{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T19:10:43Z","timestamp":1788808243931,"version":"build-2803163510"},"reference-count":66,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T00:00:00Z","timestamp":1761868800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","award":["PID2022\u2010141928NB\u2010I00"],"award-info":[{"award-number":["PID2022\u2010141928NB\u2010I00"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["2022\u2010GRIN\u201034301"],"award-info":[{"award-number":["2022\u2010GRIN\u201034301"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007480","name":"Universidad de Castilla-La Mancha","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007480","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Physiologica"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>Heart failure is a clinical syndrome where the heart's structural or functional impairment leads to inadequate blood flow to meet the body's metabolic demands. Mitochondrial dysfunction is increasingly recognized as a central contributor underlying the contractile impairment observed in the failing heart. This study aimed to explore the interplay between calcium dynamics, cardiac mechanical performance, and mitochondrial ATP production during the progression of heart failure in zebrafish larvae exposed to chronic isoproterenol stimulation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Heart failure was induced by treating zebrafish larvae with 100\u2009\u03bcM isoproterenol from 3 to 14\u2009days postfertilization (dpf). Cardiac calcium transients, contractility, and mitochondrial ATP levels were assessed in\u00a0vivo using transgenic lines expressing specific fluorescent biosensors. Additionally, transcriptomic analysis by RNA sequencing was performed on hearts collected at 14\u2009dpf following prolonged isoproterenol exposure.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      After 4\u2009days of isoproterenol treatment (7\u2009dpf), larvae exhibited ventricular dilation, reduced calcium levels, and diminished contractile force (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001), although cardiac output remained intact. In contrast, extended treatment (11\u2009days; 14\u2009dpf) led to decompensated heart failure, characterized by a significant decline in cardiac output (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001). Mitochondrial ATP levels were preserved at 7\u2009dpf but dropped markedly at 14\u2009dpf (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001). Transcriptomic profiling at this later stage revealed downregulation of key functions (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05) involved in mitochondrial energy metabolism and energy transfer.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>In this model, heart dysfunction was initially evidenced by cardiac dilation. At 4\u2009days of isoproterenol treatment, calcium levels and contractility decreased. Subsequently, decompensation coincided with a collapse in mitochondrial ATP production.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/apha.70128","type":"journal-article","created":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T11:51:16Z","timestamp":1761911476000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Mitochondrial Dysfunction Contributes to Decompensation in a Zebrafish Model of Isoproterenol\u2010Induced Heart Failure"],"prefix":"10.1111","volume":"241","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9464-918X","authenticated-orcid":false,"given":"Manuel","family":"Vicente","sequence":"first","affiliation":[{"name":"Physiology and Cell Dynamics, Instituto de Biomedicina de la Universidad de Castilla\u2010La Mancha and Facultad de Medicina de Albacete Universidad de Castilla\u2010La Mancha  Albacete 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