{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T01:12:36Z","timestamp":1784682756373,"version":"3.55.0"},"reference-count":14,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,9,25]],"date-time":"2012-09-25T00:00:00Z","timestamp":1348531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:p>The subcultivation of positive blood cultures for organism identification is a time\u2010consuming process. We explored the use of fluorescent quantum dots (<jats:styled-content style=\"fixed-case\">QDs<\/jats:styled-content>) as probes for the improved detection and identification of <jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>taphylococcus aureus<\/jats:italic> directly from positive blood culture media. Immunofluorescence probes were synthesized by linking biotin\u2010conjugated <jats:styled-content style=\"fixed-case\">QDs<\/jats:styled-content> to streptavidin\u2010conjugated <jats:styled-content style=\"fixed-case\">IgG<\/jats:styled-content> molecules. This <jats:styled-content style=\"fixed-case\">QDs<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">IgG<\/jats:styled-content> complex is capable of binding anti\u2010<jats:styled-content style=\"fixed-case\">SPA<\/jats:styled-content> antibodies which specifically bind to a cell surface protein of <jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>. aureus<\/jats:italic>. The specificity of the method was confirmed using reference strains. Among 372 positive blood cultures, 73 containing gram\u2010positive cocci in clusters were investigated. Relative to the standard culture method, the immunofluorescence assay showed 90.5% (19\/21) sensitivity and 100% (52\/52) specificity for <jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>. aureus<\/jats:italic>. These results suggest that this immunofluorescence assay allows the rapid identification of <jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>. aureus<\/jats:italic> directly from specimens.<\/jats:p>","DOI":"10.1111\/apm.12005","type":"journal-article","created":{"date-parts":[[2012,9,25]],"date-time":"2012-09-25T17:49:20Z","timestamp":1348595360000},"page":"348-352","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Rapid identification of <i><scp>S<\/scp>taphylococcus aureus<\/i> directly from positive blood culture media using quantum dots as fluorescence probes"],"prefix":"10.1111","volume":"121","author":[{"given":"Qing","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Renmin Hospital of Wuhan University Wuhan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Renmin Hospital of Wuhan University Wuhan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Renmin Hospital of Wuhan University Wuhan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ze G.","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Renmin Hospital of Wuhan University Wuhan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bi H.","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Chemistry and Molecular Sciences Wuhan University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,9,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1086\/598189"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.md.0000091184.93122.09"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.27.6.1342-1345.1989"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1163\/092050610X528570"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2008.10.025"},{"key":"e_1_2_6_7_1","first-page":"143","article-title":"Immunochemical studies on the specific agglutinogens of Staphylococcus aureus. 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