{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T13:21:47Z","timestamp":1787664107304,"version":"build-2736575974"},"reference-count":48,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["RS\u20102023\u201000219399"],"award-info":[{"award-number":["RS\u20102023\u201000219399"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["RS\u20102024\u201000454609"],"award-info":[{"award-number":["RS\u20102024\u201000454609"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012803","name":"Rosalind Franklin University of Medicine and Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012803","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Physiologica"],"published-print":{"date-parts":[[2026,9]]},"abstract":"ABSTRACT<\/jats:title>\n \n Hypoxia stimulates ventilation through a chemosensory reflex that is initiated by inhibition of the O\n 2<\/jats:sub>\n \u2010sensitive K\n +<\/jats:sup>\n channels such as K\n 2P<\/jats:sub>\n 3\/9 in carotid body Type 1 glomus (CB) cells. Because K\n 2P<\/jats:sub>\n 3\/9 activity is sensitive to cytosolic ATP concentration ([ATP]\n cyt<\/jats:sub>\n , K\n 1\/2<\/jats:sub>\n \u2009\u2248\u20092.5\u2009mM), it has been proposed that hypoxia suppresses mitochondrial ATP production and thereby reduces [ATP]\n cyt<\/jats:sub>\n to inhibit K\n 2P<\/jats:sub>\n 3\/9 activity. To test this hypothesis, we developed a K\n ATP<\/jats:sub>\n channel\u2010based bioassay using a patch\u2010cramming approach to measure [ATP]\n cyt<\/jats:sub>\n in living cells. Co\u2010expression of Kir6.2(\u0394C36\u2010R50G) and SUR1(G1485D) in HEK293 cells formed a functional K\n ATP<\/jats:sub>\n channel (designated R50G*) with markedly reduced ATP sensitivity. In inside\u2010out patches, normalized R50G* () activity showed ~100\u2010fold lower ATP sensitivity (K\n 1\/2<\/jats:sub>\n \u2009\u2248\u20092.0\u2009mM) than that of the wild\u2010type K\n ATP<\/jats:sub>\n (K\n 1\/2<\/jats:sub>\n \u2009\u2248\u200920\u2009\u03bcM). Inside\u2010out patches from HEK293 cells containing R50G* were crammed into CB cells under normoxia (21% O\n 2<\/jats:sub>\n ), exposing the patch to the CB cell cytoplasm. Based on activity in crammed CB cells, [ATP]\n cyt<\/jats:sub>\n was estimated to be ~4.9\u2009mM in normoxia. In CB cells, hypoxia (1% O\n 2<\/jats:sub>\n , 15\u2009s) did not affect activity, indicating that [ATP]\n cyt<\/jats:sub>\n remained stable during hypoxia. FCCP (1\u2009\u03bcM), a mitochondrial uncoupler, increased activity, which corresponded to [ATP]\n cyt<\/jats:sub>\n of ~3.7\u2009mM. As hypoxia (~1% O\n 2<\/jats:sub>\n ) inhibited K\n 2P<\/jats:sub>\n 3\/9 activity by 65% within 5\u2009s, our result demonstrates that inhibition of K\n 2P<\/jats:sub>\n 3\/9 by hypoxia is independent of [ATP]\n cyt<\/jats:sub>\n and that ATP depletion is unlikely to be the initiating signal for hypoxia\u2010induced excitation of CB cells.\n <\/jats:p>","DOI":"10.1111\/apha.70294","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:35:59Z","timestamp":1787031359000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Acute Hypoxia Inhibits\n \n K\n 2P<\/sub>\n 3\n <\/scp>\n \/9 (\n TASK1<\/scp>\n \/3) Independently of Cytosolic\n ATP<\/scp>\n in Carotid Body Type 1 Cells"],"prefix":"10.1111","volume":"242","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7402-7298","authenticated-orcid":false,"given":"Dawon","family":"Kang","sequence":"first","affiliation":[{"name":"Department 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