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Due to the broad spectrum of metabolites transported by SLC5A8 and SLC5A12, we aim to determine if they are functionally expressed and participate in the absorption of Na<jats:sup>+<\/jats:sup>, short\u2010chain fatty acids, and monocarboxylates in mouse and human airway epithelium.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Tracheas isolated from male or female mice and human bronchial epithelial cells (HBECs) were used for electrophysiological studies in the Ussing chamber and to detect members of the SLC16 family by RT\u2010PCR and bulk RNAseq. Additionally, cell lines expressing the human and murine SLC5A8 transporter were employed for uptake studies using a fluorescent lactate probe.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We showed for the first time that human and murine airway epithelium express a functional SLC5A8 transporter, facilitating the absorption of glucose metabolites and SCFAs. The Na<jats:sup>+<\/jats:sup>\u2010coupled monocarboxylate transport was not additive with ENaC\u2010mediated Na<jats:sup>+<\/jats:sup> absorption in mouse trachea. We observed that valproate acts as an inhibitor of the murine but not of the human SLC5A8 transporter.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our results demonstrate that several metabolites derived from bacterial and cellular metabolism can be transported from the airway lumen into the epithelial cells, participating in a homeostatic relation of the tissue with its environment.<\/jats:p><\/jats:sec>","DOI":"10.1111\/apha.70051","type":"journal-article","created":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T11:45:16Z","timestamp":1746531916000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Sodium\u2010Coupled Monocarboxylate Absorption in the Airway Epithelium Is Facilitated by the <scp>SLC5A8<\/scp> Co\u2010Transporter"],"prefix":"10.1111","volume":"241","author":[{"given":"Anita","family":"Guequen","sequence":"first","affiliation":[{"name":"Centro de Estudios Cient\u00edficos (CECs)  Valdivia Chile"},{"name":"Universidad Austral de Chile  Valdivia Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00e1rbara","family":"Tapia\u2010Balladares","sequence":"additional","affiliation":[{"name":"Centro de Estudios Cient\u00edficos (CECs)  Valdivia Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T\u00e1bata","family":"Apablaza","sequence":"additional","affiliation":[{"name":"Centro de Estudios Cient\u00edficos (CECs)  Valdivia Chile"},{"name":"Estudiante Programa de Doctorado en Enfermedades Cr\u00f3nicas, Facultad de Medicina Universidad San Sebasti\u00e1n  Valdivia Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Guidone","sequence":"additional","affiliation":[{"name":"Telethon Institute of Genetics and Medicine (TIGEM)  Pozzuoli Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-2506-3140","authenticated-orcid":false,"given":"N\u00e1tali","family":"C\u00e1rcamo\u2010Lemus","sequence":"additional","affiliation":[{"name":"Centro de Estudios Cient\u00edficos (CECs)  Valdivia Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Villanueva","sequence":"additional","affiliation":[{"name":"Centro de Estudios Cient\u00edficos (CECs)  Valdivia Chile"},{"name":"Facultad de Medicina Universidad San Sebasti\u00e1n  Valdivia Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6514-3980","authenticated-orcid":false,"given":"Pamela Y.","family":"Sandoval","sequence":"additional","affiliation":[{"name":"Centro de Estudios Cient\u00edficos (CECs)  Valdivia Chile"},{"name":"Facultad de Medicina Universidad San Sebasti\u00e1n  Valdivia Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis J. 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