{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T02:56:45Z","timestamp":1777949805683,"version":"3.51.4"},"reference-count":76,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T00:00:00Z","timestamp":1774742400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T00:00:00Z","timestamp":1774742400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["#2019\/12236\u20105"],"award-info":[{"award-number":["#2019\/12236\u20105"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["#2023\/01903\u20106"],"award-info":[{"award-number":["#2023\/01903\u20106"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["#2021\/05588\u20102"],"award-info":[{"award-number":["#2021\/05588\u20102"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["#403549\/2021\u20103"],"award-info":[{"award-number":["#403549\/2021\u20103"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["#420265\/2018\u20100"],"award-info":[{"award-number":["#420265\/2018\u20100"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["88887.827297\/2023\u201000"],"award-info":[{"award-number":["88887.827297\/2023\u201000"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Physiologica"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>The liver exhibits a remarkable regenerative capacity, enabling this organ to maintain homeostasis even after significant injury. However, hepatic regeneration requires sufficient energy to sustain cellular hypertrophy and proliferation, thus ensuring efficient tissue repair. Therefore, dietary modulation of pathways regulating mitochondrial quality may enhance liver regeneration. This study aimed to investigate the effects of preoperative beta\u2010hydroxy\u2010beta\u2010methylbutyrate (HMB) supplementation on mitochondrial quality control pathways and its impact on the liver regeneration process in mice undergoing partial hepatectomy (PHx).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Male C57BL\/6J mice were supplemented with 600\u2009mg\/kg HMB via\n                      <jats:italic>gavage<\/jats:italic>\n                      for 10\u2009days. On the 10th day, supplementation was discontinued, and the mice underwent a \u2154 liver resection. The subsequent 7\u2009days have constituted the liver regeneration period. For a second injury induction, at the end of the 7th day of regeneration, acetaminophen (APAP) overdose was administered via\n                      <jats:italic>gavage<\/jats:italic>\n                      . We then analyzed several markers of mitochondrial quality and liver function.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Our results indicate that preoperative HMB supplementation modulates cell cycle progression, preventing excessive hepatic mass accumulation. Additionally, HMB regulates mitochondrial dynamics by decreasing Parkin, Mfn2, and DRP1 protein levels while increasing the mitochondrial markers VDAC2 and Tom20. Following a second injury from an APAP overdose, the HMB\u2010supplemented group demonstrated increased mtDNA content and enhanced mitochondrial capacity, both critical for effective tissue recovery.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our findings suggest that preoperative HMB supplementation preserves hepatic mitochondrial capacity after PHx.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/apha.70204","type":"journal-article","created":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T03:27:13Z","timestamp":1774841233000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Preoperative Beta\u2010Hydroxy\u2010Beta\u2010Methyl\u2010Butyrate Supplementation Reduces Mitochondrial Dynamics Proteins and Preserves Hepatic Mitochondrial Function After Partial Hepatectomy in Mice"],"prefix":"10.1111","volume":"242","author":[{"given":"A. L.","family":"Vieira\u2010da\u2010Silva","sequence":"first","affiliation":[{"name":"Laboratory of Cell and Tissue Biology (LaBCeT), School of Applied Sciences University of Campinas  Limeira Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. V.","family":"Esteca","sequence":"additional","affiliation":[{"name":"Laboratory of Cell and Tissue Biology (LaBCeT), School of Applied Sciences University of Campinas  Limeira Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. A.","family":"Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Metabolic Disorders (LabDiMe), School of Applied Sciences University of Campinas  Limeira Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I. A.","family":"Divino","sequence":"additional","affiliation":[{"name":"Laboratory of Cell and Tissue Biology (LaBCeT), School of Applied Sciences University of Campinas  Limeira Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. S.","family":"Carneiro","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology of Exercise (LaBMEx), School of Applied Sciences University of Campinas  Limeira Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. R.","family":"Ropelle","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology of Exercise (LaBMEx), School of Applied Sciences University of Campinas  Limeira Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. S.","family":"Torsoni","sequence":"additional","affiliation":[{"name":"Laboratory of Metabolic Disorders (LabDiMe), School of Applied Sciences University of Campinas  Limeira Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5185-0185","authenticated-orcid":false,"given":"I. L.","family":"Baptista","sequence":"additional","affiliation":[{"name":"Laboratory of Cell and Tissue Biology (LaBCeT), School of Applied Sciences University of Campinas  Limeira Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,29]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-physiol-032822-094134"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trsl.2014.01.005"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00535-012-0569-0"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v29.i32.4815"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21218414"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1747\u20101028\u20108\u20108"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/4256352"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.4254\/wjh.v13.i3.270"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41575-020-0342-4"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hpb.2017.08.005"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00220.2022"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.10978"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2021.07.021"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.22979"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/hc9.0000000000000267"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1800025"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0899-9007(99)00158-6"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2008.11.021"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-805413-0.00023-5"},{"key":"e_1_2_10_21_1","first-page":"455","volume-title":"Nutrition and Enhanced Sports Performance: Muscle Building, Endurance, and Strength","author":"Pinheiro C. H. J.","year":"2014"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0117520"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12208"},{"issue":"1","key":"e_1_2_10_24_1","first-page":"37","article-title":"Beta\u2010Hydroxy\u2010Beta\u2010Methylbutyrate and Sarcopenia: From Biological Plausibility to Clinical Evidence","volume":"22","author":"Landi F.","year":"2019","journal-title":"Current Opinion in Clinical Nutrition and Metabolic Care"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/5546843"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.33549\/physiolres.933861"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0076752"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2014.122"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9FO00769E"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu15224706"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/nqy373"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu17071237"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.13394"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archger.2015.06.020"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10522\u2010025\u201010262\u20107"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.23059"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2019.00790"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2005.07.031"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI46039"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2019.01.011"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(200003)182:3<311::AID-JCP1>3.0.CO;2-9"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.20879"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0039\u20106060(98)70075\u20103"},{"key":"e_1_2_10_44_1","doi-asserted-by":"crossref","unstructured":"D. S.Williams \u201cHepatic Adenoma \u201d2020 https:\/\/jim.kglmeridian.com.","DOI":"10.17849\/insm-48-2-1-1.1"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2010.10.030"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-022-01107-7"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.22694"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2014-308410"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.3390\/life13061273"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.12669\/pjms.311.5864"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1080\/15384101.2016.1216924"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0017715"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1159\/000490030"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11745-016-4193-2"},{"key":"e_1_2_10_55_1","unstructured":"W.Li T.Cao C.Luo et\u00a0al. \u201cCrosstalk Between ER Stress NLRP3 Inflammasome and Inflammation \u201dhttps:\/\/doi.org\/10.1007\/s00253\u2010020\u201010614\u2010y\/Published."},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00195.2021"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2020.06.015"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.008227"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.3390\/livers1030010"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mito.2010.05.012"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010020\u201065438\u20107"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01605"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.08.041"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41418-020-00641-7"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.588616"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2009.89"},{"key":"e_1_2_10_67_1","unstructured":"D. C.Chan \u201cMitochondrial Dynamics and Its Involvement in Disease \u201d2019 https:\/\/doi.org\/10.1146\/annurev\u2010pathmechdis."},{"key":"e_1_2_10_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2007.03.091"},{"key":"e_1_2_10_69_1","doi-asserted-by":"crossref","unstructured":"R. A.Stancliffe \u201cRole of Beta\u2010Hydroxy\u2010Beta\u2010Methylbutyrate (HMB) in Leucine Role of Beta\u2010Hydroxy\u2010Beta\u2010Methylbutyrate (HMB) in Leucine Stimulation of Mitochondrial Biogenesis and Fatty Acid Oxidation Stimulation of Mitochondrial Biogenesis and Fatty Acid Oxidation \u201d2012 https:\/\/trace.tennessee.edu\/utk_gradthes\/1398.","DOI":"10.1096\/fasebj.26.1_supplement.251.6"},{"key":"e_1_2_10_70_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu15112550"},{"key":"e_1_2_10_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2024.114794"},{"key":"e_1_2_10_72_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2018.12.006"},{"key":"e_1_2_10_73_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0039-1679919"},{"key":"e_1_2_10_74_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep18017"},{"key":"e_1_2_10_75_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2017.08.020"},{"key":"e_1_2_10_76_1","volume-title":"Nutrient Metabolism","author":"Martin K.","year":"2015"},{"key":"e_1_2_10_77_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2021.10730"}],"container-title":["Acta Physiologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apha.70204","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/apha.70204","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/apha.70204","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T04:36:04Z","timestamp":1777869364000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/apha.70204"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,29]]},"references-count":76,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["10.1111\/apha.70204"],"URL":"https:\/\/doi.org\/10.1111\/apha.70204","archive":["Portico"],"relation":{},"ISSN":["1748-1708","1748-1716"],"issn-type":[{"value":"1748-1708","type":"print"},{"value":"1748-1716","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,29]]},"assertion":[{"value":"2025-07-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70204"}}