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We hypothesized a similar function in the intracerebral arterioles.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Intracerebral arterioles were isolated from wild type C57BL\/6J mouse (9\u201312\u2009months old) brains and from human brain biopsies. The function (contractility and secondary dilatation) of the intracerebral arterioles was tested ex vivo by pressure myography using a perfusion set\u2010up. Reverse transcription polymerase chain reaction was used for detecting CSE expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CSE is expressed in human and mouse intracerebral arterioles. CSE inhibition with L\u2010propargylglycine (PAG) significantly dampened the K<jats:sup>+<\/jats:sup>\u2010induced vasoconstriction in intracerebral arterioles of both species (% of maximum contraction: in human control: 45.4\u2009\u00b1\u20092.7 versus PAG: 27\u2009\u00b1\u20095.2 and in mouse control: 50\u2009\u00b1\u20091.5 versus PAG: 33\u2009\u00b1\u20095.2) but did not affect the secondary dilatation. This effect of PAG was significantly reversed by the H<jats:sub>2<\/jats:sub>S donor sodium hydrosulfide (NaSH) in human (PAG\u2009+\u2009NaSH: 38.8\u2009\u00b1\u20097.2) and mouse (PAG\u2009+\u2009NaSH: 41.7\u2009\u00b1\u20093.1) arterioles, respectively. The endothelial NO synthase (eNOS) inhibitor, N\u03c9\u2010Nitro\u2010l\u2010arginine methyl ester (L\u2010NAME), and the inhibitor of soluble guanylate cyclase (sGC), 1H\u2010[1,2,4]oxadiazolo[4,3\u2010a]quinoxalin\u20101\u2010one (ODQ) reversed the effect of PAG on the K<jats:sup>+<\/jats:sup>\u2010induced vasoconstriction in the mouse arterioles and attenuated the K<jats:sup>+<\/jats:sup>\u2010induced secondary dilatation significantly.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>CSE contributes to the K<jats:sup>+<\/jats:sup>\u2010induced vasoconstriction via a mechanism involving H<jats:sub>2<\/jats:sub>S, eNOS, and sGC whereas the secondary dilatation is regulated by eNOS and sGC but not by CSE.<\/jats:p><\/jats:sec>","DOI":"10.1111\/apha.14021","type":"journal-article","created":{"date-parts":[[2023,8,9]],"date-time":"2023-08-09T12:33:40Z","timestamp":1691584420000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Inhibition of cystathionine\u2010gamma lyase dampens vasoconstriction in mouse and human intracerebral arterioles"],"prefix":"10.1111","volume":"239","author":[{"given":"Maria","family":"Peleli","sequence":"first","affiliation":[{"name":"Department of Medical Biochemistry and Microbiology, Science for Life Laboratory Uppsala University  Uppsala Sweden"},{"name":"Department of Cardiovascular and Renal Research, Institute of Molecular Medicine University of Southern Denmark  Odense Denmark"},{"name":"Laboratory of Pharmacology, Department of Pharmacy National and Kapodistrian University of Athens  Athens Greece"},{"name":"Clinical, Experimental Surgery and Translational Research Center Biomedical Research Foundation of the Academy of Athens  Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3250-4049","authenticated-orcid":false,"given":"Kristina S.","family":"Lyngso","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular and Renal Research, Institute of Molecular Medicine University of Southern Denmark  Odense Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frantz Rom","family":"Poulsen","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Odense University Hospital  Odense Denmark"},{"name":"Department of Clinical Research University of Southern Denmark and BRIDGE (Brain Research\u2010Interdisciplinary Guided Excellence)  Odense Denmark"},{"name":"OPEN \u2010 Odense Patient Data Explorative Network  Odense Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pernille B. 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