{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T09:01:27Z","timestamp":1790240487723,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004836","name":"Danmarks Frie Forskningsfond","doi-asserted-by":"publisher","award":["4002\u201000162B"],"award-info":[{"award-number":["4002\u201000162B"]}],"id":[{"id":"10.13039\/501100004836","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004836","name":"Danmarks Frie Forskningsfond","doi-asserted-by":"publisher","award":["8020\u201000254B"],"award-info":[{"award-number":["8020\u201000254B"]}],"id":[{"id":"10.13039\/501100004836","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Physiologica"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>Brown adipose tissue (BAT) specializes in energy consumption and thermogenesis in response to cold stress. Brown adipocytes (BA) express purinergic receptors, yet little is known about the source of extracellular ATP and the role of purinergic signaling, and the potential interaction with \u03b2\u2010adrenergic signaling. We aimed at elucidating whether: BA could release extracellular ATP; ATP\/purinergic signaling impacts \u03b2\u2010adrenergic responses; and the expression of purinergic receptors in BAT of mice and cultured BA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Real\u2010time ATP release was monitored in a mature BA cell model exposed to adrenergic agonists, insulin, glucose, and pharmacological inhibitors. Cellular assays included oxygen consumption, glycolysis, and lipolysis assays, and Ca\n                      <jats:sup>2+<\/jats:sup>\n                      imaging. The protein level of ATP\u2010release protein, pannexin\u20101, was determined. RNA\u2010sequencing was performed on mouse BAT and cultured BA.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      We show that \u03b2\u2010adrenergic stimulation of BA causes ATP release through pannexin\u20101 in a cAMP\u2010protein kinase A\u2010dependent manner. ATP release is increased with glucose but decreased with insulin, the latter due to phosphodiesterase 3 activation. \u03b2\u2010Adrenergic stimulation increases oxygen consumption, glycolysis, and lipolysis, while apyrase and pannexin\u20101 inhibitors decrease these effects. Both extracellular ATP and \u03b2\u2010adrenergic stimulation induce Ca\n                      <jats:sup>2+<\/jats:sup>\n                      signals, albeit with different kinetics. BAT from mice exposed to cold and BA treated with norepinephrine show regulation of several purinergic receptors.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The present study demonstrates that ATP is released from BA via pannexin\u20101 channels in response to \u03b2\u2010adrenergic stimulation, that extracellular ATP, acting through P2 receptor signaling, potentiates \u03b2\u2010adrenergic effects on BA function, and that expression of several purinergic receptors changes in response to \u03b2\u2010adrenergic stimulation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/apha.70307","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T07:46:59Z","timestamp":1788767219000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>ATP<\/scp>\n                    Release and Purinergic Signaling Potentiate \u03b2\u2010Adrenergic Effects on Brown Adipocytes"],"prefix":"10.1111","volume":"242","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2576-0183","authenticated-orcid":false,"given":"Marco","family":"Tozzi","sequence":"first","affiliation":[{"name":"Section for Cell Biology and Physiology, Department of Biology University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilie B.","family":"Berggreen","sequence":"additional","affiliation":[{"name":"Section for Cell Biology and Physiology, Department of Biology University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Section for Cell Biology and Physiology, Department of Biology University of Copenhagen  Copenhagen 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