{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T20:01:53Z","timestamp":1792008113196,"version":"4.4.0"},"reference-count":76,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,3,8]],"date-time":"2024-03-08T00:00:00Z","timestamp":1709856000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Physiologica"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>The influence on acute skeletal muscle transcriptomics of neuromuscular electrical stimulation (NMES), as compared to established exercises, is poorly understood. We aimed to investigate the effects on global mRNA\u2010expression in the quadriceps muscle early after a single NMES\u2010session, compared to the effects of voluntary knee extension exercise (EX), and to explore the discomfort level.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Global vastus lateralis muscle gene expression was assessed (RNA\u2010sequencing) in 30 healthy participants, before and 3\u2009h after a 30\u2010min session of NMES and\/or EX. The NMES\u2010treatment was applied using textile electrodes integrated in pants and set to 20% of each participant's pre\u2010tested MVC mean (\u00b1SD) 200 (\u00b180) Nm. Discomfort was assessed using Visual Analogue Scale (VAS, 0\u201310). The EX\u2010protocol was performed at 80% of 1\u2010repetition\u2010maximum.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>NMES at 20% of MVC resulted in VAS below 4 and induced 4448 differentially expressed genes (DEGs) with 80%\u2010overlap of the 2571 DEGs of EX. Genes well\u2010known to be up\u2010regulated following exercise, for example, PPARGC1A, ABRA, VEGFA, and GDNF, were also up\u2010regulated by NMES. Gene set enrichment analysis demonstrated many common pathways after EX and NMES. Also, some pathways were exclusive to either EX, for example, muscle tissue proliferation, or to NMES, for example, neurite outgrowth and connective tissue proliferation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>A 30\u2010min NMES\u2010session at 20% of MVC with NMES\u2010pants, which can be applied with an acceptable level of discomfort, induces over 4000 DEGs, of which 80%\u2010overlap with DEGs of EX. NMES can induce exercise\u2010like molecular effects, that potentially can lead to health and performance benefits in individuals who are unable to perform resistance exercise.<\/jats:p><\/jats:sec>","DOI":"10.1111\/apha.14129","type":"journal-article","created":{"date-parts":[[2024,3,9]],"date-time":"2024-03-09T07:24:27Z","timestamp":1709969067000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["The effect of neuromuscular electrical stimulation on the human skeletal muscle transcriptome"],"prefix":"10.1111","volume":"240","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0095-064X","authenticated-orcid":false,"given":"Johanna","family":"Flodin","sequence":"first","affiliation":[{"name":"Integrative Orthopedic Laboratory, Department of Molecular Medicine and Surgery Karolinska Institutet  Stockholm Sweden"},{"name":"Department of Trauma, Acute Surgery and Orthopedics Karolinska University Hospital  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0151-2780","authenticated-orcid":false,"given":"Stefan M.","family":"Reitzner","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pharmacology Karolinska Institutet  Stockholm Sweden"},{"name":"Department of Women's and Children's Health Karolinska Institutet  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric B.","family":"Emanuelsson","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pharmacology Karolinska Institutet  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carl Johan","family":"Sundberg","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pharmacology Karolinska Institutet  Stockholm Sweden"},{"name":"Department of Learning, Informatics, Management and Ethics Karolinska Institutet  Stockholm Sweden"},{"name":"Department of Laboratory Medicine, Division of Clinical Physiology Karolinska Institutet  Huddinge Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Ackermann","sequence":"additional","affiliation":[{"name":"Integrative Orthopedic Laboratory, Department of Molecular Medicine and Surgery Karolinska Institutet  Stockholm Sweden"},{"name":"Department of Trauma, Acute Surgery and Orthopedics Karolinska University Hospital  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,3,8]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2011.01.006"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2016.05.007"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/glu006"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2015.05.011"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/ageing\/afl084"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3310\/hta23630"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2017.02.070"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1109\/EMBC.2013.6611178"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/ptj\/74.10.901"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2014.08.004"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/17461391.2016.1151944"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/00002060-200103000-00011"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2014.00189"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.2165\/00007256-200535030-00002"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjpt.2018.03.006"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0069391"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1249\/MSS.0000000000002191"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1042\/CS20140447"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/SLA.0b013e3181a38e71"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.2147\/COPD.S189896"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1097\/PHM.0000000000001541"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00421-010-1502-y"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/bja\/aew466"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00421-022-04949-9"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.medengphy.2010.03.004"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1177\/1941738115618174"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1177\/11795441211028746"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apmr.2017.10.028"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/s150717241"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201600299"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10439-023-03349-0"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13102-022-00403-7"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jelekin.2023.102771"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3959(83)90126-4"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365517509095787"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00053.2021"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp616"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(88)92417-8"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.1800830320"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.129.6.1540"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardfail.2008.11.005"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/apha.12200"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygeno.2014.12.002"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10529-015-1963-5"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0074098"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2011.205468"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2012.00469"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M202216200"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.13624"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2009.168674"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2010.188011"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature00936.1"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80611-X"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.20.5.1868-1876.2000"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0115791"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7973664"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11515-012-1201-7"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1996.81.1.355"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00071.2004"},{"key":"e_1_2_11_61_1","doi-asserted-by":"publisher","DOI":"10.1089\/wound.2013.0427"},{"key":"e_1_2_11_62_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M306271200"},{"key":"e_1_2_11_63_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.302117"},{"key":"e_1_2_11_64_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA119.010060"},{"key":"e_1_2_11_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0736-0266(01)00159-0"},{"key":"e_1_2_11_66_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-33943-6_4"},{"key":"e_1_2_11_67_1","doi-asserted-by":"publisher","DOI":"10.2741\/4280"},{"key":"e_1_2_11_68_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00421-011-2128-4"},{"issue":"2","key":"e_1_2_11_69_1","first-page":"201","article-title":"Neuromuscular Electrical Stimulation for Skeletal Muscle Function","volume":"85","author":"Doucet BM","year":"2012","journal-title":"Yale J Biol Med"},{"key":"e_1_2_11_70_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph18052463"},{"key":"e_1_2_11_71_1","doi-asserted-by":"publisher","DOI":"10.1080\/17461391.2022.2104656"},{"key":"e_1_2_11_72_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00541.2003"},{"key":"e_1_2_11_73_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00559.2005"},{"key":"e_1_2_11_74_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2017.00297"},{"key":"e_1_2_11_75_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jelekin.2017.11.013"},{"key":"e_1_2_11_76_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0263662"},{"issue":"4","key":"e_1_2_11_77_1","first-page":"177","article-title":"Chronic neural adaptations to unilateral exercise: mechanisms of cross education","volume":"28","author":"Zhou S","year":"2000","journal-title":"Exerc Sport Sci Rev"}],"container-title":["Acta Physiologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apha.14129","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,4]],"date-time":"2024-05-04T06:52:48Z","timestamp":1714805568000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/apha.14129"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,8]]},"references-count":76,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2024,5]]}},"alternative-id":["10.1111\/apha.14129"],"URL":"https:\/\/doi.org\/10.1111\/apha.14129","archive":["Portico"],"relation":{},"ISSN":["1748-1708","1748-1716"],"issn-type":[{"value":"1748-1708","type":"print"},{"value":"1748-1716","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3,8]]},"assertion":[{"value":"2023-12-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e14129"}}