{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T02:56:40Z","timestamp":1777949800965,"version":"3.51.4"},"reference-count":45,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T00:00:00Z","timestamp":1775174400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T00:00:00Z","timestamp":1775174400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["207441"],"award-info":[{"award-number":["207441"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Physiologica"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>Renal sodium reabsorption occurs through both transcellular and paracellular pathways. Tight junction proteins play a key role in mediating paracellular transport. The collecting duct is critical for the fine tuning of sodium balance and is highly responsive to changes in dietary salt intake. This study aimed to determine whether a low\u2010sodium diet modulates paracellular sodium permeability by regulating the expression or localization of claudin\u20103, a major tight junction protein in the collecting duct.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Wild\u2010type and claudin\u20103 knockout male mice were fed low (0.01%) or normal (0.18%) sodium diets for 7\u2009days, with or without treatment with spironolactone, a mineralocorticoid receptor antagonist. The expression of tight junction proteins was analyzed by immunoblotting and immunofluorescence. Functional effects of claudin\u20103 on ion permeability were evaluated in cultured mouse collecting duct principal cells using chamber recordings after claudin\u20103 overexpression or gene silencing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Low\u2010sodium diet increases claudin\u20103 expression in mouse kidneys. In cultured cells, aldosterone enhanced claudin\u20103 abundance and its plasma membrane localization. Claudin\u20103 overexpression reduced, while its silencing increased paracellular permeability to sodium and chloride. Claudin\u20103 knockout mice on a low\u2010sodium diet compensated by upregulating epithelial sodium channel subunits, claudin\u20104, claudin\u20108, and claudin\u201010. This adaptive response persisted under mineralocorticoid receptor blockade.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our findings demonstrate that aldosterone strengthens the paracellular sodium barrier in the collecting duct by inducing claudin\u20103. In the absence of claudin\u20103, compensatory regulation of other claudins and sodium transporters preserves sodium homeostasis under low\u2010salt conditions, thus revealing adaptive mechanisms in renal sodium handling.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/apha.70216","type":"journal-article","created":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T10:06:12Z","timestamp":1775210772000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Low\u2010Salt Diet Induces Claudin\u20103 Expression and Drives Adaptive Changes in Collecting Duct of Claudin\u20103\u2010Deficient Mice"],"prefix":"10.1111","volume":"242","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3194-777X","authenticated-orcid":false,"given":"Ali","family":"Sassi","sequence":"first","affiliation":[{"name":"Department of Cellular Physiology and Metabolism, Faculty of Medicine University of Geneva  Geneva Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra","family":"Chassot","sequence":"additional","affiliation":[{"name":"Department of Cellular Physiology and Metabolism, Faculty of Medicine University of Geneva  Geneva Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Jellali","sequence":"additional","affiliation":[{"name":"Department of Cellular Physiology and Metabolism, Faculty of Medicine University of Geneva  Geneva Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicolas","family":"Liaudet","sequence":"additional","affiliation":[{"name":"Bioimaging Core Facility, Faculty of Medicine University of Geneva  Geneva Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Polat","sequence":"additional","affiliation":[{"name":"Department of Cellular Physiology and Metabolism, Faculty of Medicine University of Geneva  Geneva Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felix","family":"Baier","sequence":"additional","affiliation":[{"name":"Visceral Surgery and Medicine, Inselspital, Bern University Hospital, Department for BioMedical Research University of Bern  Bern Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deborah","family":"Stroka","sequence":"additional","affiliation":[{"name":"Visceral Surgery and Medicine, Inselspital, Bern University Hospital, Department for BioMedical Research University of Bern  Bern Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mikio","family":"Furuse","sequence":"additional","affiliation":[{"name":"Division of Cell Structure National Institute for Physiological Sciences  Okazaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric","family":"Feraille","sequence":"additional","affiliation":[{"name":"Department of Cellular Physiology and Metabolism, Faculty of Medicine University of Geneva  Geneva Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,4,3]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-006-0123-0"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-022-02732-5"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a002584"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00019.2012"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2014.08.011"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2014030284"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00204.2016"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00437.2010"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.2147\/IBPC.S13783"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00011.2014"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M107165200"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2003.tb07255.x"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.2040-4603.2023.tb00256.x"},{"issue":"3","key":"e_1_2_10_15_1","first-page":"F480","article-title":"Feedback Regulation of Na Channels in Rat CCT. 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