{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T05:19:40Z","timestamp":1790140780948,"version":"4.0.1"},"reference-count":37,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,6,13]],"date-time":"2025-06-13T00:00:00Z","timestamp":1749772800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Physiologica"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Taste cells expressing GPR120 and connected chorda tympani nerve (CT) fibers are necessary for obtaining specific information on long\u2010chain fatty acids (FA) in mice. However, the functions of GPR40, GPR120, and CD36 in the posterior part of the tongue remain unclear. Therefore, the present study has examined the neuron types coding for FA information in the glossopharyngeal (GL) nerve and their functions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We performed single\u2010fiber recordings in the GL nerve from GPR120\u2010knockout (KO) and wild\u2010type (WT) mice and behavioral preference tests based on five\u2010minute intake using WT mice after the CT (and greater petrosal) nerves were sectioned bilaterally.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Single fibers were classified into FA\u2010(F), S\u2010, M\u2010, electrolyte\u2010(E), Q\u2010type, and N\u2010best groups, based on their maximal responses to oleic acid (OA), sucrose, monopotassium glutamate, HCl, quinine hydrochloride, and NaCl. Among the GL fibers, 3.8% of GPR120\u2010KO and 11.8% of WT mice were F\u2010type. Furthermore, 81.8% or more of the S\u2010 and M\u2010type fibers showed responses to FAs in both mouse genotypes. Residual responses to FAs were substantially suppressed by GPR40 and CD36 antagonists in GPR120\u2010KO mice. Preference scores for OA decreased significantly with the addition of CD36 or GPR40 antagonists. Additionally, the preference scores for monopotassium glutamate and sucrose decreased when the mice were conditioned to avoid OA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results suggest that GPR120 contributes only to the existence of F\u2010type fibers and that CD36 and GPR40 mediate the palatable umami or sweet taste of FAs via the activation of S\u2010 and M\u2010type fibers in the GL nerve.<\/jats:p><\/jats:sec>","DOI":"10.1111\/apha.70071","type":"journal-article","created":{"date-parts":[[2025,6,13]],"date-time":"2025-06-13T10:45:05Z","timestamp":1749811505000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Fatty Acid Taste Quality Information via <scp>GPR40<\/scp> and <scp>CD36<\/scp> in the Posterior Tongue of Mice"],"prefix":"10.1111","volume":"241","author":[{"given":"Yumiko","family":"Nagai","sequence":"first","affiliation":[{"name":"Tokyo Dental Junior College  Chiyoda\u2010ku Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenichi","family":"Tokita","sequence":"additional","affiliation":[{"name":"Institute of Natural Sciences Senshu University  Chiyoda\u2010ku Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1911-8792","authenticated-orcid":false,"given":"Keiko","family":"Yasumatsu","sequence":"additional","affiliation":[{"name":"Tokyo Dental Junior College  Chiyoda\u2010ku Tokyo Japan"},{"name":"Monell Chemical Senses Center  Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/apha.13529"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2011.211920"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(97)01055-7"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI25299"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0496-10.2010"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/apha.13215"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2530-20.2021"},{"key":"e_1_2_9_9_1","first-page":"83","volume-title":"Fat Detection: Taste, Texture, and Post Ingestive Effects","author":"Gilbertson T. A.","year":"2010"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0024014"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M025874"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.2220\/biomedres.28.49"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2014.01.006"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.acthis.2010.08.006"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/chemse\/bjr069"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00582.2012"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00211.2007"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.07-8415com"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2013.06.043"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00440.2013"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M021873"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu7115455"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/ijo.2015.20"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.9.5347"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2014.284703"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M707478200"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-physiol-032122-030352"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1100495108"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.2011.02308.x"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12684"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.R082933"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2013.06.017"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI99315"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006816702425"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2018.11.001"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-41637-4"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.23-10-04362.2003"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(82)90100-7"}],"container-title":["Acta Physiologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apha.70071","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,8]],"date-time":"2025-07-08T05:08:42Z","timestamp":1751951322000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/apha.70071"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,13]]},"references-count":37,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["10.1111\/apha.70071"],"URL":"https:\/\/doi.org\/10.1111\/apha.70071","archive":["Portico"],"relation":{},"ISSN":["1748-1708","1748-1716"],"issn-type":[{"value":"1748-1708","type":"print"},{"value":"1748-1716","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,13]]},"assertion":[{"value":"2025-01-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70071"}}