{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T05:08:06Z","timestamp":1790312886641,"version":"4.1.0"},"reference-count":54,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,12,9]],"date-time":"2023-12-09T00:00:00Z","timestamp":1702080000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Physiologica"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Bradyarrhythmias result from inhibition of automaticity, prolonged repolarization, or slow conduction in the heart. The ERG channels mediate the repolarizing current I<jats:sub>Kr<\/jats:sub> in the cardiac action potential, whereas T\u2010type calcium channels (TTCC) are involved in the sinoatrial pacemaker and atrioventricular conduction in mammals. Zebrafish have become a valuable research model for human cardiac electrophysiology and disease. Here, we investigate the contribution of ERG channels and TTCCs to the pacemaker and atrioventricular conduction in zebrafish larvae and determine the mechanisms causing atrioventricular block.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Zebrafish larvae expressing ratiometric fluorescent Ca<jats:sup>2+<\/jats:sup> biosensors in the heart were used to measure Ca<jats:sup>2+<\/jats:sup> levels and rhythm in beating hearts in\u00a0vivo, concurrently with contraction and hemodynamics. The atrioventricular delay (the time between the start of atrial and ventricular Ca<jats:sup>2+<\/jats:sup> transients) was used to measure impulse conduction velocity and distinguished between slow conduction and prolonged refractoriness as the cause of the conduction block.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>ERG blockers caused bradycardia and atrioventricular block by prolonging the refractory period in the atrioventricular canal and in working ventricular myocytes. In contrast, inhibition of TTCCs caused bradycardia and second\u2010degree block (Mobitz type I) by slowing atrioventricular conduction. TTCC block did not affect ventricular contractility, despite being highly expressed in cardiomyocytes. Concomitant measurement of Ca<jats:sup>2+<\/jats:sup> levels and ventricular size showed mechano\u2010mechanical coupling: increased preload resulted in a stronger heart contraction in\u00a0vivo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>ERG channels and TTCCs influence the heart rate and atrioventricular conduction in zebrafish larvae. The zebrafish lines expressing Ca<jats:sup>2+<\/jats:sup> biosensors in the heart allow us to investigate physiological feedback mechanisms and complex arrhythmias.<\/jats:p><\/jats:sec>","DOI":"10.1111\/apha.14075","type":"journal-article","created":{"date-parts":[[2023,12,10]],"date-time":"2023-12-10T05:33:27Z","timestamp":1702186407000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["ERG potassium channels and T\u2010type calcium channels contribute to the pacemaker and atrioventricular conduction in zebrafish larvae"],"prefix":"10.1111","volume":"240","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7421-6168","authenticated-orcid":false,"given":"Jussep","family":"Salgado\u2010Almario","sequence":"first","affiliation":[{"name":"Physiology and Cell Dynamics, Facultad de Medicina de Albacete Universidad de Castilla\u2010La Mancha  Albacete Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3425-4503","authenticated-orcid":false,"given":"Yillcer","family":"Molina","sequence":"additional","affiliation":[{"name":"Physiology and Cell Dynamics, Facultad de Medicina de Albacete Universidad de Castilla\u2010La Mancha  Albacete Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9464-918X","authenticated-orcid":false,"given":"Manuel","family":"Vicente","sequence":"additional","affiliation":[{"name":"Physiology and Cell Dynamics, Facultad de Medicina de Albacete Universidad de Castilla\u2010La Mancha  Albacete Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1337-308X","authenticated-orcid":false,"given":"Antonio","family":"Mart\u00ednez\u2010Sielva","sequence":"additional","affiliation":[{"name":"Physiology and Cell Dynamics, Facultad de Medicina de Albacete Universidad de Castilla\u2010La Mancha  Albacete Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ra\u00fal","family":"Rodr\u00edguez\u2010Garc\u00eda","sequence":"additional","affiliation":[{"name":"Physiology and Cell Dynamics, Facultad de Medicina de Albacete Universidad de Castilla\u2010La Mancha  Albacete Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8479-1908","authenticated-orcid":false,"given":"Pierre","family":"Vincent","sequence":"additional","affiliation":[{"name":"IGF, Univ. Montpellier, CNRS, INSERM  Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9430-4803","authenticated-orcid":false,"given":"Beatriz","family":"Domingo","sequence":"additional","affiliation":[{"name":"Physiology and Cell Dynamics, Facultad de Medicina de Albacete Universidad de Castilla\u2010La Mancha  Albacete Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9460-0642","authenticated-orcid":false,"given":"Juan","family":"Llopis","sequence":"additional","affiliation":[{"name":"Physiology and Cell Dynamics, Facultad de Medicina de Albacete Universidad de Castilla\u2010La Mancha  Albacete Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,12,9]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vascn.2013.10.002"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.15473"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/112_2016_5"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2014.03.011"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbiomolbio.2018.05.002"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/19336950.2015.1121335"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbiomolbio.2018.06.008"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.179226"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00960.2005"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep25073"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-013-1312-2"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00377.2010"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2010.04.157"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.110.003723"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2003.07.012"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.108.821082"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000225862.14314.49"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2009.08.021"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.4161\/chan.5.3.15266"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-76049-7"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1050-1738(00)00059-1"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2015.00019"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-021-03939-y"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-021-08016-z"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2009.08.034"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/membranes12060566"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.117.310230"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.64734"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2022.10.005"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2023.1086050"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1254\/jjp.69.303"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2012.01880.x"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0702724104"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.4070\/kcj.2017.0080"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.3904\/kjim.2019.038"},{"issue":"10","key":"e_1_2_10_37_1","first-page":"1294","article-title":"Cardioluminescence in transgenic zebrafish larvae: a calcium imaging tool to study drug effects and pathological modeling","volume":"9","author":"Vicente M","year":"2021","journal-title":"Biomedicine"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2773"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/tx800035b"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.26108"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000061912.88753.87"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2022.818122"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpr.2022.100046"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-014-1482-6"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21186610"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0125654"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0145163"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcdd9010013"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfz165"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2022.112695"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2019.00893"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.106.060244"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/0143-4160(90)90063-Z"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-622-1_14"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio732"}],"container-title":["Acta Physiologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apha.14075","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,31]],"date-time":"2024-01-31T12:46:26Z","timestamp":1706705186000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/apha.14075"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,9]]},"references-count":54,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,2]]}},"alternative-id":["10.1111\/apha.14075"],"URL":"https:\/\/doi.org\/10.1111\/apha.14075","archive":["Portico"],"relation":{},"ISSN":["1748-1708","1748-1716"],"issn-type":[{"value":"1748-1708","type":"print"},{"value":"1748-1716","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,12,9]]},"assertion":[{"value":"2023-08-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-11-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-09","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e14075"}}