:sec>","DOI":"10.1111\/apha.13922","type":"journal-article","created":{"date-parts":[[2023,1,5]],"date-time":"2023-01-05T00:54:42Z","timestamp":1672880082000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Calcium\u2010dependent modulation of BK<sub>Ca<\/sub> channel activity induced by plasmonic gold nanoparticles in pulmonary artery smooth muscle cells and hippocampal neurons"],"prefix":"10.1111","volume":"237","author":[{"given":"Anatoly","family":"Soloviev","sequence":"first","affiliation":[{"name":"Department of Pharmacology of Cell Signaling Systems and Experimental Therapeutics Institute of Pharmacology and Toxicology, National Academy of Medical Science of Ukraine  Kyiv Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irina","family":"Ivanova","sequence":"additional","affiliation":[{"name":"Department of Pharmacology of Cell Signaling Systems and Experimental Therapeutics Institute of Pharmacology and Toxicology, National Academy of Medical Science of Ukraine  Kyiv Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0817-5783","authenticated-orcid":false,"given":"Vadym","family":"Sydorenko","sequence":"additional","affiliation":[{"name":"Department of Pharmacology of Cell Signaling Systems and Experimental Therapeutics Institute of Pharmacology and Toxicology, National Academy of Medical Science of Ukraine  Kyiv Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khrystyna","family":"Sukhanova","sequence":"additional","affiliation":[{"name":"McKelvey School of Engineering, Washington University in St. Louis  St. Louis Missouri USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariia","family":"Melnyk","sequence":"additional","affiliation":[{"name":"Department of Biophysics and Medical Informatics Educational and Scientific Centre \u201cInstitute of Biology and Medicine\u201d, Taras Shevchenko National University of Kyiv  Kyiv Ukraine"},{"name":"Department of Cellular Membranology A.A. Bogomoletz Institute of Physiology  Kyiv Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dariia","family":"Dryn","sequence":"additional","affiliation":[{"name":"Department of Cellular Membranology A.A. Bogomoletz Institute of Physiology  Kyiv Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Zholos","sequence":"additional","affiliation":[{"name":"Department of Biophysics and Medical Informatics Educational and Scientific Centre \u201cInstitute of Biology and Medicine\u201d, Taras Shevchenko National University of Kyiv  Kyiv Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,1,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2004.03.032"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2017.11.018"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.7b06600"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/1095148"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.irn.2016.05.003"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.14792"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms6847"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nantod.2016.12.014"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S10466"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vph.2015.05.016"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1088\/1361-648X\/aa60f3"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201505534"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2015.02.033"},{"key":"e_1_2_7_15_1","doi-asserted-by":"crossref","unstructured":"PavioloC HaycockJW YongJ YuA McArthurSL StoddartPR.Plasmonic properties of gold nanoparticles can promote neuronal activity. Proceedings volume 8579 Opt. Interact. with Tissue and Cells XXIV2013;8579:C1\u201014.","DOI":"10.1117\/12.2002291"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.25984"},{"issue":"4","key":"e_1_2_7_17_1","first-page":"203","article-title":"Calcium\u2010dependent regulation of ion channels","volume":"1","author":"Shah NV","year":"2006","journal-title":"Calcium Bind Proteins"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2010.06.004"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.2000.83.5.2554"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1986.55.6.1268"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1987.sp016653"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1998.80.5.2268"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1995.74.6.2225"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2004.02.021"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1908183117"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.20028605"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004240050801"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1132915"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nano.2019.03.018"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2017.11.013"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.8.000177"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep20619"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/nn1000222"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2010.02.117"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.56.4.1"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2010.07.013"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00583.2003"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2007.01.011"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2010.07303.x"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1998.707bs.x"}],"container-title":["Acta Physiologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apha.13922","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/apha.13922","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apha.13922","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,18]],"date-time":"2023-08-18T09:54:32Z","timestamp":1692352472000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/apha.13922"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,12]]},"references-count":39,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2023,3]]}},"alternative-id":["10.1111\/apha.13922"],"URL":"https:\/\/doi.org\/10.1111\/apha.13922","archive":["Portico"],"relation":{},"ISSN":["1748-1708","1748-1716"],"issn-type":[{"value":"1748-1708","type":"print"},{"value":"1748-1716","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,12]]},"assertion":[{"value":"2022-03-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-01-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-01-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13922"}}