{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T09:01:18Z","timestamp":1790240478026,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32601585"],"award-info":[{"award-number":["32601585"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Physiologica"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>Aberrant activation of dynamin\u2010related protein 1 (Drp1) promotes excessive mitochondrial reactive oxygen species (mtROS) production; however, whether the Drp1\u2010mtROS pathway mediates the renoprotective effects of aerobic exercise remains unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>High\u2010fat diet (HFD)\u2010fed mice were subjected to aerobic exercise or were intraperitoneally injected with the Drp1 inhibitor Mdivi\u20101 for 8\u2009weeks. Fat mass, serum biochemical parameters, renal histopathology, mtROS production, oxidative stress\u2010related markers, mitochondrial ultrastructure, mitochondrial membrane potential, ATP content, and protein expression were assessed. In parallel, Drp1 was knocked down in palmitic acid (PA)\u2010treated HK\u20102 cells to further evaluate the role of the Drp1\u2010mtROS pathway in lipid overload\u2010induced renal tubular cell injury.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Aerobic exercise reduced body weight and fat mass, improved circulating lipid profiles, and decreased serum creatinine and blood urea nitrogen levels in HFD\u2010fed mice. Exercise also alleviated renal tubular injury, mesangial expansion, interstitial fibrosis, podocyte foot process effacement, and renal lipid accumulation. Mechanistically, aerobic exercise suppressed Drp1 phosphorylation at Ser616, reduced renal mtROS production and oxidative stress, improved mitochondrial morphology, restored mitochondrial membrane potential and ATP production, and modulated the expression of lipid\u2010metabolism\u2010related proteins. Pharmacological inhibition of Drp1 with Mdivi\u20101 largely reproduced the metabolic, mitochondrial, and renoprotective effects of aerobic exercise. Furthermore, Drp1 knockdown attenuated PA\u2010induced mtROS accumulation, oxidative stress, mitochondrial dysfunction, and lipid deposition in HK\u20102 cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Aerobic exercise exerts a protective effect on the kidneys of HFD\u2010fed mice, at least in part, by inhibiting the Drp1\u2010mtROS pathway. This provides insights into the benefits of aerobic exercise in obesity\u2010related nephropathy.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/apha.70310","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T08:21:46Z","timestamp":1788769306000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Aerobic Exercise Ameliorates Renal Injury in High\u2010Fat Diet\u2010Fed Mice: A Novel Effect Mediated by the Drp1\u2010mtROS Pathway"],"prefix":"10.1111","volume":"242","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-6111-9531","authenticated-orcid":false,"given":"Huiqian","family":"Ren","sequence":"first","affiliation":[{"name":"School of Physical Education and Health Engineering Taiyuan University of Technology  Taiyuan Shanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haofeng","family":"Sun","sequence":"additional","affiliation":[{"name":"School 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